A simplified counter-selection recombineering protocol for creating fluorescent protein reporter constructs directly from C. elegans fosmid genomic clones

BMC Biotechnology

Volumn 13, Issue , 2013, Pages

  Hirani, Nisha ^a   Westenberg, Marcel ^a,d   Gami, Minaxi S ^a   Davis, Paul ^b   Hope, Ian A ^c   Dolphin, Colin T ^a  

^a KING'S COLLEGE LONDON   (United Kingdom)

^b WELLCOME TRUST SANGER INSTITUTE   (United Kingdom)

^c UNIVERSITY OF LEEDS   (United Kingdom)

^d WAGENINGEN UNIVERSITY   (Netherlands)

Author keywords

C. elegans; Deoxyribose phosphate aldolase; Fluorescent protein; Fosmid; Metallocarboxypeptidase; Peroxiredoxin; Recombineering

Indexed keywords

ALDOLASES; ELEGANS; FLUORESCENT PROTEIN; FOSMID; METALLOCARBOXYPEPTIDASE; PEROXIREDOXIN; RECOMBINEERING;

FLUORESCENCE; GENE EXPRESSION; PROTEINS;

CLONING;

CYAN FLUORESCENT PROTEIN;

ARTICLE; CAENORHABDITIS ELEGANS; CONTROLLED STUDY; FUSION GENE; GENE CASSETTE; GENE CONSTRUCT; GENE EXPRESSION; GENE SEQUENCE; GENETIC ENGINEERING; MOLECULAR CLONING; NONHUMAN; POLYMERASE CHAIN REACTION; RECOMBINEERING; REPORTER GENE; RESTRICTION FRAGMENT;

ANIMALS; CAENORHABDITIS ELEGANS; GENE EXPRESSION; GENES, REPORTER; GENETIC ENGINEERING; GENETIC VECTORS; GENOME; LUMINESCENT PROTEINS;

CAENORHABDITIS ELEGANS;

EID: 84871713284     PISSN: None     EISSN: 14726750     Source Type: Journal    
DOI: 10.1186/1472-6750-13-1     Document Type: Article

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