Comparison of sequencing (Barcode Region) and sequence-tagged-site PCR for Blastocystis subtyping

Journal of Clinical Microbiology

Volumn 51, Issue 1, 2013, Pages 190-194

  Stensvold, Christen Rune ^a  

^a STATENS SERUM INSTITUT   (Denmark)

Author keywords

[No Author keywords available]

Indexed keywords

RIBOSOME DNA; SMALL SUBUNIT RIBOSOMAL DNA; UNCLASSIFIED DRUG;

ALLELE; ARTICLE; BLASTOCYSTIS; BLASTOCYSTOSIS; CONTROLLED STUDY; DNA BARCODING; DNA EXTRACTION; DNA SEQUENCE; FALSE NEGATIVE RESULT; FECES ANALYSIS; GENE LOCUS; GENETIC VARIABILITY; INTERMETHOD COMPARISON; MOLECULAR TYPING; NONHUMAN; NUCLEOTIDE SEQUENCE; PARASITE CULTIVATION; PARASITE EXAMINATION; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SEQUENCE TAGGED SITE;

BLASTOCYSTIS; BLASTOCYSTIS INFECTIONS; DNA BARCODING, TAXONOMIC; DNA PRIMERS; DNA, PROTOZOAN; GENOTYPE; HUMANS; MOLECULAR EPIDEMIOLOGY; PARASITOLOGY; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SEQUENCE ANALYSIS, DNA; SEQUENCE TAGGED SITES;

BLASTOCYSTIS; EUKARYOTA;

EID: 84871689642     PISSN: 00951137     EISSN: 1098660X     Source Type: Journal    
DOI: 10.1128/JCM.02541-12     Document Type: Article

References (24)

1. Böhm-Gloning B, Knobloch J, Walderich B. 1997. Five subgroups of Blastocystis hominis from symptomatic and asymptomatic patients revealed by restriction site analysis of PCR-amplified 16S-like rDNA. Trop. Med. Int. Health 2:771-778. (Pubitemid 27347993)
2. Clark CG. 1997. Extensive genetic diversity in Blastocystis hominis. Mol. Biochem. Parasitol. 87:79-83. (Pubitemid 27293336)
3. Stensvold CR, Suresh GK, Tan KS, Thompson RC, Traub RJ, Viscogliosi E, Yoshikawa H, Clark CG. 2007. Terminology for Blastocystis subtypes: a consensus. Trends Parasitol. 23:93-96.
4. Clark CG, Alfellani M, van der Giezen M, Stensvold CR. Recent developments in Blastocystis research. Adv. Parasitol., in press.
5. Stensvold CR. 2012. Thinking Blastocystis out of the box. Trends Parasitol. 28:305.
6. Fayer R, Santin M, Macarisin D. 2012. Detection of concurrent infection of dairy cattle with Blastocystis, Cryptosporidium, Giardia, and Enterocytozoon by molecular and microscopic methods. Parasitol. Res. 111:1349-1355.
7. Parkar U, Traub RJ, Vitali S, Elliot A, Levecke B, Robertson I, Geurden T, Steele J, Drake B, Thompson RC. 2010. Molecular characterization of Blastocystis isolates from zoo animals and their animal-keepers. Vet. Parasitol. 169:8-17.
8. Stensvold CR, Nielsen HV, Mølbak K, Smith HV. 2009. Pursuing the clinical significance of Blastocystis: diagnostic limitations. Trends Parasitol. 25:23-29.
9. Domínguez-Márquez MV, Guna R, Muñoz C, Gómez-Muñoz MT, Borrás R. 2009. High prevalence of subtype 4 among isolates of Blastocystis hominis from symptomatic patients of a health district of Valencia (Spain). Parasitol. Res. 105:949-955.
10. Stensvold CR, Christiansen DB, Olsen KE, Nielsen HV. 2011. Blastocystis sp. subtype 4 is common in Danish Blastocystis-positive patients presenting with acute diarrhea. Am. J. Trop. Med. Hyg. 84:883-885.
11. Yoshikawa H, Wu Z, Kimata I, Iseki M, Ali IK, Hossain MB, Zaman V, Haque R, Takahashi Y. 2004. Polymerase chain reaction-based genotype classification among human Blastocystis hominis populations isolated from different countries. Parasitol. Res. 92:22-29. (Pubitemid 38167933)
12. Scicluna SM, Tawari B, Clark CG. 2006. DNA barcoding of Blastocystis. Protist 157:77-85. (Pubitemid 43220718)
13. Forsell J, Granlund M, Stensvold CR, Clark GC, Evengård B. 2012. Subtype analysis of Blastocystis isolates in Swedish patients. Eur. J. Clin. Microbiol. Infect. Dis. 31:1689-1696.
14. Malheiros AF, Stensvold CR, Clark CG, Braga GB, Shaw JJ. 2011. Short report: molecular characterization of Blastocystis obtained from members of the Indigenous Tapirape ethnic group from the Brazilian Amazon region, Brazil. Am. J. Trop. Med. Hyg. 85:1050-1053.
15. Souppart L, Moussa H, Cian A, Sanciu G, Poirier P, El Alaoui H, Delbac F, Boorom K, Delhaes L, Dei-Cas E, Viscogliosi E. 2010. Subtype analysis of Blastocystis isolates from symptomatic patients in Egypt. Parasitol. Res. 106:505-511.
16. Stensvold CR, Ahmed UN, Andersen LO, Nielsen HV. 2012. Development and evaluation of a genus-specific, probe-based, internal process controlled real-time PCR assay for sensitive and specific detection of Blastocystis. J. Clin. Microbiol. 50:1847-1851.
17. Stensvold CR, Alfellani M, Clark CG. 2012. Levels of genetic diversity vary dramatically between Blastocystis subtypes. Infect. Genet. Evol. 12:263-273.
18. Stensvold CR, Alfellani MA, Nørskov-Lauritsen S, Prip K, Victory EL, Maddox C, Nielsen HV, Clark CG. 2009. Subtype distribution of Blastocystis isolates from synanthropic and zoo animals and identification of a new subtype. Int. J. Parasitol. 39:473-479.
19. Stensvold CR, Jespersgaard C, Dinesen K, Sorensen JF, Molbak K, Nielsen HV. 2007. Prevalence of Dientamoeba fragilis genotypes in different Danish populations as assessed by PCR and SNP genotyping. Trop. Med. Int. Health 12(Suppl 1):182.
20. Stensvold CR, Nielsen HV. 2011. Comparison of microscopy and PCR for the detection of intestinal parasites in Danish patients supports incentive for molecular screening platforms. J. Clin. Microbiol. 50:540-541.
21. Hussein EM, Hussein AM, Eida MM, Atwa MM. 2008. Pathophysiological variability of different genotypes of human Blastocystis hominis Egyptian isolates in experimentally infected rats. Parasitol. Res. 102:853-860.
22. Fouad SA, Basyoni MM, Fahmy RA, Kobaisi MH. 2011. The pathogenic role of different Blastocystis hominis genotypes isolated from patients with irritable bowel syndrome. Arab. J. Gastroenterol. 12:194-200.
23. Moosavi A, Haghighi A, Mojarad EN, Zayeri F, Alebouyeh M, Khazan H, Kazemi B, Zali MR. Genetic variability of Blastocystis sp. isolated from symptomatic and asymptomatic individuals in Iran. Parasitol. Res., in press.
24. Poirier P, Wawrzyniak I, Albert A, El Alaoui H, Delbac F, Livrelli V. 2011. Development and evaluation of a real-time PCR assay for detection and quantification of Blastocystis parasites in human stool samples: prospective study of patients with hematological malignancies. J. Clin. Microbiol. 49:975-983.