Discontinuous and continuous purification of single-chain antibody fragments using immobilized metal ion affinity chromatography

Journal of Biotechnology

Volumn 163, Issue 2, 2013, Pages 233-242

  Martínez Cristancho, Carlos Andrés ^a   David, Florian ^b   Franco Lara, Ezequiel ^b   Seidel Morgenstern, Andreas ^a,c  

^a MAX PLANCK INSTITUTE FOR DYNAMICS OF COMPLEX TECHNICAL SYSTEMS   (Germany)

^b TECHNICAL UNIVERSITY OF BRAUNSCHWEIG   (Germany)

^c OTTO VON GUERICKE UNIVERSITY   (Germany)

Author keywords

Adsorption equilibrium constants; Bacterial expression; Histidine tagged recombinant protein purification; Immobilized metal ion affinity chromatography; Simulated moving bed; Single chain Fragment variable antibody

Indexed keywords

ADSORPTION EQUILIBRIUM CONSTANTS; BACTERIAL EXPRESSION; IMMOBILIZED METAL ION AFFINITY CHROMATOGRAPHIES; RECOMBINANT PROTEIN PURIFICATION; SIMULATED MOVING BED;

ADSORPTION ISOTHERMS; AFFINITY CHROMATOGRAPHY; AMINO ACIDS; BACTERIOLOGY; BINARY MIXTURES; CELL CULTURE; EQUILIBRIUM CONSTANTS; LIQUID CHROMATOGRAPHY; PURIFICATION; RECOMBINANT PROTEINS;

ANTIBODIES;

SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY;

ADSORPTION; ARTICLE; BACILLUS MEGATERIUM; BACTERIUM CULTURE; BATCH PROCESS; CONTINUOUS PROCESS; CONTROLLED STUDY; DESORPTION; DRUG IMPURITY; DRUG PURIFICATION; ELUTION; IMMOBILIZED METAL AFFINITY CHROMATOGRAPHY; NONHUMAN; PH; POLYACRYLAMIDE GEL ELECTROPHORESIS; POROSITY; PRIORITY JOURNAL; PROCESS DESIGN; SIMULATION; SUPERNATANT;

BACILLUS MEGATERIUM; CHROMATOGRAPHY, AFFINITY; COMPUTER SIMULATION; ELECTROPHORESIS, POLYACRYLAMIDE GEL; HISTIDINE; HYDROGEN-ION CONCENTRATION; RECOMBINANT FUSION PROTEINS; SINGLE-CHAIN ANTIBODIES;

BACILLUS MEGATERIUM; BACTERIA (MICROORGANISMS);

EID: 84872196115     PISSN: 01681656     EISSN: 18734863     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2012.08.022     Document Type: Article

References (33)

1. Abel S., Mazzotti M., Morbidelli M. Solvent gradient operation of simulated moving beds. I. Adsorption equilibrium constants. Journal of Chromatography A 2002, 944:23-39.
2. Beltscheva D., Hugo P., Seidel-Morgenstern A. Linear two-step gradient counter-current chromatography analysis based on a recursive solution of an equilibrium stage model. Journal of Chromatography A 2003, 989:31-45.
3. Brereton H.M., Chamberlain D., Yang R., Tea M., McNeil S., Coster D., Williams K. Single chain antibody fragments for ocular use produced at high levels in a commercial wheat variety. Journal of Biotechnology 2007, 129:539-546.
4. Broughton, D.B., Gerhold, C.G., 1961. Continuous sorption process employing fixed beds of sorbent and moving inlets and outlets. U.S. Patent No. 2,985,589.
5. Buhlert K., Lehr M., Jungbauer A. Construction and development of a new single-column simulated moving bed system on the laboratory scale. Journal of Chromatography A 2009, 1216:8778-8786.
6. Carta G., Jungbauer A. Protein Chromatography: Process Development and Scale Up 2010, John Wiley and Sons.
7. Chaga G. Twenty-five years of immobilized metal ion affinity chromatography: past, present and future. Journal of Biochemical and Biophysical Methods 2001, 49:313-334.
8. Clemmitt R.H., Chase H.A. Facilitated downstream processing of a histidine-tagged protein from unclarified E. coli homogenates using immobilized metal affinity expanded-bed adsorption. Biotechnology and Bioengineering 2000, 67:206-216.
9. David F., Steinwand M., Hust M., Bohle K., Ross A., Dübel S., Franco-Lara E. Antibody production in Bacillus megaterium: strategies and physiological implications of scaling from microtiter plates to industrial bioreactors. Biotechnology Journal 2011, 6:1516-1531.
10. David F., Westphal R., Bunk B., Jahn D., Franco-Lara E. Optimization of antibody fragment production in Bacillus megaterium: the role of metal ions on protein secretion. Journal of Biotechnology 2010, 150:115-124.
11. Deutscher M.P. Guide to Protein Purification: Methods in Enzymology 1990, vol. 182. Academic Press.
12. Essen L., Skerra A. Single-step purification of a bacterially expressed antibody Fv fragment by immobilized metal affinity chromatography in the presence of betaine. Journal of Chromatography A 1993, 657:55-61.
13. Gottschalk U. Process Scale Purification of Antibodies 2009, John Wiley and Sons.
14. Gueorguieva L., Palani S., Rinas U., Jayaraman G., Seidel-Morgenstern A. Recombinant protein purification using gradient-assisted simulated moving bed hydrophobic interaction chromatography. Part II: Process design and experimental validation. Journal of Chromatography A 2011, 1218:6402-6411.
15. Guiochon G., Felinger A., Shirazi D.G., Katti A.M. Fundamentals of Preparative and Nonlinear Chromatography 2006, Elsevier.
16. Hochuli E., Bannwarth W., Döbeli H., Gentz R., Stüber D. Genetic approach to facilitate purification of recombinant proteins with a novel metal chelate adsorbent. Nature Biotechnology 1988, 6:1321-1325.
17. Hust M., Dübel S. Mating antibody phage display with proteomics. Trends in Biotechnology 2004, 22:8-14.
18. Jordan E., Hust M., Roth A., Biedendieck R., Schirrmann T., Jahn D., Dübel S. Production of recombinant antibody fragments in Bacillus Megaterium. Microbial Cell Factories 2007, 6:2.
19. Kessler L., Gueorguieva L., Rinas U., Seidel-Morgenstern A. Step gradients in 3-zone simulated moving bed chromatography: application to the purification of antibodies and bone morphogenetic protein-2. Journal of Chromatography A 2007, 1176:69-78.
20. 2+-NTA surfaces. Journal of Molecular Recognition 2009, 22:270-279.
21. Krättli M., Ströhlein G., Aumann L., Müller-Späth T., Morbidelli M. Closed loop control of the multi-column solvent gradient purification process. Journal of Chromatography A 2011, 1218:9028-9036.
22. Laemmli U. Cleavage of structural proteins during the assembly of the head of bacteriophage t4. Nature 1970, 227:680-685.
23. Mazzotti M., Storti G., Morbidelli M. Optimal operation of simulated moving bed units for nonlinear chromatographic separations. Journal of Chromatography A 1997, 769:3-24.
24. Müller-Späth T., Aumann L., Melter L., Ströhlein G., Morbidelli M. Chromatographic separation of three monoclonal antibody variants using multicolumn countercurrent solvent gradient purification (MCSGP). Biotechnology and Bioengineering 2008, 100:1166-1177.
25. Ottens M., Houwing J., Van Hateren S.H., Van Baalen T., Van der Wielen L.A.M. Multi-component fractionation in SMB chromatography for the purification of active fractions from protein hydrolysates. Food and Bioproducts Processing 2006, 84:59-71.
26. Palani S., Gueorguieva L., Rinas U., Seidel-Morgenstern A., Jayaraman G. Recombinant protein purification using gradient-assisted simulated moving bed hydrophobic interaction chromatography. Part I: Selection of chromatographic system and estimation of adsorption isotherms. Journal of Chromatography A 2011, 1218:6396-6401.
27. Porath J. Immobilized metal ion affinity chromatography. Protein Expression and Purification 1992, 3:263-281.
28. Porath J., Carlsson J., Olsson I., Belfrage G. Metal chelate affinity chromatography, a new approach to protein fractionation. Nature 1975, 258:598-599.
29. Scott M., Clarke N. Next generation antibody therapeutics: antibody fragments, dual-targeting strategies, and beyond. European Pharmaceutical Review 2009, 5:22-29.
30. Skerra A., Pfitzinger I., Plückthun A. The functional expression of antibody Fv fragments in E. coli: improved vectors and a generally applicable purification technique. Nature Biotechnology 1991, 9:273-278.
31. Storti G., Mazzotti M., Morbidelli M., Carrà S. Robust design of binary countercurrent adsorption separation processes. AIChE Journal 1993, 39:471-492.
32. Weisser N.E., Hall J.C. Applications of single-chain variable fragment antibodies in therapeutics and diagnostics. Biotechnology Advances 2009, 27:502-520.
33. Yang Y., Malten M., Grote A., Jahn D., Deckwer W.D. Codon optimized Thermobifida fusca hydrolase secreted by Bacillus megaterium. Biotechnology and Bioengineering 2007, 96:780-794.