Technical brief: Isolation of total DNA from postmortem human eye tissues and quality comparison between iris and retina

Molecular Vision

Volumn 18, Issue , 2012, Pages 3049-3056

  Wang, Jay Ching Chieh ^a   Wang, Aikun ^a   Gao, Jiangyuan ^a   Cao, Sijia ^a   Samad, Idris ^a   Zhang, Dean ^a   Ritland, Carol ^a   Cui, Jing Z ^a   Matsubara, Joanne Aiko ^a  

^a UNIVERSITY OF BRITISH COLUMBIA   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

BOVINE SERUM ALBUMIN; CHLOROFORM; COMPLEMENT FACTOR H; ISOPENTYL ALCOHOL; PHENOL;

ARTICLE; AUTOPSY; COMPLEMENT FACTOR H GENE; CONTROLLED STUDY; DNA DETERMINATION; DNA ISOLATION; DNA PURIFICATION; GEL ELECTROPHORESIS; GENE AMPLIFICATION; GENOMICS; GENOTYPING TECHNIQUE; HUMAN; HUMAN TISSUE; IRIS; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; QUALITATIVE ANALYSIS; QUANTITATIVE ANALYSIS; RETINA; SPECTROPHOTOMETRY; TISSUE DISTRIBUTION;

ADULT; AGED; ANIMALS; AUTOPSY; BASE SEQUENCE; CATTLE; COMPLEMENT FACTOR H; DNA; ELECTROPHORESIS, AGAR GEL; GENOTYPE; GENOTYPING TECHNIQUES; HUMANS; IRIS; MIDDLE AGED; MOLECULAR SEQUENCE DATA; POLYMERASE CHAIN REACTION; POLYMORPHISM, SINGLE NUCLEOTIDE; RETINA; SERUM ALBUMIN, BOVINE;

EID: 84871884717     PISSN: None     EISSN: 10900535     Source Type: Journal    
DOI: None     Document Type: Article

References (19)

1. Sheffield VC, Stone EM. Genomics and the eye. N Engl J Med 2011; 364:1932-42. [PMID: 21591945].
2. Hageman GS, Anderson DH, Johnson LV, Hancox LS, Taiber AJ, Hardisty LI, Hageman JL, Stockman HA, Borchardt JD, Gehrs KM, Smith RJH, Silvestri G, Russell SR, Klaver CCW, Barbazetto I, Chang S, Yannuzzi LA, Barile GR, Merriam JC, Smith RT, Olsh AK, Bergeron J, Zernant J, Merriam JE, Gold B, Dean M, Allikmets R. A common haplotype in the complement regulatory gene factor H (HF1/CFH) predisposes individuals to age-related macular degeneration. Proc Natl Acad Sci USA 2005; 102:7227-32. [PMID: 15870199].
3. Haines JL, Hauser MA, Schmidt S, Scott WK, Olson LM, Gallins P, Spencer KL, Kwan SY, Noureddine M, Gilbert JR, Schnetz-Boutaud N, Agarwal A, Postel EA, Pericak-Vance MA. Complement factor H variant increases the risk of age-related macular degeneration. Science 2005; 308:419-21. [PMID: 15761120].
4. Edwards AO, Ritter R, Abel KJ, Manning A, Panhuysen C, Farrer LA. Complement factor H polymorphism and age-related macular degeneration. Science 2005; 308:421-4. [PMID: 15761121].
5. Klein RJ, Zeiss C, Chew EY, Tsai JY, Sackler RS, Haynes C, Henning AK, SanGiovanni JP, Mane SM, Mayne ST, Bracken MB, Ferris FL, Ott J, Barnstable C, Hoh J. Complement factor H polymorphism in age-related macular degeneration. Science 2005; 308:385-9. [PMID: 15761122].
6. Mullins RF, Dewald AD, Streb LM, Wang K, Kuehn MH, Stone EM. Elevated membrane attack complex in human choroid with high risk complement factor H genotypes. Exp Eye Res 2011; 93:565-7. [PMID: 21729696].
7. Smailhodzic D, Klaver CC, Klevering BJ, Boon CJ, Groenewoud JM, Kirchhof B, Daha MR, den Hollander AI, Hoyng CB. Risk alleles in CFH and ARMS2 are independently associated with systemic complement activation in age-related macular degeneration. Ophthalmology 2012; 119:339-46. [PMID: 22133792].
8. Sivaprasad S, Adewoyin T, Bailey TA, Dandekar SS, Jenkins S, Webster AR, Chong NV. Estimation of systemic complement C3 activity in age-related macular degeneration. Arch Ophthalmol 2007; 125:515-9. [PMID: 17420372].
9. Perdigon HB, Calacal GC, Seng KLC, Halos SC, de Ungria MCA. Evaluation and in-house validation of five DNA extraction methods for PCR-based STR analysis of bloodstained denims. Science Dilimann. 2004; 16:37-48.
10. Prośniak A, Gloc E, Berent J, Babol-Pokora K, Jacewicz R, Szram S. Estimating the efficiency of DNA isolation methods in semen, blood and saliva stains using the QuantiBlot system. Arch Med Sadowej Kryminol 2006; 56:19-23. [PMID: 16708611].
11. Dissing J, Sondervang A, Lund S. Exploring the limits for the survival of DNA in blood stains. J Forensic Leg Med 2010; 17:392-6. [PMID: 20851360].
12. Lachaud L, Chabbert E, Dubessay P, Reynes J, Lamothe J, Bastien P. Comparison of various sample preparation methods for PCR diagnosis of visceral leishmaniasis using peripheral blood. J Clin Microbiol 2001; 39:613-7. [PMID: 11158116].
13. Clements DN, Wood S, Carter SD, Ollier WE. Assessment of the quality and quantity of genomic DNA recovered from canine blood samples by three different extraction methods. Res Vet Sci 2008; 85:74-9. [PMID: 18031774].
14. Strauss WM. Preparation of genomic DNA from mammalian tissue. Curr Protoc Neurosci. Appendix 2001; 1:Appendix1H-.
15. Giambernardi TA, Rodeck U, Klebe RJ. Bovine serum albumin reverses inhibition of RT-PCR by melanin. Biotechniques 1998; 25:564-6. [PMID: 9793633].
16. Tang W, David FB, Wilson MM, Barwick BG, Leyland-Jones BR, Bouzyk MM. DNA extraction from formalin-fixed, paraffin-embedded tissue. Cold Spring Harb Protoc. 2009; 2009:prot5138-[PMID: 20147068].
17. Gilbert MT, Haselkorn T, Bunce M, Sanchez JJ, Lucas SB, Jewell LD, Van Marck E, Worobey M. The isolation of nucleic acids from fixed, paraffin-embedded tissues-which methods are useful when? PLoS ONE 2007; 2:e537-[PMID: 17579711].
18. Eagle RC. Eye pathology-an atlas and text. 2nd ed ed. Lippincott Williams & Wilkins; 2011.
19. Eckhart L, Bach J, Ban J, Tschachler E. Melanin binds reversibly to thermostable DNA polymerase and inhibits its activity. Biochem Biophys Res Commun 2000; 271:726-30. [PMID: 10814530].