Combinatorial mutagenesis and selection of improved signal sequences and their application for high-level production of translocated heterologous proteins in Escherichia coli

Applied and Environmental Microbiology

Volumn 79, Issue 2, 2013, Pages 559-568

  Heggeset, Tonje Marita Bjerkan ^a   Kucharova, Veronika ^b   Naerdal, Ingemar ^a   Valla, Svein ^b   Sletta, Hâvard ^a   Ellingsen, Trond Erling ^a   Brautaset, Trygve ^a  

^a SINTEF MATERIALS AND CHEMISTRY   (Norway)

^b NORWEGIAN UNIVERSITY OF SCIENCE AND TECHNOLOGY   (Norway)

Author keywords

[No Author keywords available]

Indexed keywords

ALKALINE PHOSPHATASE; BIOINFORMATICS ANALYSIS; COMPARATIVE STUDIES; E. COLI; EXPRESSION LEVELS; HETEROLOGOUS PROTEIN PRODUCTION; HETEROLOGOUS PROTEINS; HIGH-CELL-DENSITY CULTIVATIONS; HIGH-LEVEL PRODUCTION; HOST CELLS; MUTANT LIBRARIES; NOVEL DESIGN; PRODUCTION LEVEL; REPORTER GENE; SIGNAL PEPTIDE; SIGNAL SEQUENCE; TOLERANCE LEVELS;

BIOINFORMATICS; CLONING; ENCODING (SYMBOLS); ESCHERICHIA COLI; GENE ENCODING; OLIGONUCLEOTIDES; PHOSPHATASES;

PROTEINS;

BETA LACTAMASE; MUTANT PROTEIN; RECOMBINANT PROTEIN; SIGNAL PEPTIDE;

COLIFORM BACTERIUM; ENZYME ACTIVITY; GENE EXPRESSION; MUTAGENICITY; MUTATION; PROTEIN; SELECTION; SIGNAL; TRANSLOCATION;

ARTICLE; ESCHERICHIA COLI; GENETIC SELECTION; GENETICS; METABOLISM; METHODOLOGY; MUTAGENESIS; PENICILLIN RESISTANCE; PROTEIN ENGINEERING; SECRETION (PROCESS);

AMPICILLIN RESISTANCE; BETA-LACTAMASES; ESCHERICHIA COLI; MUTAGENESIS; MUTANT PROTEINS; PROTEIN ENGINEERING; PROTEIN SORTING SIGNALS; RECOMBINANT PROTEINS; SELECTION, GENETIC;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 84871868618     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.02407-12     Document Type: Article

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