Silica nanoparticles-induced cytotoxicity, oxidative stress and apoptosis in cultured A431 and A549 cells

Human and Experimental Toxicology

Volumn 32, Issue 2, 2013, Pages 186-195

  Ahamed, Maqusood ^a  

^a KING SAUD UNIVERSITY   (Saudi Arabia)

Author keywords

A431 cells; A549 cells; apoptosis; ROS; SiO2 NPs

Indexed keywords

3 (4,5 DIMETHYL 2 THIAZOLYL) 2,5 DIPHENYLTETRAZOLIUM BROMIDE; CASPASE 3; CASPASE 9; GLUTATHIONE; LACTATE DEHYDROGENASE; LIPID; MESSENGER RNA; NANOPARTICLE; REACTIVE OXYGEN METABOLITE; SILICA NANOPARTICLE; SILICON DIOXIDE; UNCLASSIFIED DRUG;

APOPTOSIS; ARTICLE; CELL VIABILITY; CONTROLLED STUDY; CYTOTOXICITY; ENZYME ACTIVITY; EPITHELIUM CELL; HUMAN; HUMAN CELL; LIPID PEROXIDATION; LUNG ALVEOLUS EPITHELIUM; OXIDATIVE STRESS; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; SKIN EPITHELIUM; UPREGULATION;

APOPTOSIS; CASPASE 3; CASPASE 9; CELL LINE, TUMOR; CELL SURVIVAL; GLUTATHIONE; HUMANS; LIPID PEROXIDATION; NANOPARTICLES; OXIDATIVE STRESS; REACTIVE OXYGEN SPECIES; RNA, MESSENGER; SILICON DIOXIDE;

EID: 84872360422     PISSN: 09603271     EISSN: 14770903     Source Type: Journal    
DOI: 10.1177/0960327112459206     Document Type: Article

References (41)

1. Zhang FF, Wan Q, Li CX, Wang XL, Zhu ZQ, Xian YZ, et al. Simultaneous assay of glucose, lactate, L-glutamate and hypoxanthine levels in a rat striatum using enzyme electrodes based on neutral red-doped silica nanoparticles. Anal Bioanal Chem. 2004 ; 380: 637-642
2. Santra S, Zhang P, Wang K, Tapec R, Tan W. Conjugation of biomolecules with luminophore-doped silica nanoparticles for photostable biomarkers. Anal Biochem. 2001 ; 73: 4988-4993
3. Hirsch LR, Stafford RJ, Bankson JA, Sershen SR, Rivera B, Price RE, et al. Nanoshell-mediated near infrared thermal therapy of tumors under magnetic resonance guidance. Proc Natl Acad Sci USA. 2003 ; 100: 13549-13554
4. Bharali DJ, Klejbor I, Stachowiak EK, Dutta P, Roy I, Kaur N, et al. Organically modified silica nanoparticles: a nonviral vector for in vivo gene delivery and expression in the brain. Proc Natl Acad Sci USA. 2005 ; 102: 11539-11544
5. Slowing II, Vivero-Escoto JL, Wu CW, Lin VSY. Mesoporous silica nanoparticles as controlled release drug delivery and gene transfection carriers. Adv Drug Deliv Rev. 2008 ; 60: 1278-1288
6. Barik TK, Sahu B, Swain V. Nanosilica-from medicine to pest control. Parasitol Res. 2008 ; 103: 253-258
7. Oberdorster G, Oberdorster E, Oberdorster J. Nanotoxicology: an emerging discipline evolving from studies of ultrafine particles. Environ Health Perspect. 2005 ; 113: 823-839
8. Kaewamatawong T, Shimada A, Okajima M, Inoue H, Morita T, Inoue K, et al. Acute and subacute pulmonary toxicity of low dose of ultrafine colloidal silica particles in mice after intratracheal instillation. Toxicol Pathol. 2006 ; 34: 958-965
9. Park EJ, Park K. Oxidative stress, pro-inflammatory responses induced by silica nanoparticles in vivo and in vitro. Toxicol Lett. 2009 ; 184: 18-25
10. Liu T, Li L, Teng X, Huang X, Liu H, Chen D, et al. Single and repeated dose toxicity of mesoporous hollow silica nanoparticles in intravenously exposed mice. Biomaterials. 2011 ; 32: 1657-1668
11. Lison D, Thomassen LCJ, Rabolli V, Gonzalez L, Napierska D, Seo JW, et al. Nominal and effective dosimetry of silica nanoparticles in cytotoxicity assays. Toxicol Sci. 2008 ; 104: 155-162
12. Napierska D, Thomassen LC, Rabolli V, Lison D, Gonzalez L, Kirsch-Volders M, et al. Size-dependent cytotoxicity of monodisperse silica nanoparticles in human endothelial cells. Small. 2009 ; 5: 846-853
13. Wang F, Gao F, Lan M, Yuan H, Huang Y, Liu J. Oxidative stress contributes to silica nanoparticle-induced cytotoxicity in human embryonic kidney cells. Toxicol In Vitro. 2009 ; 23: 808-815
14. Wang F, Jiao C, Liu J, Yuan H, Lan M, Gao F. Oxidative mechanisms contribute to nanosize silican dioxide-induced developmental neurotoxicity in PC12 cells. Toxicol In Vitro. 2011 ; 25: 1548-1556
15. Ahmad J, Ahamed M, Akhtar MJ, Alrokayan SA, Siddiqui MA, Musarrat J, et al. Apoptosis induction by amorphous silica nanoparticles mediated through reactive oxygen species generation in human liver cell line HepG2. Toxicol Appl Pharmacol. 2012 ; 259: 160-168
16. Arora S, Jain J, Rajwade JM, Paknikar KM. Cellular responses induced by silver nanoparticles: in vitro studies. Toxicol Lett. 2008 ; 179: 93-100
17. Ahamed M, Siddiqui MA, Akhtar MJ, Ahmad I, Pant AB, Alhadlaq HA. Genotoxic potential of copper oxide nanoparticles in human lung epithelial cells. Biochem Biophys Res Commun. 2010 ; 396: 578-583
18. Ahamed M, Akhtar MJ, Siddiqui MA, Ahmad J, Musarrat J, Al-Khedhairy AA, et al. Oxidative stress mediated apoptosis induced by nickel ferrite nanoparticles in cultured A549 cells. Toxicology. 2011 ; 283: 101-108
19. Ahamed M. Toxic response of nickel nanoparticles in human lung epithelial A549 cells. Toxicol in Vitro. 2011 ; 25: 930-936
20. Ahamed M, Akhtar MJ, Raja M, Ahmad I, Siddiqui MKJ, AlSalhi MS, et al. ZnO nanorod-induced apoptosis in human alveolar adenocarcinoma cells via p53, survivin and bax/bcl-2 pathways: role of oxidative stress. Nanomedicine: NBM. 2011 ; 7: 904-913
21. Wroblewski F, LaDue JS. Lactate dehydrogenase activity in blood. Proc Soc Exp Biol Med. 1955 ; 90: 210-213
22. Wang H, Joseph JA. Quantifying cellular oxidative stress by dichlorofluorescin assay using microplate reader. Free Radic Biol Med. 1999 ; 27: 612-616
23. Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem. 1979 ; 95: 351-358
24. Ellman GI. Tissue sulfhydryl groups. Arch Biochem Biophys. 1959 ; 82: 70-77
25. Berasain C, Garcia-Trevijano ER, Castillo J, Erroba E, Santamaria M, Lee DC. Novel role for amphiregulin in protection from liver injury. J Biol Chem. 2005 ; 280: 19012-19020
26. 2, and ZnO water suspensions. Water Res. 2006 ; 40: 3527-3532
27. Fent K, Weisbrod CJ, Wirth-Heller A, Pieles U. Assessment of uptake and toxicity of fluorescent silica nanoparticles in zebrafish (Danio rerio) early life stages. Aquat Toxicol. 2010 ; 100: 218-228
28. Hoecke KV, De Schamphelaere KAC, der Meeren PV, Lucas S, Janssen CR. Ecotoxicity of silica nanoparticles to the green alga Pseudokirchneriella subcapitata: importance of surface area. Environ Toxicol Chem. 2008 ; 27: 1948-1957
29. Nishimori H, Kondoh M, Isoda K, Tsunoda S, Tsutsumi Y, Yagi K. Silica nanoparticles as hepatotoxicants. Eur J Pharm Biopharm. 2009 ; 72: 496-501
30. Balduzzi M, Diociaiuti M, Berardis BD. In vitro effects on macrophages induced by noncytotoxic doses of silica particles possibly relevant to ambient exposure. Environ Res. 2004 ; 96: 62-71
31. Hussain SM, Hess KL, Gearhart JM.. In vitro toxicity of nanoparticles in BRL 3A rat liver cells. Toxicol In Vitro. 2005 ; 19: 975-983
32. Akhtar MJ, Ahamed M, Kumar S, Siddiqui H, Patil G, Ashquin M, et al. Nanotoxicity of pure silica mediated through oxidant generation rather than glutathione depletion in human lung epithelial cells. Toxicology. 2010 ; 276: 95-102
33. Akhtar MJ, Kumar S, Murthy RC, Ashquin M, Khan MI, Patil G, et al. The primary role of iron-mediated lipid peroxidation in the differential cytotoxicity caused by two varieties of talc nanoparticles on A549 cells and lipid peroxidation inhibitory effect exerted by ascorbic acid. Toxicol In Vitro. 2010 ; 24: 1139-1147
34. Nel A, Xia T, Madler L, Lin N. Toxic potential of materials at the nano level. Science. 2006 ; 311: 622-627
35. Akhtar MJ, Ahamed M, Kumar S, Ahmad J, Khan MAM, Alrokayan SA. Zinc oxide nanoparticles selectively induces apoptosis in cancer cells through reactive oxygen species. Int J Nanomed. 2012 ; 7: 845-857
36. Ahamed M, AlSalhi MS, Siddiqui MKJ. Silver nanoparticle applications and human health. Clin Chim Acta. 2011 ; 411: 1841-1848
37. Lunov O, Syrovets T, Buchele B, Jiang X, Rocker C, Tron K, et al. The effect of carboxydextran-coated superparamagnetic iron oxide nanoparticles on c-Jun N-terminal kinase-mediated apoptosis in human macrophages. Biomaterials. 2010 ; 31: 5063-5071
38. Stone V, Donaldson K. Nanotoxicology: signs of stress. Nat Nanotech. 2006 ; 1: 23-24
39. Fuentes-Prior P, Salvesen GS. The protein structures that shape caspase activity, specificity, activation and inhibition. J Biochem. 2004 ; 384: 201-232
40. Youle RJ, Strasser A. The BCL-2 protein family: opposing activities that mediate cell death. Nat Rev Mol Cell Biol. 2008 ; 9: 47-59
41. Timmer JC, Salvesen GS. Caspase substrates. Cell Death Differ. 2007 ; 14: 66-72