Mechanistic Target of Rapamycin (Mtor) Is Essential for Murine Embryonic Heart Development and Growth

PLoS ONE

Volumn 8, Issue 1, 2013, Pages

  Zhu, Yi ^a   Pires, Karla M P ^a,b   Whitehead, Kevin J ^a   Olsen, Curtis D ^a   Wayment, Benjamin ^a   Zhang, Yi Cheng ^a   Bugger, Heiko ^a,d   Ilkun, Olesya ^a   Litwin, Sheldon E ^a,e   Thomas, George ^c   Kozma, Sara C ^c   Abel, E Dale ^a  

^a UNIVERSITY OF UTAH SCHOOL OF MEDICINE   (United States)

^b STATE UNIVERSITY OF RIO DE JANEIRO   (Brazil)

^c UNIVERSITY OF CINCINNATI COLLEGE OF MEDICINE   (United States)

^d UNIVERSITY OF FREIBURG   (Germany)

^e MEDICAL COLLEGE OF GEORGIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CRE RECOMBINASE; MAMMALIAN TARGET OF RAPAMYCIN; MYOSIN HEAVY CHAIN ALPHA;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL TISSUE; APOPTOSIS; ARTICLE; CELL EXPANSION; CELL LOSS; CELL PROLIFERATION; CELL SURVIVAL; CONTROLLED STUDY; EMBRYO; EMBRYO DEATH; EMBRYO DEVELOPMENT; ENZYME ACTIVITY; GENE DELETION; GESTATION PERIOD; HEART DEVELOPMENT; HEART MUSCLE CELL; INNER CELL MASS; MOUSE; NONHUMAN; PROTEIN DEFICIENCY; PROTEIN EXPRESSION; TISSUE BLOOD FLOW;

ANIMALS; BLOTTING, WESTERN; EMBRYONIC DEVELOPMENT; FEMALE; HEART; MICE; MICE, KNOCKOUT; TOR SERINE-THREONINE KINASES;

MURINAE;

EID: 84872354779     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0054221     Document Type: Article

References (28)

1. Laplante M, Sabatini DM, (2009) mTOR signaling at a glance. J Cell Sci 122: 3589-3594.
2. Murakami M, Ichisaka T, Maeda M, Oshiro N, Hara K, et al. (2004) mTOR is essential for growth and proliferation in early mouse embryos and embryonic stem cells. Mol Cell Biol 24: 6710-6718.
3. Gangloff YG, Mueller M, Dann SG, Svoboda P, Sticker M, et al. (2004) Disruption of the mouse mTOR gene leads to early postimplantation lethality and prohibits embryonic stem cell development. Mol Cell Biol 24: 9508-9516.
4. Dunlop EA, Tee AR, (2009) Mammalian target of rapamycin complex 1: signalling inputs, substrates and feedback mechanisms. Cell Signal 21: 827-835.
5. Bentzinger CF, Romanino K, Cloëtta D, Lin S, Mascarenhas JB, et al. (2008) Skeletal Muscle-Specific Ablation of raptor, but Not of rictor, Causes Metabolic Changes and Results in Muscle Dystrophy. Cell Metabolism 8: 411-424.
6. Polak P, Cybulski N, Feige JN, Auwerx J, Ruegg MA, et al. (2008) Adipose-specific knockout of raptor results in lean mice with enhanced mitochondrial respiration. Cell Metab 8: 399-410.
7. Sarbassov DD, Guertin DA, Ali SM, Sabatini DM, (2005) Phosphorylation and regulation of Akt/PKB by the rictor-mTOR complex. Science 307: 1098-1101.
8. Jacinto E, Facchinetti V, Liu D, Soto N, Wei S, et al. (2006) SIN1/MIP1 maintains rictor-mTOR complex integrity and regulates Akt phosphorylation and substrate specificity. Cell 127: 125-137.
9. Lazorchak AS, Liu D, Facchinetti V, Di Lorenzo A, Sessa WC, et al. (2010) Sin1-mTORC2 suppresses rag and il7r gene expression through Akt2 in B cells. Mol Cell 39: 433-443.
10. Cybulski N, Hall MN, (2009) TOR complex 2: a signaling pathway of its own. Trends Biochem Sci 34: 620-627.
11. Cybulski N, Polak P, Auwerx J, Ruegg MA, Hall MN, (2009) mTOR complex 2 in adipose tissue negatively controls whole-body growth. Proc Natl Acad Sci U S A 106: 9902-9907.
12. Risson V, Mazelin L, Roceri M, Sanchez H, Moncollin V, et al. (2009) Muscle inactivation of mTOR causes metabolic and dystrophin defects leading to severe myopathy. J Cell Biol 187: 859-874.
13. Shiojima I, Sato K, Izumiya Y, Schiekofer S, Ito M, et al. (2005) Disruption of coordinated cardiac hypertrophy and angiogenesis contributes to the transition to heart failure. J Clin Invest 115: 2108-2118.
14. Shen WH, Chen Z, Shi S, Chen H, Zhu W, et al. (2008) Cardiac restricted overexpression of kinase-dead mammalian target of rapamycin (mTOR) mutant impairs the mTOR-mediated signaling and cardiac function. J Biol Chem 283: 13842-13849.
15. Zhang D, Contu R, Latronico MV, Zhang JL, Rizzi R, et al. (2010) MTORC1 regulates cardiac function and myocyte survival through 4E-BP1 inhibition in mice. J Clin Invest 120: 2805-2816.
16. Hentges KE, Sirry B, Gingeras AC, Sarbassov D, Sonenberg N, et al. (2001) FRAP/mTOR is required for proliferation and patterning during embryonic development in the mouse. Proc Natl Acad Sci U S A 98: 13796-13801.
17. Kilkenny C, Browne W, Cuthill IC, Emerson M, Altman DG, (2011) Animal research: reporting in vivo experiments-the ARRIVE guidelines. J Cereb Blood Flow Metab 31: 991-993.
18. Yin Z, Jones GN, Towns WH, 2nd, Zhang X, Abel ED, et al (2008) Heart-specific ablation of Prkar1a causes failure of heart development and myxomagenesis. Circulation 117: 1414-1422.
19. Valencik ML, McDonald JA, (2001) Codon optimization markedly improves doxycycline regulated gene expression in the mouse heart. Transgenic Res 10: 269-275.
20. Wang Q, Chan TR, Hilgraf R, Fokin VV, Sharpless KB, et al. (2003) Bioconjugation by copper(I)-catalyzed azide-alkyne [3+2] cycloaddition. J Am Chem Soc 125: 3192-3193.
21. Salic A, Mitchison TJ, (2008) A chemical method for fast and sensitive detection of DNA synthesis in vivo. Proc Natl Acad Sci U S A 105: 2415-2420.
22. Altunkaynak ME, Altunkaynak BZ, Unal D, Yildirim S, Can I, et al. (2011) Stereological and histological analysis of the developing rat heart. Anat Histol Embryol 40: 402-410.
23. Whitehead KJ, Chan AC, Navankasattusas S, Koh W, London NR, et al. (2009) The cerebral cavernous malformation signaling pathway promotes vascular integrity via Rho GTPases. Nat Med 15: 177-184.
24. Gaussin V, Van de Putte T, Mishina Y, Hanks MC, Zwijsen A, et al. (2002) Endocardial cushion and myocardial defects after cardiac myocyte-specific conditional deletion of the bone morphogenetic protein receptor ALK3. Proc Natl Acad Sci U S A 99: 2878-2883.
25. Cunningham JT, Rodgers JT, Arlow DH, Vazquez F, Mootha VK, et al. (2007) mTOR controls mitochondrial oxidative function through a YY1-PGC-1alpha transcriptional complex. Nature 450: 736-740.
26. Ieda M, Tsuchihashi T, Ivey KN, Ross RS, Hong TT, et al. (2009) Cardiac fibroblasts regulate myocardial proliferation through beta1 integrin signaling. Dev Cell 16: 233-244.
27. Lyons GE, Schiaffino S, Sassoon D, Barton P, Buckingham M, (1990) Developmental regulation of myosin gene expression in mouse cardiac muscle. J Cell Biol 111: 2427-2436.
28. Belke DD, Betuing S, Tuttle MJ, Graveleau C, Young ME, et al. (2002) Insulin signaling coordinately regulates cardiac size, metabolism, and contractile protein isoform expression. J Clin Invest 109: 629-639.