Structure-guided evolution of cyan fluorescent proteins towards a quantum yield of 93%

Nature Communications

Volumn 3, Issue , 2012, Pages

  Goedhart, Joachim ^a   Von Stetten, David ^b   Noirclerc Savoye, Marjolaine ^c,d,e   Lelimousin, Mickaël ^f   Joosen, Linda ^a   Hink, Mark A ^a   Van Weeren, Laura ^a   Gadella, Theodorus W J ^a   Royant, Antoine ^b,c,d,e  

^a UNIVERSITY OF AMSTERDAM   (Netherlands)

^b EUROPEAN SYNCHROTRON RADIATION FACILITY   (France)

^c INSTITUT DE BIOLOGIE STRUCTURALE   (France)

^d CEA GRENOBLE   (France)

^e UNIV GRENOBLE ALPES   (France)

^f UNIVERSITY OF OXFORD   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

CYAN FLUORESCENT PROTEIN; ENHANCED CYAN FLUORESCENT PROTEIN; MTURQUOISE PROTEIN; SUPER CYAN FLUORESCENT PROTEIN 3A; UNCLASSIFIED DRUG; BACTERIAL PROTEIN; GREEN FLUORESCENT PROTEIN;

ARTICLE; BETA CHAIN; CHROMATOPHORE; CRYSTALLIZATION; FLUORESCENCE RESONANCE ENERGY TRANSFER; HYDROGEN BOND; IN VITRO STUDY; IN VIVO STUDY; MOLECULAR EVOLUTION; MUTAGENESIS; PROTEIN ANALYSIS; PROTEIN CONFORMATION; PROTEIN EXPRESSION; PROTEIN PURIFICATION; PROTEIN STABILITY; QUANTUM YIELD; X RAY CRYSTALLOGRAPHY; CHEMISTRY; GENETICS; HELA CELL; HUMAN; METHODOLOGY; PROTEIN SECONDARY STRUCTURE; SITE DIRECTED MUTAGENESIS; TUMOR CELL LINE; ULTRASTRUCTURE;

BACTERIAL PROTEINS; CELL LINE, TUMOR; CRYSTALLOGRAPHY, X-RAY; GREEN FLUORESCENT PROTEINS; HELA CELLS; HUMANS; HYDROGEN BONDING; MUTAGENESIS, SITE-DIRECTED; PROTEIN CONFORMATION; PROTEIN STRUCTURE, SECONDARY;

EID: 84859173050     PISSN: None     EISSN: 20411723     Source Type: Journal    
DOI: 10.1038/ncomms1738     Document Type: Article

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