Cloning, overexpression, purification, and characterization of a polyextremophilic β-galactosidase from the Antarctic haloarchaeon Halorubrum lacusprofundi

BMC Biotechnology

Volumn 13, Issue , 2013, Pages

  Karan, Ram ^a   Capes, Melinda D ^a   DasSarma, Priya ^a   DasSarma, Shiladitya ^a  

^a UNIVERSITY OF MARYLAND SCHOOL OF MEDICINE   (United States)

Author keywords

Biofuels; Extremozymes; Halophiles; Polyextremophiles; Protein stability; Psychrophiles

Indexed keywords

EXTREMOZYMES; HALOPHILES; POLYEXTREMOPHILES; PROTEIN STABILITY; PSYCHROPHILES;

BIOFUELS; CARBOHYDRATES; CLONING; ETHANOL; GENES; LAKES; LIQUID CHROMATOGRAPHY; METHANOL; PURIFICATION; RECOMBINANT PROTEINS; SODIUM CHLORIDE;

ENZYME ACTIVITY;

BETA GALACTOSIDASE; CARBOHYDRATE; COLD SHOCK PROTEIN;

ANTARCTICA; ARTICLE; COLD; GEL FILTRATION CHROMATOGRAPHY; GENE EXPRESSION; GENE SEQUENCE; HALORUBRUM LACUSPROFUNDI; HYDROPHOBIC INTERACTION CHROMATOGRAPHY; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; MOLECULAR CLONING; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; POLYMERASE CHAIN REACTION; PROTEIN ANALYSIS; PROTEIN PURIFICATION; SALINITY; SEQUENCE ANALYSIS;

ALCOHOLS; AMINO ACID SEQUENCE; ANTARCTIC REGIONS; BETA-GALACTOSIDASE; CLONING, MOLECULAR; COLD SHOCK PROTEINS AND PEPTIDES; GENOME; HALOBACTERIUM; HALORUBRUM; HYDROGEN-ION CONCENTRATION; MULTIGENE FAMILY; PROMOTER REGIONS, GENETIC; PROTEIN STABILITY; RECOMBINANT PROTEINS; SALTS; TEMPERATURE; WATER;

ARCHAEA; HALOBACTERIA; HALOBACTERIUM SP. NRC-1; HALORUBRUM LACUSPROFUNDI;

EID: 84872200697     PISSN: None     EISSN: 14726750     Source Type: Journal    
DOI: 10.1186/1472-6750-13-3     Document Type: Article

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