Bovine serum albumin reverses inhibition of RT-PCR by melanin

BioTechniques

Volumn 25, Issue 4, 1998, Pages 564-566

  Giambernardi, Troy A ^a   Rodeck, Ulrich ^a,b   Klebe, Robert J ^a  

^a UNIVERSITY OF TEXAS HEALTH SCIENCE CENTER AT SAN ANTONIO   (United States)

^b THOMAS JEFFERSON UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BOVINE SERUM ALBUMIN; MELANIN;

ARTICLE; COMPETITIVE INHIBITION; GENETIC POLYMORPHISM; INHIBITION KINETICS; MELANOCYTE; MELANOGENESIS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION;

BOVINAE;

EID: 0031688927     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/98254bm03     Document Type: Article

References (5)

1. Chen, D., V.L. Magnuson, B. Steffensen and R.J. Klebe. 1993. Use of stock solutions to simplify mRNA quantitation by reverse transcription-polymerase chain reaction (RT-PCR) assays. PCR Methods Appl. 2:351-353.
2. Giambernardi, T.A., G.M. Grant, V.M. Maher, J.J. McCormick, G.P. Taylor and R.J. Klebe. 1998. Overview of matrix metalloproteinase expression in transformed cells. Matrix Biol. 16:483-496.
3. Hill, H.Z., C. Huselton, B. Pilas and G.J. Hill. 1987. Ability of melanins to protect against the radiolysis of thymine and thymidine. Pigment Cell Res. 1:81-86.
4. Kreader, C.A. 1996. Relief of amplification inhibition in PCR with bovine serum albumin or T4 gene 32 protein. Appl. Environ. Microbiol. 62:1102-1106.
5. Yoshii, T., K. Ramura, T. Taniguchi and K.I.I. Akiyama. 1993. Water-soluble eumelanin as a PCR-inhibitor and a simple method for its removal. Jpn. J. Leg. Med. 47:323-329.