Optimization of a heterologous signal peptide by site-directed mutagenesis for improved secretion of recombinant proteins in Escherichia coli

Journal of Molecular Microbiology and Biotechnology

Volumn 22, Issue 1, 2012, Pages 48-58

  Jonet, Mohd Anuar ^a   Mahadi, Nor Muhammad ^b   Murad, Abdul Munir Abdul ^c   Rabu, Amir ^c   Bakar, Farah Diba Abu ^c   Rahim, Raha Abdul ^d   Low, Kheng Oon ^a   Illias, Rosli Md ^a  

^a UNIVERSITI TEKNOLOGI MALAYSIA   (Malaysia)

^b Kemeneterian Sains Teknologi dan Inovasi   (Malaysia)

^c UNIVERSITI KEBANGSAAN MALAYSIA   (Malaysia)

^d UNIVERSITY PUTRA MALAYSIA   (Malaysia)

Author keywords

Cyclodextrin glucanotransferase; Escherichia coli; Heterologous signal peptide; Secretion; Site directed mutagenesis

Indexed keywords

CYCLOMALTODEXTRIN GLUCANOTRANSFERASE; GLYCINE; ISOLEUCINE; MANNOSIDASE; MUTANT PROTEIN; RECOMBINANT PROTEIN; SIGNAL PEPTIDE;

AMINO ACID SUBSTITUTION; ARTICLE; BACILLUS; BACTERIAL CELL; CELL GROWTH; CELL INCLUSION; CELL VIABILITY; CONTROLLED STUDY; CYTOLYSIS; CYTOPLASM; ESCHERICHIA COLI; EXTRACELLULAR SPACE; HYDROPHOBICITY; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN MOTIF; PROTEIN SECRETION; PROTEIN STRUCTURE; SITE DIRECTED MUTAGENESIS; WILD TYPE;

BACILLUS; DNA, BACTERIAL; ESCHERICHIA COLI; GLUCOSYLTRANSFERASES; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; MUTANT PROTEINS; PROTEIN SORTING SIGNALS; RECOMBINANT PROTEINS; SEQUENCE ANALYSIS, DNA;

BACILLUS SP.; ESCHERICHIA COLI;

EID: 84858971609     PISSN: 14641801     EISSN: 16602412     Source Type: Journal    
DOI: 10.1159/000336524     Document Type: Article

References (36)

1. Akita M, Sasaki S, Matsuyama S, Mizushima S: Seca interacts with secretory proteins by recognizing the positive charge at the amino terminus of the signal peptide in Escherichia coli . J Biol Chem 1990; 265: 8164-8169.
2. Baneyx F: Recombinant protein expression in Escherichia coli. Curr Opin Biotechnol 1999; 10: 411-421. (Pubitemid 29457264)
3. Baneyx F, Mujacic M: Recombinant protein folding and misfolding in Escherichia coli . Nat Biotechnol 2004; 22: 1399-1408.
4. Bensing BA, Siboo IR, Sullam PM: Glycine residues in the hydrophobic core of the GSPB signal sequence route export toward the accessory sec pathway. J Bacteriol 2007; 189: 3846-3854. (Pubitemid 46740182)
5. Fang N, Zhong C-Q, Liang X, Tang X-F, Tang B: Improvement of extracellular production of a thermophilic subtilase expressed in Escherichia coli by random mutagenesis of its Nterminal propeptide. Appl Microbiol Biotechnol 2010; 85: 1473-1481.
6. Fu X-Y: Extracellular accumulation of recombinant protein by Escherichia coli in a defined medium. Appl Microbiol Biotechnol 2010; 88: 75-86.
7. Fu ZB, Ng KL, Lam TL, Wong WKR: Cell death caused by hyper-expression of a secretory exoglucanase in Escherichia coli . Protein Expr Purif 2005; 42: 67-77. (Pubitemid 40793395)
8. Hasenwinkle D, Jervis E, Kops O, Liu C, Lesnicki G, Haynes CA, Kilburn DG: Very high-level production and export in Escherichia coli of a cellulose binding domain for use in a generic secretion-affinity fusion system. Biotechnol Bioeng 1997; 55: 854-863. (Pubitemid 27360506)
9. Ibrahim HM, Yusoff WMW, Hamid AA, Illias RM, Omer O: Optimization of the process conditions for cyclodextrin glucanotransferase production using response surface methodology. Asian J Microbiol Biotechnol Environ Sci 2003; 5: 297-300. (Pubitemid 38035314)
10. Izard JW, Rusch SL, Kendall DA: The amino-terminal charge and core region hydrophobicity interdependently contribute to the function of signal sequences. J Biol Chem 1996; 271: 21579-21582. (Pubitemid 26293027)
11. Jain RG, Rusch SL, Kendall DA: Signal peptide cleavage regions. Functional limits on length and topological implications. J Biol Chem 1994; 269: 16305-16310.
12. Jemli S, Messaoud EB, Mabrouk SB, Bejar S: The cyclodextrin glycosyltransferase of Paenibacillus pabuli US132 strain: molecular characterization and overproduction of the recombinant enzyme. J Biomed Biotechnol 2008; 2008: 692573.
13. Jeong KJ, Lee SY: Secretory production of human leptin in Escherichia coli . Biotechnol Bioeng 2000; 67: 398-407.
14. Khokhlova OV, Nesmeyanova MA: Interaction of SecB and SecA with the N-terminal region of mature alkaline phosphatase on its secretion in Escherichia coli. Mol Biol 2003; 37: 712-718.
15. Kweon D-H, Kim S-G, Seo J-H: Purification and refolding of cyclodextrin glycosyltransferase expressed Escherichia coli . J Incl Phenom Macrocycl Chem 2004; 50: 37-41. (Pubitemid 40137315)
16. Laemmli UK: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970; 227: 680-685.
17. Lemberg MK, Bland FA, Weihofen A, Braud VM, Martoglio B: Intramembrane proteolysis of signal peptides: an essential step in the generation of HLA-E epitopes. J Immunol 2001; 167: 6441-6446. (Pubitemid 33081577)
18. Lemberg MK, Martoglio B: Requirements for signal peptide peptidase-catalyzed intramembrane proteolysis. Mol Cell 2002; 10: 735-744. (Pubitemid 35335630)
19. Li Z, Gu Z, Wang M, Du G, Wu J, Chen J: Delayed supplementation of glycine enhances extracellular secretion of the recombinant D-cyclodextrin glycosyltransferase in Escherichia coli . Appl Microbiol Biotechnol 2010; 85: 553-561.
20. Matsumi R, Atomi H, Imanaka T: Biochemical properties of a putative signal peptide peptidase from the hyperthermophilic arcaheon Thermococcus kodakaraensis KOD1. J Biotechnol 2005; 187: 7072-7080. (Pubitemid 41428884)
21. Morioka-Fujimoto K, Marumotot R, Fukuda T: Modified enterotoxin signal sequences increase secretion level of the recombinant human epidermal growth factor in Escherichia coli . J Biol Chem 1991; 266: 1728-1732. (Pubitemid 21908363)
22. Nakamura K, Furusato T, Shiroza T, Yamane K: Stable hyperproduction of Escherichia coliβ-lactamase by Bacillus subtilis grown on a 0.5 M succinate-medium using a b. Subtilisα-amylase secretion vector. Biochem Biophys Res Commun 1985; 128: 601-606. (Pubitemid 15004491)
23. Nesmeyanova MA, Karamyshev AL, Karamyshev ZN, Kalinin AE, Ksenzenko VN, Kajava AV: Positively charge lysine at the N-terminus of the signal peptide of the Escherichia coli alkaline phosphatase provides the secretion efficiency and is involved in the interaction with anionic phospholipids. FEBS Lett 1997; 403: 203-207. (Pubitemid 27081495)
24. Ni Y, Chen R: Extracellular recombinant protein production from Escherichia coli. Biotechnol Lett 2009; 31: 1705-1714.
25. Ong RM, Goh KM, Mahadi NM, Hassan O, Rahman RNZRA, Illias RM: Cloning, extracellular expression and characterization of a predominantβ-cgtase from Bacillus sp. G1 in E. coli . J Ind Microbiol Biotechnol 2008; 35: 1705-1714.
26. Pugsley A, Francetic O: Protein secretion in Escherichia coli K-12: dead or alive? Cell Mol Life Sci 1998; 54: 347-352. (Pubitemid 28192741)
27. Pugsley AP: The complete general secretory pathway in Gram-negative bacteria. Microbiol Mol Biol Rev 1993; 57: 50-108. (Pubitemid 23078438)
28. Rusch S, Mascolo C, Kebir M, Kendall D: Juxtaposition of signal-peptide charge and core region hydrophobicity is critical for functional signal peptides. Arch Microbiol 2002; 178: 306-310. (Pubitemid 35025073)
29. Sanchez L, Ayala M, Freyre F, Pedroso I, Bell H, Falcon V, Gavilondo JV: High cytoplasmic expression in E. coli, purification, and in vitro refolding of a single chain Fv antibody fragment against the hepatitis B surface antigen. J Biotechnol 1999; 72: 13-20. (Pubitemid 29259867)
30. Shokri A, Sandén AM, Larsson G: Growth ratedependent changes in Escherichia coli membrane structure and protein leakage. Appl Microbiol Biotechnol 2002; 58: 386-392. (Pubitemid 34185585)
31. Shokri A, Sandén AM, Larsson G: Cell and process design for targeting of recombinant protein into the culture medium of Escherichia coli . Appl Microbiol Biotechnol 2003; 60: 654-664. (Pubitemid 36286671)
32. Takimura Y, Kato M, Ohta T, Yamagata H, Udaka S: Secretion of human interleukin-2 in biologically active form by Bacillus brevis directly into culture medium. Biosci Biotech Biochem 1997; 61: 1858-1861. (Pubitemid 127471779)
33. Von Heijne G, Abrahmsen L: Species-specific variation in signal peptide design. Implications for protein secretion in foreign hosts. FEBS Lett 1989; 244: 439-446. (Pubitemid 19067168)
34. Yamabhai M, Emrat S, Sukasem S, Pesatcha P, Jaruseranee N, Buranabanyat B: Secretion of recombinant Bacillus hydrolytic enzymes using Escherichia coli expression systems. J Biotechnol 2008; 133: 50-57. (Pubitemid 350101872)
35. Yoon SH, Kim SK, Kim JF: Secretory production of recombinant proteins in Escherichia coli . Biotechnology 2010; 4: 23-29.
36. Zwizinski C, Wickner W: Purification and characterization of leader (signal) peptidase from Escherichia coli . J Biol Chem 1980; 255: 7973-7977. (Pubitemid 11231929)