DNA extraction from formalin-fixed, paraffin-embedded tissue

Cold Spring Harbor Protocols

Volumn 4, Issue 2, 2009, Pages

  Tang, Weining ^a   David, Freedom B ^a   Wilson, Malania M ^a   Barwick, Benjamin G ^a   Leyland Jones, Brian R ^a   Bouzyk, Mark M ^a,b  

^a EMORY UNIVERSITY   (United States)

^b EMORY UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DNA; FORMALDEHYDE; PARAFFIN; PROTEINASE; REAGENT; RIBONUCLEASE;

ARTICLE; DNA EXTRACTION; DNA ISOLATION; DNA PURIFICATION; ENZYME DEGRADATION; QUANTITATIVE ANALYSIS; TISSUE FIXATION; TISSUE PRESERVATION;

EID: 65749112359     PISSN: None     EISSN: 15596095     Source Type: Journal    
DOI: 10.1101/pdb.prot5138     Document Type: Article

References (6)

1. Abramovitz, M., Ordanic-Kodani, M., Wang, Y., Li, Z., Catzavelos, C., Bouzyk, M., Sledge Jr, G.W., Moreno, C.S., and Leyland-Jones, B. 2008. Optimization of RNA extraction from FFPE tissues for expression profiling in the DASL assay. Biotechniques 44: 417-423.
2. Barbas III, C.F., Burton, D.R., Scott, J.K., and Silverman, G.J. 2007. Quantitation of DNA and RNA. Cold Spring Harb. Protoc. doi: 10.1101/pdb.ip47.
3. Blow, N. 2007. Tissue preparation: Tissue issues. Nature 448: 959-963.
4. Lehmann, U. and Kreipe, H. 2001. Real-time PCR analysis of DNA and RNA extracted from formalin-fixed and paraffin-embedded biopsies. Methods 25: 409-418.
5. Li, J., Smyth, P., Flavin, R., Cahill, S., Denning, K., Aherne, S., Guenther, S.M., O'Leary, J.J., and Sheils, O. 2007. Comparison of miRNA expression patterns using total RNA extracted from matched samples of formalin-fixed paraffin-embedded (FFPE) cells and snap frozen cells. BMC Biotechnol. 29: 36. doi: 10.1186/1472-6750-7-36.
6. Little, S.E., Vuononvirta, R., Reis-Filho, J.S., Natrajan, R., Iravani, M., Fenwick, K., Mackay, A., Ashworth, A., Pritchard-Jones, K., and Jones, C. 2006. Array CGH using whole genome amplification of fresh-frozen and formalin-fixed, paraffin-embedded tumor DNA. Genomics 87: 298-306.