Identification of cargo proteins specific for the nucleocytoplasmic transport carrier transportin by combination of an in vitro transport system and stable isotope labeling by amino acids in cell culture (SILAC)-based quantitative proteomics

Molecular and Cellular Proteomics

Volumn 12, Issue 1, 2013, Pages 145-157

  Kimura, Makoto ^a   Kose, Shingo ^a   Okumura, Nobuaki ^b   Imai, Kenichiro ^c   Furuta, Maiko ^a   Sakiyama, Noriyuki ^c   Tomii, Kentaro ^d   Horton, Paul ^c   Takao, Toshifumi ^b   Imamoto, Naoko ^a  

^a RIKEN   (Japan)

^b OSAKA UNIVERSITY   (Japan)

^c NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY AIST   (Japan)

^d JAPAN SOCIETY FOR THE PROMOTION OF SCIENCE   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

ARGININE; KARYOPHERIN BETA; LYSINE; NUCLEOCYTOPLASMIC TRANSPORT PROTEIN; STABLE ISOTOPE; TRANSPORTIN; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ANALYTIC METHOD; ARTICLE; BINDING ASSAY; CELLULAR DISTRIBUTION; CULTURE MEDIUM; HIGH THROUGHPUT SCREENING; HUMAN; IN VITRO STUDY; ISOTOPE LABELING; LIQUID CHROMATOGRAPHY; NUCLEOCYTOPLASMIC TRANSPORT; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DEPLETION; PROTEIN ISOLATION; PROTEIN LOCALIZATION; PROTEIN RNA BINDING; PROTEIN TRANSPORT; PROTEOMICS; QUANTITATIVE ASSAY; RNA TRANSPORT; STABLE ISOTOPE LABELING BY AMINO ACIDS IN CELL CULTURE; TANDEM MASS SPECTROMETRY; TRANSPORT KINETICS;

ACTIVE TRANSPORT, CELL NUCLEUS; AMINO ACID SEQUENCE; AMINO ACIDS; BETA KARYOPHERINS; CELL MEMBRANE; CHROMATOGRAPHY, LIQUID; HUMANS; ISOTOPE LABELING; NUCLEAR ENVELOPE; NUCLEAR LOCALIZATION SIGNALS; PROTEIN BINDING; PROTEIN STRUCTURE, TERTIARY; PROTEIN TRANSPORT; PROTEOMICS; TANDEM MASS SPECTROMETRY;

EID: 84871881617     PISSN: 15359476     EISSN: 15359484     Source Type: Journal    
DOI: 10.1074/mcp.M112.019414     Document Type: Article

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