Protein kinase Cα/Β inhibitor Gö6976 promotes PC12 cell adhesion and spreading through membrane recruitment and activation of protein kinase Cδ

Experimental Cell Research

Volumn 319, Issue 3, 2013, Pages 153-160

  Youn Jung, Sung ^a   Bok Kim, O ^a   Kang, Hyun Ki ^a   Jang, Da Hyun ^a   Min, Byung Moo ^a   Yu, Frank H ^b  

^a BK 21 Plus   (South Korea)

^b Seoul National University   (South Korea)

Author keywords

Cellular activity; Laminin; PKC inhibitor G 6976; PKC activation; Small GTPases

Indexed keywords

12 (2 CYANOETHYL) 6,7,12,13 TETRAHYDRO 13 METHYL 5 OXOINDOLO[2,3 A]PYRROLO[3,4 C]CARBAZOLE; FOCAL ADHESION KINASE; GUANOSINE TRIPHOSPHATASE; LAMININ; PROTEIN CDC42; PROTEIN KINASE C DELTA; RAC1 PROTEIN; ROTTLERIN; SMALL INTERFERING RNA;

ANIMAL CELL; ARTICLE; AUTOPHOSPHORYLATION; CELL ADHESION; CELL FUNCTION; CELL MEMBRANE; CELL SPREADING; CONCENTRATION RESPONSE; CONTROLLED STUDY; ENZYME ACTIVATION; ENZYME PHOSPHORYLATION; FIBROBLAST; GENETIC TRANSFECTION; LAMELLIPODIUM; NONHUMAN; PC12 CELL; PRIORITY JOURNAL; PROTEIN EXPRESSION; RAT; SIGNAL TRANSDUCTION; STRESS FIBER;

EID: 84872371837     PISSN: 00144827     EISSN: 10902422     Source Type: Journal    
DOI: 10.1016/j.yexcr.2012.10.003     Document Type: Article

References (36)

1. Leivo I., Engvall E. Merosin, a protein specific for basement membranes of Schwann cells, striated muscle, and trophoblast, is expressed late in nerve and muscle development. Proc. Natl. Acad. Sci. USA 1988, 85:1544-1548.
2. Suzuki N., Yokoyama F., Nomizu M. Functional sites in the laminin alpha chains. Connect. Tissue Res. 2005, 46:142-152.
3. Schwartz M.A., Schaller M.D., Ginsberg M.H. Integrins: emerging paradigms of signal transduction. Annu. Rev. Cell Dev. Biol. 1995, 11:549-599.
4. Aplin A.E., Howe A., Alahari S.K., Juliano R.L. Signal transduction and signal modulation by cell adhesion receptors: the role of integrins, cadherins, immunoglobulin-cell adhesion molecules, and selectins. Pharmacol. Rev. 1998, 50:197-263.
5. Schlaepfer D.D., Hunter T. Integrin signalling and tyrosine phosphorylation: just the FAKs?. Trends Cell Biol. 1998, 8:151-157.
6. Jung S.Y., Kim J.M., Min S.K., Kim O.B., Jang da H., Kang H.K., Min B.M. The potential of laminin-2-biomimetic short peptide to promote cell adhesion, spreading and migration by inducing membrane recruitment and phosphorylation of PKCδ. Biomaterials 2012, 33:3967-3979.
7. Min S.K., Lee S.C., Hong S.D., Chung C.P., Park W.H., Min B.M. The effect of a laminin-5-derived peptide coated onto chitin microfibers on re-epithelialization in early-stage wound healing. Biomaterials 2010, 31:4725-4730.
8. Kadoya Y., Mochizuki M., Nomizu M., Sorokin L., Yamashina S. Role for laminin-alpha5 chain LG4 module in epithelial branching morphogenesis. Dev. Biol. 2003, 263:153-164.
9. Hibino S., Shibuya M., Engbring J.A., Mochizuki M., Nomizu M., Kleinman H.K. Identification of an active site on the laminin alpha5 chain globular domain that binds to CD44 and inhibits malignancy. Cancer Res. 2004, 64:4810-4816.
10. Hibino S., Shibuya M., Hoffman M.P., Engbring J.A., Hossain R., Mochizuki M., Kudoh S., Nomizu M., Kleinman H.K. Laminin alpha5 chain metastasis- and angiogenesis-inhibiting peptide blocks fibroblast growth factor 2 activity by binding to the heparan sulfate chains of CD44. Cancer Res. 2005, 65:10494-10501.
11. Ron D., Kazanietz M.G. New insights into the regulation of protein kinase C and novel phorbol ester receptors. FASEB J. 1999, 13:1658-1676.
12. Huang X., Wu J., Spong S., Sheppard D. The integrin alphavbeta6 is critical for keratinocyte migration on both its known ligand, fibronectin, and on vitronectin. J. Cell Sci. 1998, 111:2189-2195.
13. Woods A., Couchman J.R. Protein kinase C involvement in focal adhesion formation. J. Cell Sci. 1992, 101:277-290.
14. Disatnik M.H., Boutet S.C., Lee C.H., Mochly-Rosen D., Rando T.A. Sequential activation of individual PKC isozymes in integrin-mediated muscle cell spreading: a role for MARCKS in an integrin signaling pathway. J. Cell Sci. 2002, 115:2151-2163.
15. Kim J.M., Park W.H., Min B.M. The PPFLMLLKGSTR motif in globular domain 3 of the human laminin-5 alpha3 chain is crucial for integrin alpha3beta1 binding and cell adhesion. Exp. Cell Res. 2005, 304:317-327.
16. Okazaki I., Suzuki N., Nishi N., Utani A., Matsuura H., Shinkai H., Yamashita H., Kitagawa Y., Nomizu M. Identification of biologically active sequences in the laminin alpha 4 chain G domain. J. Biol. Chem. 2002, 277:37070-37078.
17. Timar J., Trikha M., Szekeres K., Bazaz R., Tovari J., Silletti S., Raz A., Honn K.V. Autocrine motility factor signals integrin-mediated metastatic melanoma cell adhesion and invasion. Cancer Res. 1996, 56:1902-1908.
18. Mandil R., Ashkenazi E., Blass M., Kronfeld I., Kazimirsky G., Rosenthal G., Umansky F., Lorenzo P.S., Blumberg P.M., Brodie C. Protein kinase Cα and protein kinase Cδ play opposite roles in the proliferation and apoptosis of glioma cells. Cancer Res. 2001, 61:4612-4619.
19. Dovas A., Yoneda A., Couchman J.R. PKCbeta-dependent activation of RhoA by syndecan-4 during focal adhesion formation. J. Cell Sci. 2006, 119:2837-2846.
20. Martiny-Baron G., Kazanietz M.G., Mischak H., Blumberg P.M., Kochs G., Hug H., Marme D., Schachtele C. Selective inhibition of protein kinase C isozymes by the indolocarbazole Gö6976. J. Biol. Chem. 1993, 268:9194-9197.
21. Ng T., Shima D., Squire A., Bastiaens P.I., Gschmeissner S., Humphries M.J., Parker P.J. PKCα regulates beta1 integrin-dependent cell motility through association and control of integrin traffic. EMBO J. 1999, 18:3909-3923.
22. Lewis J.M., Cheresh D.A., Schwartz M.A. Protein kinase C regulates alpha v beta 5-dependent cytoskeletal associations and focal adhesion kinase phosphorylation. J. Cell Biol. 1996, 134:1323-1332.
23. Hauzenberger D., Klominek J., Holgersson J., Bergstrom S.E., Sundqvist K.G. Triggering of motile behavior in T lymphocytes via cross-linking of alpha 4 beta 1 and alpha L beta 2. J. Immunol. 1997, 158:76-84.
24. Kornberg L., Earp H.S., Parsons J.T., Schaller M., Juliano R.L. Cell adhesion or integrin clustering increases phosphorylation of a focal adhesion-associated tyrosine kinase. J. Biol. Chem. 1992, 267:23439-23442.
25. Hanks S.K., Calalb M.B., Harper M.C., Patel S.K. Focal adhesion protein-tyrosine kinase phosphorylated in response to cell attachment to fibronectin. Proc. Natl. Acad. Sci. USA 1992, 89:8487-8491.
26. Ridley A.J., Schwartz M.A., Burridge K., Firtel R.A., Ginsberg M.H., Borisy G., Parsons J.T., Horwitz A.R. Cell migration: integrating signals from front to back. Science 2003, 302:1704-1709.
27. Barry S.T., Critchley D.R. The RhoA-dependent assembly of focal adhesions in Swiss 3T3 cells is associated with increased tyrosine phosphorylation and the recruitment of both pp125FAK and protein kinase C-delta to focal adhesions. J. Cell Sci. 1994, 107:2033-2045.
28. Matsuo N., Terao M., Nabeshima Y., Hoshino M. Roles of STEF/Tiam1, guanine nucleotide exchange factors for Rac1, in regulation of growth cone morphology. Mol. Cell Neurosci. 2003, 24:69-81.
29. Regent M., Planus E., Bouin A.P., Bouvard D., Brunner M., Faurobert E., Millon-Fremillon A., Block M.R., Albiges-Rizo C. Specificities of beta1 integrin signaling in the control of cell adhesion and adhesive strength. Eur. J. Cell Biol. 2011, 90:261-269.
30. Mochly-Rosen D. Localization of protein kinases by anchoring proteins: a theme in signal transduction. Science 1995, 268:247-251.
31. Besson A., Wilson T.L., Yong V.W. The anchoring protein RACK1 links protein kinase Cepsilon to integrin beta chains. Requirements for adhesion and motility. J. Biol. Chem. 2002, 277:22073-22084.
32. Haller H., Lindschau C., Maasch C., Olthoff H., Kurscheid D., Luft F.C. Integrin-induced protein kinase Cα and Ce{open} translocation to focal adhesions mediates vascular smooth muscle cell spreading. Circ. Res. 1998, 82:157-165.
33. Behrens M.M., Strasser U., Choi D.W. Gö6976 is a potent inhibitor of neurotrophin-receptor intrinsic tyrosine kinase. J. Neurochem. 1999, 72:919-924.
34. Grandage V.L., Everington T., Linch D.C., Khwaja A. Gö6976 is a potent inhibitor of the JAK 2 and FLT3 tyrosine kinases with significant activity in primary acute myeloid leukaemia cells. Br. J. Haematol. 2006, 135:303-316.
35. Robinson J.W., Lou X., Potter L.R. The indolocarbazole, Gö6976, inhibits guanylyl cyclase-A and -B. Br. J. Pharmacol. 2011, 164:499-506.
36. Chang F., Lemmon C.A., Park D., Romer L.H. FAK potentiates Rac1 activation and localization to matrix adhesion sites: a role for ΒaPIX. Mol. Biol. Cell 2007, 18:253-264.