Amylin receptor phenotypes derived from human calcitonin receptor/RAMP coexpression exhibit pharmacological differences dependent on receptor isoform and host cell environment

Journal of Pharmacology and Experimental Therapeutics

Volumn 294, Issue 1, 2000, Pages 61-72

  Tilakaratne, Nanda ^b   Christopoulos, George ^b   Zumpe, Emma T ^b   Foord, Steven M ^a   Sexton, Patrick M ^b  

^a GLAXOSMITHKLINE   (United Kingdom)

^b NONE

Author keywords

[No Author keywords available]

Indexed keywords

AMYLIN; CALCITONIN; CALCITONIN GENE RELATED PEPTIDE; CALCITONIN RECEPTOR; G PROTEIN COUPLED RECEPTOR; HORMONE RECEPTOR; SALCATONIN;

ANIMAL CELL; ARTICLE; CELL STRAIN COS1; CHO CELL; CONTROLLED STUDY; GENE EXPRESSION; GENETIC TRANSFECTION; HORMONE RECEPTOR INTERACTION; HOST CELL; NONHUMAN; PHENOTYPE; PRIORITY JOURNAL; PROTEIN FAMILY; RECEPTOR AFFINITY; STOICHIOMETRY;

AMYLOID; ANIMALS; CHO CELLS; COS CELLS; CRICETINAE; HUMANS; INTRACELLULAR SIGNALING PEPTIDES AND PROTEINS; MEMBRANE PROTEINS; PHENOTYPE; PROTEIN ISOFORMS; RECEPTORS, CALCITONIN; RECEPTORS, PEPTIDE; TRANSFECTION;

EID: 0033932339     PISSN: 00223565     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (22)

1. Beaumont K, Kenney MA, Young AA and Rink TJ (1993) High affinity amylin binding sites in rat brain. Mol Pharmacol 44:493-497.
2. Buhlmann N, Leuthauser K, Muff R, Fischer JA and Born W (1999) A receptor activity modifying protein (RAMP)2-dependent adrenomedullin receptor is a calcitonin gene-related peptide receptor when coexpressed with human RAMP1. Endocrinology 140:2883-2890.
3. Chai SY, Christopoulos G, Cooper ME and Sexton PM (1997) Characterization of binding sites for amylin, calcitonin, and CGRP in primate kidney. Am J Physiol 274:F51-F62.
4. Chomczynski P and Sacchi N (1987) Single-step method of RNA isolation by acid guanidium thiocyanate-pheol-chloroform extraction. Anal Biochem 162:156-159.
5. Christopoulos G, Perry KJ, Morfis M, Tilakaratne N, Gao Y, Fraser NJ, Main MJ, Foord SM and Sexton PM (1999) Multiple amylin receptors arise from RAMP interaction with the calcitonin receptor gene product. Mol Pharmacol 56:235-242.
6. Cooper ME and Wookey PJ (1997) Amylin: Its role in the kidney. Nephrol Dial Transplant 12:8-10.
7. Fraser NJ, Wise A, Brown J, McLatchie LM, Main MJ and Foord SM (1999) The amino terminus of receptor activity modifying proteins is a critical determinant of glycosylation state and ligand binding of calcitonin receptor-like receptor. Mol Pharmacol 55:1054-1059.
8. Ishihara T, Nakamura S, Kaziro Y, Takahashi T, Takahashi K and Nagata S (1991) Molecular cloning and expression of a cDNA encoding the secretin receptor. EMBO J 10:1635-1641.
9. Kenakin T (1997) Differences between natural and recombinant G protein-coupled receptor systems with varying receptor/G protein stoichiometry. Trends Pharmacol Sci 18:456-464.
10. Kreutter DK, Orena SJ, Torchia AJ, Contillo LG, Andrews GC and Stevenson RW (1993) Amylin and CGRP induce insulin resistance via a receptor distinct from cAMP-coupled CGRP receptor. Am J Physiol 264:E606-E613.
11. Kuestner RE, Elrod RD, Grant FJ, Hagen FS, Kuijper JL, Mathewes SL, O'Hara PJ, Sheppard PO, Stroop SD, Thompson DL, Whitmore TE, Findlay D, Houssami S, Sexton PM and Moore E (1994) Cloning and characterization of an abundant subtype of the human calcitonin receptor. Mol Pharmacol 46:246-255.
12. Leighton B and Cooper GJS (1988) Pancreatic amylin and calcitonin gene-related peptide cause resistance to insulin in skeletal muscle in vitro. Nature (Lond) 335:632-635.
13. McLatchie LM, Fraser NJ, Main MJ, Wise A, Brown J, Thompson N, Solari R, Lee MG and Foord SM (1998) RAMPS regulate the transport and ligand specificity of the calcitonin receptor-like receptor. Nature (Lond) 393:333-339.
14. Moore EE, Kuestner RE, Stroop SD, Grant FJ, Matthewes SL, Brady CL, Sexton PM and Findlay DM (1995) Functionally different isoforms of the human calcitonin receptor result from alternative splicing of the gene transcript. Mol Endocrinol 9:959-968.
15. Muff R, Buhlmann N, Fischer JA and Born W (1999) An amylin receptor is revealed following co-transfection of a calcitonin receptor with receptor activity modifying proteins-1 or -3. Endocrinology 140:2924-2927.
16. Naro F, Perez M, Migliaccio S, Galson DL, Orcel P, Teti A and Goldring SR (1998) Phospholipase D- and protein kinase C isoenzyme-dependent signal transduction pathways activated by the calcitonin receptor. Endocrinology 139:3241-3248.
17. Nussenzveig DR, Thaw CN and Geshengorn MC (1994) Inhibition of inositol phosphate second messenger formation by intracellular loop one of a human calcitonin receptor. Expression and mutational analysis of synthetic receptor genes. J Biol Chem 269:28123-28129.
18. Rossowski WJ, Jiang NY and Coy DH (1997) Adrenomedullin, amylin, calcitonin gene-related peptide and their fragments are potent inhibitors of gastric acid secretion in rats. Eur J Pharmacol 336:51-63.
19. Sexton PM, Paxinos G, Kenney MA, Wookey PJ and Beaumont K (1994) In vitro autoradiographic localization of amylin binding sites in rat brain. Neuroscience 62:553-567.
20. Sexton PM and Perry KJ (1996) Amylin receptors in the central nervous system. Recent Res Devel Neurochem 1:157-166.
21. Wookey PJ, Tikellis C, Du H-C, Qin HF, Sexton PM and Cooper ME (1996) Amylin binding in rat renal cortex, stimulation of adenylyl cyclase, and activation of plasma renin. Am J Physiol 270:F289-F294.
22. Zumpe ET, Tilakaratne N, Fraser NJ, Christopoulos G, Foord SM and Sexton PM (2000) Multiple RAMP domains are required for generation of amylin receptor phenotype from the calcitonin receptor gene product. Biochem Biophys Res Commun 267:368-372.