A simplified, robust, and streamlined procedure for the production of C. elegans transgenes via recombineering

BMC Developmental Biology

Volumn 8, Issue , 2008, Pages

  Zhang, Yue ^a,b   Nash, Lindsey ^a   Fisher, Alfred L ^a  

^a UNIVERSITY OF PITTSBURGH SCHOOL OF MEDICINE   (United States)

^b BETH ISRAEL DEACONESS MEDICAL CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CAENORHABDITIS ELEGANS PROTEIN; HYBRID PROTEIN;

ANIMAL; ARTICLE; CAENORHABDITIS ELEGANS; EVALUATION; GENETIC ENGINEERING; GENETICS; METABOLISM; METHODOLOGY; PLASMID; RNA INTERFERENCE; TRANSGENE; TRANSGENIC ANIMAL;

ANIMALS; ANIMALS, GENETICALLY MODIFIED; CAENORHABDITIS ELEGANS; CAENORHABDITIS ELEGANS PROTEINS; GENETIC ENGINEERING; PLASMIDS; RECOMBINANT FUSION PROTEINS; RNA INTERFERENCE; TRANSGENES;

EID: 58649115776     PISSN: None     EISSN: 1471213X     Source Type: Journal    
DOI: 10.1186/1471-213X-8-119     Document Type: Article

References (26)

1. DNA transformation. C Mello A Fire, Methods Cell Biol 1995 48 451 482 8531738
2. A modular set of lacZ fusion vectors for studying gene expression in Caenorhabditis elegans. A Fire SW Harrison D Dixon, Gene 1990 93 189 198 2121610
3. Green fluorescent protein as a marker for gene expression. M Chalfie Y Tu G Euskirchen WW Ward DC Prasher, Science 1994 263 802 805 8303295
4. A first version of the Caenorhabditis elegans Promoterome. D Dupuy QR Li B Deplancke M Boxem T Hao P Lamesch R Sequerra S Bosak L Doucette-Stamm IA Hope DE Hill AJ Walhout M Vidal, Genome Res 2004 14 2169 2175 15489340
5. Large-scale gene expression pattern analysis, in situ, in Caenorhabditis elegans. S Bamps IA Hope, Brief Funct Genomic Proteomic 2008 18332038
6. Caenorhabditis elegans reporter fusion genes generated by seamless modification of large genomic DNA clones. CT Dolphin IA Hope, Nucleic Acids Res 2006 34 e72 16717278
7. A recombineering pipeline for functional genomics applied to Caenorhabditis elegans. M Sarov S Schneider A Pozniakovski A Roguev S Ernst Y Zhang AA Hyman AF Stewart, Nat Methods 2006 3 839 844 16990816
8. Creation of low-copy integrated transgenic lines in Caenorhabditis elegans. V Praitis E Casey D Collar J Austin, Genetics 2001 157 1217 1226 11238406
9. Simple and highly efficient BAC recombineering using galK selection. S Warming N Costantino DL Court NA Jenkins NG Copeland, Nucleic Acids Res 2005 33 e36 15731329
10. An improved suicide vector for construction of chromosomal insertion mutations in bacteria. RJ Penfold JM Pemberton, Gene 1992 118 145 146 1511879
11. Construction of human artificial chromosome vectors by recombineering. G Kotzamanis W Cheung H Abdulrazzak S Perez-Luz S Howe H Cooke C Huxley, Gene 2005 351 29 38 15837432
12. Synergy between tetA and rpsL provides high-stringency positive and negative selection in bacterial artificial chromosome vectors. TA Stavropoulos CA Strathdee, Genomics 2001 72 99 104 11247671
13. A rapid PCR fidelity assay. JM Flaman T Frebourg V Moreau F Charbonnier C Martin C Ishioka SH Friend R Iggo, Nucleic Acids Res 1994 22 3259 3260 8065949
14. The Caenorhabditis elegans K10C2.4 Gene Encodes a Member of the Fumarylacetoacetate Hydrolase Family: A CAENORHABDITIS ELEGANS MODEL OF TYPE I TYROSINEMIA. AL Fisher KE Page GJ Lithgow L Nash, J Biol Chem 2008 283 9127 9135 18227072
15. A conserved protein network controls assembly of the outer kinetochore and its ability to sustain tension. IM Cheeseman S Niessen S Anderson F Hyndman JR Yates III K Oegema A Desai, Genes Dev 2004 18 2255 2268 15371340
16. Functional proteomics reveals the biochemical niche of C. elegans DCR-1 in multiple small-RNA-mediated pathways. TF Duchaine JA Wohlschlegel S Kennedy Y Bei D Conte Jr K Pang DR Brownell S Harding S Mitani G Ruvkun JR Yates III CC Mello, Cell 2006 124 343 354 16439208
17. A conserved pathway to activate BRCA1-dependent ubiquitylation at DNA damage sites. J Polanowska JS Martin T Garcia-Muse MI Petalcorin SJ Boulton, EMBO J 2006 25 2178 2188 16628214
18. CIF-1, a shared subunit of the COP9/signalosome and eukaryotic initiation factor 3 complexes, regulates MEL-26 levels in the Caenorhabditis elegans embryo. S Luke-Glaser M Roy B Larsen BT Le P Metalnikov M Tyers M Peter L Pintard, Mol Cell Biol 2007 27 4526 4540 17403899
19. Proteomics in Caenorhabditis elegans. A Audhya A Desai, Brief Funct Genomic Proteomic 2008 18372286
20. A generic protein purification method for protein complex characterization and proteome exploration. G Rigaut A Shevchenko B Rutz M Wilm M Mann B Seraphin, Nat Biotechnol 1999 17 1030 1032 10504710
21. Identification and characterization of novel nicotinic receptor-associated proteins in Caenorhabditis elegans. A Gottschalk RB Almedom T Schedletzky SD Anderson JR Yates III WR Schafer, EMBO J 2005 24 2566 2578 15990870
22. A modified tandem affinity purification strategy identifies cofactors of the Drosophila nuclear receptor dHNF4. P Yang HM Sampson HM Krause, Proteomics 2006 6 927 935 16400689
23. The tandem affinity purification (TAP) method: a general procedure of protein complex purification. O Puig F Caspary G Rigaut B Rutz E Bouveret E Bragado-Nilsson M Wilm B Seraphin, Methods 2001 24 218 229 11403571
24. Identification and cloning of unc-119, a gene expressed in the Caenorhabditis elegans nervous system. M Maduro D Pilgrim, Genetics 1995 141 977 988 8582641
25. Homologous gene targeting in Caenorhabditis elegans by biolistic transformation. E Berezikov CI Bargmann RH Plasterk, Nucleic Acids Res 2004 32 e40 14982959
26. Ingestion of bacterially expressed dsRNAs can produce specific and potent genetic interference in Caenorhabditis elegans. L Timmons DL Court A Fire, Gene 2001 263 103 112 11223248