Codon optimization markedly improves doxycycline regulated gene expression in the mouse heart

Transgenic Research

Volumn 10, Issue 3, 2001, Pages 269-275

  Valencik, M L ^a   McDonald, J A ^a  

^a MAYO GRADUATE SCHOOL   (United States)

Author keywords

Conditional transgene expression; Doxycycline; Heart; Tetracycline

Indexed keywords

CODON; DOXYCYCLINE; GENE EXPRESSION REGULATION; GENE INDUCTION; GENETIC LINE; HEMIZYGOSITY; LETHAL MUTANT; MYOSIN HEAVY CHAIN; REVERSE TRANSCRIPTION; TETRACYCLINE; TRANSGENE; TRANSGENIC ANIMAL; TRANSGENICS;

ANIMALS; ANTIBODIES, MONOCLONAL; BASE SEQUENCE; BLOTTING, NORTHERN; CELLS, CULTURED; CODON; DOXYCYCLINE; GENE EXPRESSION REGULATION; GENES, REPORTER; IMMUNOBLOTTING; LUCIFERASES; MICE; MOLECULAR SEQUENCE DATA; MYOCARDIUM; MYOSIN HEAVY CHAINS; OPEN READING FRAMES; PROMOTER REGIONS (GENETICS); RNA, MESSENGER; TRANS-ACTIVATORS; TRANSGENES;

ANIMALIA; RODENTIA;

EID: 0034969734     PISSN: 09628819     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1016601928465     Document Type: Article

References (14)

1. Co-regulation of two gene activities by tetracycline via a bidirectional promoter
2. Expression of protein kinase Cβ in the heart causes hypertrophy in adult mice and sudden death in neonates
3. Temporal control of gene expression in transgenic mice by a tetracycline-responsive promoter
4. Production of transgenic mice
5. Tight control of gene expression in mammalian cells by tetracycline-responsive promoters
6. Transcriptional activation by tetracyclines in mammalian cells
7. Isolation and characterization of the mouse cardiac myosin heavy chain genes
8. Single subunit chimeric integrins as mimics and inhibitors of endogenous integrin functions in receptor localization, cell spreading and migration, and matrix assembly
9. Isolation of high molecular weight DNA for reliable genotyping of transgenic mice
10. 'Cre'-ating mouse mutants-a meeting review on conditional mouse genetics
11. Isolation and characterization of chinese hamster ovary cell variants deficient in the expression of fibronectin receptor
12. The Yersinia pseudotuberculosis invasin protein and human fibronectin bind to mutually exclusive sites on the α5β1 integrin receptor
13. Codon optimization, genetic insulation, and an rtTA reporter improve performance of the tetracycline switch
14. Evidence for a factor required for transcriptional stimulation by the chimeric acidic activator GAL-VP16 in HeLa cell extracts