A novel metagenome-derived β-galactosidase: Gene cloning, overexpression, purification and characterization

Applied Microbiology and Biotechnology

Volumn 88, Issue 1, 2010, Pages 155-165

  Wang, Kui ^a   Li, Gang ^a   Yu, Shi Qin ^a   Zhang, Chen Ting ^a   Liu, Yu Huan ^a  

^a SUN YAT SEN UNIVERSITY   (China)

Author keywords

galactosidase; Cold adapted activity; Enzyme characterization; Gene cloning; Metagenome

Indexed keywords

COLD-ADAPTED ACTIVITY; ENZYME CHARACTERIZATION; GALACTOSIDASES; GENE CLONING; METAGENOMES;

AMINO ACIDS; CALCIUM; CLONING; ENZYMES; ORGANIC ACIDS; PURIFICATION; SODIUM; SUGARS;

ENZYME ACTIVITY;

4 NITROPHENYLGALACTOSIDE; AMINO ACID DERIVATIVE; BETA GALACTOSIDASE; CALCIUM ION; LACTOSE; POTASSIUM ION; SODIUM ION;

AMINO ACID; CLONE; ENZYME ACTIVITY; FOOD INDUSTRY; GENE; PROTEIN; SUBSTRATE;

ARTICLE; CONTROLLED STUDY; ENZYME ACTIVITY; GENE LIBRARY; GENE OVEREXPRESSION; GENE SEQUENCE; HYDROLYSIS; METAGENOME; MOLECULAR CLONING; MOLECULAR WEIGHT; NONHUMAN; NUCLEOTIDE SEQUENCE; PICHIA PASTORIS; PREDICTION; PROTEIN ISOLATION; PROTEIN PURIFICATION;

BETA-GALACTOSIDASE; CALCIUM; CLONING, MOLECULAR; CLUSTER ANALYSIS; COENZYMES; ENZYME STABILITY; GENE EXPRESSION; HYDROGEN-ION CONCENTRATION; LACTOSE; METAGENOME; MOLECULAR SEQUENCE DATA; MOLECULAR WEIGHT; NITROPHENYLGALACTOSIDES; PHYLOGENY; PICHIA; POTASSIUM; RECOMBINANT PROTEINS; SEQUENCE ANALYSIS, DNA; SEQUENCE HOMOLOGY, AMINO ACID; SODIUM; SOIL MICROBIOLOGY; TEMPERATURE;

PICHIA PASTORIS;

EID: 77955848869     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-010-2744-7     Document Type: Article

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