Codon optimized Thermobifida fusca hydrolase secreted by Bacillus megaterium

Biotechnology and Bioengineering

Volumn 96, Issue 4, 2007, Pages 780-794

  Yang, Yang ^a   Malten, Marco ^a   Grote, Andreas ^a   Jahn, Dieter ^a   Deckwer, Wolf Dieter ^a  

^a TECHNICAL UNIVERSITY OF BRAUNSCHWEIG   (Germany)

Author keywords

Bacillus megaterium; Codon usage; Expression; Secretion; Thermobifida fusca hydrolase

Indexed keywords

BACTERIA; BIOREACTORS; CLONING; GLUCOSE; VOLUMETRIC ANALYSIS; XYLOSE;

BACILLUS MEGATERIUM; CODON USAGE; EXPRESSIONS; SECRETION; THERMOBIFIDA FUSCA HYDROLASE;

ENZYMES;

ACID LIPASE; BACTERIAL ENZYME; HYDROLASE; POLYHISTIDINE TAG; XYLOSE;

AFFINITY CHROMATOGRAPHY; ARTICLE; BACILLUS MEGATERIUM; BACTERIAL CELL; BATCH REACTOR; CELL DENSITY; CODON; CONTROLLED STUDY; DNA SEQUENCE; FED BATCH CULTURE; FED BATCH REACTOR; GENE EXPRESSION; NONHUMAN; OPEN READING FRAME; THERMOMONOSPORA;

ACTINOMYCETALES; BACILLUS MEGATERIUM; CODON, INITIATOR; GENE EXPRESSION REGULATION, BACTERIAL; GENETIC ENHANCEMENT; HYDROLASES; INDUSTRIAL MICROBIOLOGY; RECOMBINANT PROTEINS;

BACILLUS MEGATERIUM; THERMOBIFIDA FUSCA;

EID: 33947178303     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.21167     Document Type: Article

References (31)

1. Barg H, Malten M, Jahn M, Jahn D. 2005. Protein and vitamin production in Bacillus megaterium. In: Barredo JL, editor. Microbial processes and products. Totowa: Humana Press Inc. pp 165-184.
2. Biedendieck R, Malten M, Yang Y, Deckwer W-D, Jahn D. 2006. Plasmid system for the intracellular production and purification of affinity-tagged proteins in Bacillus megaterium. Biotechnol Bioeng (in press).
3. Deckwer W-D, Müller RJ, Van den Heuvel J, Kleeberg I. 2001. Enzyme which cleaves ester groups and which is derived from Thermomonospora fusca. GBF patent W001123581A1.
4. Dresler K, van den Heuvel J, Muller RJ, Deckwer W-D. 2006. Production of a recombinant polyester-cleaving hydrolase from Thermobifida fusca in Escherichia coli. Bioprocess Biosyst Eng 29:169-183.
5. Gottschalk G. 1986. Bacterial metabolism. New York: Springer Verlag.
6. Gouda MK, Kleeberg I, van den Heuvel J, Muller RJ, Deckwer W-D. 2002. Production of a polyester degrading extracellular hydrolase from Thermomonospora fusca. Biotechnol Prog 18:927-934.
7. Grote A, Hiller K, Scheer M, Munch R, Nortemann B, Hempel DC, Jahn D. 2005. JCat: A novel tool to adapt codon usage of a target gene to its potential expression host. Nucleic Acids Res 33:526-531.
8. Hefti MH, Van Vugt-Van der Toorn CJ, Dixon R, Vervoort J. 2001. A novel purification method for histidine-tagged proteins containing a thrombin cleavage site. Anal Biochem 295:180-185.
9. Herbort M, Klein M, Manting EH, Driessen AJ, Freudl R. 1999. Temporal expression of the Bacillus subtilis secA gene, encoding a central component of the preprotein translocase. J Bacteriol 181:493-500.
10. Hollmann R, Deckwer W-D. 2004. Pyruvate formation and suppression in recombinant Bacillus megaterium cultivation. J Biotechnol 111:89-96.
11. Jeong KJ, Park IY, Kim MS, Kim SC. 1998. High-level expression of an endoxylanase gene from Bacillus sp. in Bacillus subtilis DB104 for the production of xylobiose from xylan. Appl Microbiol Biotechnol 50:113-118.
12. Kleeberg I, Welzel K, Vandenheuvel J, Müller RJ, Deckwer W-D. 2005. Characterization of a new extracellular hydrolase from Thermobifida fusca degrading aliphatic-aromatic copolyesters. Biomacromolecules 6:262-270.
13. Korz DJ. 1993. Entwicklung von Prozeßstrategien zur Kultivierung von Escherichia coli zu hohen Zelldichten. Munich: Technical University of Munich.
14. Leloup L, Le Saux J, Petit-Glatron MF, Chambert R. 1999. Kinetics of the secretion of Bacillus subtilis levanase overproduced during the exponential phase of growth. Microbiology 145:613-619.
15. Makrides SC. 1996. Strategies for achieving high-level expression of genes in Escherichia coli. Microbiol Rev 60:512-538.
16. Malten M, Hollmann R, Deckwer W-D, Jahn D. 2005a. Production and secretion of recombinant Leuconostoc mesenteroides dextransucrase DsrS in Bacillus megaterium. Biotechnol Bioeng 89:206-218.
17. Malten M, Nahrstedt H, Meinhardt F, Jahn D. 2005b. Coexpression of the type I signal peptidase gene sipM increases recombinant protein production and export in Bacillus megaterium MS941. Biotechnol Bioeng 91:616-621.
18. Malten M, Biedendieck R, Gamer M, Drews AC, Stammen S, Buchholz K, Dijkhuizen L, Jahn D. 2006. A Bacillus megaterium plasmid system for the production, export, and one-step purification of affinity-tagged heterologous levansucrase from growth medium. Appl Environ Microbiol 72:1677-1679.
19. Mason JM, Fajardo-Cavazos P, Setlow P. 1988. Levels of mRNAs which code for small, acid-soluble spore proteins and their LacZ gene fusions in sporulating cells of Bacillus subtilis. Nucleic Acids Res 16:6567-6583.
20. Müller RJ, Schrader H, Profe J, Dresler K, Deckwer W-D. 2005. Enzymatic degradation of poly(ethylene terephthalate): Rapid hydrolyse using a hydrolase from T. fusca. Macromol Rapid Commun 26:1400-1405.
21. Rygus T, Hillen W. 1991. Inducible high-level expression of heterologous genes in Bacillus megaterium using the regulatory elements of the xylose-utilization operon. Appl Microbiol Biotechnol 35:594-599.
22. Rygus T, Hillen W. 1992. Catabolite repression of the xyl operon in Bacillus megaterium. J Bacteriol 174:3049-3055.
23. Rygus T, Scheler A, Allmansberger R, Hillen W. 1991. Molecular cloning, structure, promoters and regulatory elements for transcription of the Bacillus megaterium encoded regulon for xylose utilization. Arch Microbiol 155:535-542.
24. Sambrook J, Russell D. 2001. Molecular cloning: A laboratory manual. Woodbury, USA: Cold Spring Harbor Laboratory Press. 999 p.
25. Sarath G, De la Motte RS, Wagner FW. 1989. Protease assay methods. In: Beynon RJ, Bond JS, editors. Proteolytic enzymes. New York: IRL Press. pp 25-55.
26. Schallmey M, Singh A, Ward OP. 2004. Developments in the use of Bacillus species for industrial production. Can J Microbiol 50:1-17.
27. Schmidt-Dannert C, Sztajer H, Stocklein W, Menge U, Schmid RD. 1994. Screening, purification and properties of a thermophilic lipase from Bacillus thermocatenulatus. Biochim Biophys Acta 1214:43-53.
28. Sharipova MR. 2002. Late stages of protein secretion in bacilli. Biochemistry (Mosc) 67:1207-1216.
29. Talarico LA, Gil MA, Yomano LP, Ingram LO, Maupin-Furlow JA. 2005. Construction and expression of an ethanol production operon in Gram-positive bacteria10.1099/mic.0.28375-0. Microbiology 151:4023-4031.
30. Vary PS. 1994. Prime time for Bacillus megatenum. Microbiology 140:1001-1013.
31. Wittchen KD, Meinhardt F. 1995. Inactivation of the major extracellular protease from Bacillus megaterium DSM319 by gene replacement. Appl Microbiol Biotechnol 42:871-877.