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Volumn 50, Issue 6, 2012, Pages 1847-1851

Development and evaluation of a genus-specific, probe-based, internal-process-controlled real-time PCR assay for sensitive and specific detection of Blastocystis spp

Author keywords

[No Author keywords available]

Indexed keywords

GENOMIC DNA; NUCLEASE; PROTOZOAL DNA;

EID: 84862077617     PISSN: 00951137     EISSN: 1098660X     Source Type: Journal    
DOI: 10.1128/JCM.00007-12     Document Type: Article
Times cited : (84)

References (42)
  • 1
    • 0034467480 scopus 로고    scopus 로고
    • Comparison of antigen-capture ELISA to stool-culture methods for the detection of asymptomatic Entamoeba species infection in Kafer Daoud, Egypt
    • Abd-Alla MD, Wahib AA, Ravdin JI. 2000. Comparison of antigencapture ELISA to stool-culture methods for the detection of asymptomatic Entamoeba species infection in Kafer Daoud, Egypt. Am. J. Trop. Med. Hyg. 62:579-582. (Pubitemid 32210478)
    • (2000) American Journal of Tropical Medicine and Hygiene , vol.62 , Issue.5 , pp. 579-582
    • Abd-Alla, M.D.1    Wahib, A.A.2    Ravdin, J.I.3
  • 2
    • 0024297354 scopus 로고
    • Multiple sequence alignment with hierarchical clustering
    • Corpet F. 1988. Multiple sequence alignment with hierarchical clustering. Nucleic Acids Res. 16:10881-10890.
    • (1988) Nucleic Acids Res. , vol.16 , pp. 10881-10890
    • Corpet, F.1
  • 3
    • 46749120545 scopus 로고    scopus 로고
    • A possible link between subtype 2 and asymptomatic infections of Blastocystis hominis
    • Dogruman-Al F, Dagci H, Yoshikawa H, Kurt O, Demirel M. 2008. A possible link between subtype 2 and asymptomatic infections of Blastocystis hominis. Parasitol. Res. 103:685-689.
    • (2008) Parasitol. Res. , vol.103 , pp. 685-689
    • Dogruman-Al, F.1    Dagci, H.2    Yoshikawa, H.3    Kurt, O.4    Demirel, M.5
  • 4
    • 70349661872 scopus 로고    scopus 로고
    • Blastocystis subtypes in irritable bowel syndrome and inflammatory bowel disease in Ankara, Turkey
    • Dogruman-Al F, et al. 2009. Blastocystis subtypes in irritable bowel syndrome and inflammatory bowel disease in Ankara, Turkey. Mem. Inst. Oswaldo Cruz 104:724-727.
    • (2009) Mem. Inst. Oswaldo Cruz , vol.104 , pp. 724-727
    • Dogruman-Al, F.1
  • 5
    • 71449109295 scopus 로고    scopus 로고
    • PCR-based subtyping of Blastocystis isolates from symptomatic and asymptomatic individuals in a major hospital in Ankara, Turkey
    • Dogruman-Al F, Yoshikawa H, Kustimur S, Balaban N. 2009. PCR-based subtyping of Blastocystis isolates from symptomatic and asymptomatic individuals in a major hospital in Ankara, Turkey. Parasitol. Res. 106:263-268.
    • (2009) Parasitol. Res. , vol.106 , pp. 263-268
    • Dogruman-Al, F.1    Yoshikawa, H.2    Kustimur, S.3    Balaban, N.4
  • 6
    • 69249203717 scopus 로고    scopus 로고
    • High prevalence of subtype 4 among isolates of Blastocystis hominis from symptomatic patients of a health district of Valencia (Spain)
    • Domínguez-Márquez MV, Guna R, Muñoz C, Gómez-Muñoz MT, Borrás R. 2009. High prevalence of subtype 4 among isolates of Blastocystis hominis from symptomatic patients of a health district of Valencia (Spain). Parasitol. Res. 105:949-955.
    • (2009) Parasitol. Res. , vol.105 , pp. 949-955
    • Domínguez-Márquez, M.V.1    Guna, R.2    Muñoz, C.3    Gómez-Muñoz, M.T.4    Borrás, R.5
  • 7
    • 10744232249 scopus 로고    scopus 로고
    • Use of TaqMan 5′ Nuclease Real-Time PCR for Quantitative Detection of Mycoplasma genitalium DNA in Males with and without Urethritis Who Were Attendees at a Sexually Transmitted Disease Clinic
    • DOI 10.1128/JCM.42.2.683-692.2004
    • Jensen JS, Björnelius E, Dohn B, Lidbrink P. 2004. Use of TaqMan 5′ nuclease real-time PCR for quantitative detection of Mycoplasma genitalium DNA in males with and without urethritis who were attendees at a sexually transmitted disease clinic. J. Clin. Microbiol. 42:683-692. (Pubitemid 38228388)
    • (2004) Journal of Clinical Microbiology , vol.42 , Issue.2 , pp. 683-692
    • Jensen, J.S.1    Bjornelius, E.2    Dohn, B.3    Lidbrink, P.4
  • 8
    • 12244299910 scopus 로고    scopus 로고
    • Detection of Mycoplasma genitalium by PCR amplification of the 16S rRNA gene
    • DOI 10.1128/JCM.41.1.261-266.2003
    • Jensen JS, Borre MB, Dohn B. 2003. Detection of Mycoplasma genitalium by PCR amplification of the 16S rRNA gene. J. Clin. Microbiol. 41:261-266. (Pubitemid 36104199)
    • (2003) Journal of Clinical Microbiology , vol.41 , Issue.1 , pp. 261-266
    • Jensen, J.S.1    Borre, M.B.2    Dohn, B.3
  • 9
    • 33747068779 scopus 로고    scopus 로고
    • Candida species in stool, symptoms and complaints in general practice - A cross-sectional study of 308 outpatients
    • DOI 10.1111/j.1439-0507.2006.01244.x
    • Jobst D, Kraft K. 2006. Candida species in stool, symptoms and complaints in general practice - a cross-sectional study of 308 outpatients. Mycoses 49:415-420. (Pubitemid 44213885)
    • (2006) Mycoses , vol.49 , Issue.5 , pp. 415-420
    • Jobst, D.1    Kraft, K.2
  • 10
    • 78650827616 scopus 로고    scopus 로고
    • BIGSdb: Scalable analysis of bacterial genome variation at the population level
    • Jolley KA, Maiden MC. 2010. BIGSdb: scalable analysis of bacterial genome variation at the population level. BMC Bioinformatics 11:595.
    • (2010) BMC Bioinformatics , vol.11 , pp. 595
    • Jolley, K.A.1    Maiden, M.C.2
  • 11
    • 46749101638 scopus 로고    scopus 로고
    • Detection of Blastocystis from stool samples using real-time PCR
    • Jones MS, et al. 2008. Detection of Blastocystis from stool samples using real-time PCR. Parasitol. Res. 103:551-557.
    • (2008) Parasitol. Res. , vol.103 , pp. 551-557
    • Jones, M.S.1
  • 12
    • 58649098329 scopus 로고    scopus 로고
    • Association of Blastocystis subtype 3 and 1 with patients from an Oregon community presenting with chronic gastrointestinal illness
    • Jones MS, et al. 2009. Association of Blastocystis subtype 3 and 1 with patients from an Oregon community presenting with chronic gastrointestinal illness. Parasitol. Res. 104:341-345.
    • (2009) Parasitol. Res. , vol.104 , pp. 341-345
    • Jones, M.S.1
  • 14
    • 34548601136 scopus 로고    scopus 로고
    • Molecular epidemiology of human Blastocystis in a village in Yunnan province, China
    • Li LH, et al. 2007. Molecular epidemiology of human Blastocystis in a village in Yunnan province, China. Parasitol. Int. 56:281-286.
    • (2007) Parasitol. Int. , vol.56 , pp. 281-286
    • Li, L.H.1
  • 15
    • 77956817903 scopus 로고    scopus 로고
    • Fungi isolated from the stool in patients with gastrointestinal disorders in 2005-2009
    • Macura AB, Witalis J. 2010. Fungi isolated from the stool in patients with gastrointestinal disorders in 2005-2009. Przegl. Epidemiol. 64:313-317.
    • (2010) Przegl. Epidemiol. , vol.64 , pp. 313-317
    • Macura, A.B.1    Witalis, J.2
  • 16
    • 80052329648 scopus 로고    scopus 로고
    • Molecular subtyping of Blastocystis sp. isolates from symptomatic patients in Italy
    • Meloni D, et al. 2011. Molecular subtyping of Blastocystis sp. isolates from symptomatic patients in Italy. Parasitol. Res. 109:613-619.
    • (2011) Parasitol. Res. , vol.109 , pp. 613-619
    • Meloni, D.1
  • 17
    • 42249085065 scopus 로고    scopus 로고
    • High prevalence of Blastocystis sp. in pigs reared under intensive growing systems: Frequency of ribotypes and associated risk factors
    • Navarro C, et al. 2008. High prevalence of Blastocystis sp. in pigs reared under intensive growing systems: frequency of ribotypes and associated risk factors. Vet. Parasitol. 153:347-358.
    • (2008) Vet. Parasitol. , vol.153 , pp. 347-358
    • Navarro, C.1
  • 18
    • 19944429449 scopus 로고    scopus 로고
    • Molecular phylogenies of Blastocystis isolates from different hosts: Implications for genetic diversity, identification of species, and zoonosis
    • Noël C, et al. 2005. Molecular phylogenies of Blastocystis isolates from different hosts: implications for genetic diversity, identification of species, and zoonosis. J. Clin. Microbiol. 43:348-355.
    • (2005) J. Clin. Microbiol. , vol.43 , pp. 348-355
    • Noël, C.1
  • 19
    • 44749084790 scopus 로고    scopus 로고
    • Molecular epidemiology of Blastocystis infections in Turkey
    • Ozyurt M, et al. 2008. Molecular epidemiology of Blastocystis infections in Turkey. Parasitol. Int. 57:300-306.
    • (2008) Parasitol. Int. , vol.57 , pp. 300-306
    • Ozyurt, M.1
  • 21
    • 77951093510 scopus 로고    scopus 로고
    • Molecular characterization of Blastocystis isolates from zoo animals and their animal-keepers
    • Parkar U, et al. 2010. Molecular characterization of Blastocystis isolates from zoo animals and their animal-keepers. Vet. Parasitol. 169:8-17.
    • (2010) Vet. Parasitol. , vol.169 , pp. 8-17
    • Parkar, U.1
  • 22
    • 79952353107 scopus 로고    scopus 로고
    • Development and evaluation of a real-time PCR assay for detection and quantification of blastocystis parasites in human stool samples: Prospective study of patients with hematological malignancies
    • Poirier P, et al. 2011. Development and evaluation of a real-time PCR assay for detection and quantification of blastocystis parasites in human stool samples: prospective study of patients with hematological malignancies. J. Clin. Microbiol. 49:975-983.
    • (2011) J. Clin. Microbiol. , vol.49 , pp. 975-983
    • Poirier, P.1
  • 23
    • 65349101113 scopus 로고    scopus 로고
    • Subtype analysis of Blastocystis isolates from Blastocystis cyst excreting patients
    • Rene BA, Stensvold CR, Badsberg JH, Nielsen HV. 2009. Subtype analysis of Blastocystis isolates from Blastocystis cyst excreting patients. Am. J. Trop. Med. Hyg. 80:588-592.
    • (2009) Am. J. Trop. Med. Hyg. , vol.80 , pp. 588-592
    • Rene, B.A.1    Stensvold, C.R.2    Badsberg, J.H.3    Nielsen, H.V.4
  • 24
    • 79959377523 scopus 로고    scopus 로고
    • Development of a new PCR protocol to detect and subtype Blastocystis spp. from humans and animals
    • Santín M, Gómez-Muñoz MT, Solano-Aguilar G, Fayer R. 2011. Development of a new PCR protocol to detect and subtype Blastocystis spp. from humans and animals. Parasitol. Res. 109:205-212.
    • (2011) Parasitol. Res. , vol.109 , pp. 205-212
    • Santín, M.1    Gómez-Muñoz, M.T.2    Solano-Aguilar, G.3    Fayer, R.4
  • 25
    • 32244449463 scopus 로고    scopus 로고
    • DNA barcoding of Blastocystis
    • DOI 10.1016/j.protis.2005.12.001, PII S1434461005001100
    • Scicluna SM, Tawari B, Clark CG. 2006. DNA barcoding of blastocystis. Protist 157:77-85. (Pubitemid 43220718)
    • (2006) Protist , vol.157 , Issue.1 , pp. 77-85
    • Scicluna, S.M.1    Tawari, B.2    Clark, C.G.3
  • 26
    • 77949315358 scopus 로고    scopus 로고
    • Subtype analysis of Blastocystis isolates from symptomatic patients in Egypt
    • Souppart L, et al. 2010. Subtype analysis of Blastocystis isolates from symptomatic patients in Egypt. Parasitol. Res. 106:505-511.
    • (2010) Parasitol. Res. , vol.106 , pp. 505-511
    • Souppart, L.1
  • 27
    • 67649565979 scopus 로고    scopus 로고
    • Molecular epidemiology of human Blastocystis isolates in France
    • Souppart L, et al. 2009. Molecular epidemiology of human Blastocystis isolates in France. Parasitol. Res. 105:413-421.
    • (2009) Parasitol. Res. , vol.105 , pp. 413-421
    • Souppart, L.1
  • 28
    • 84857653596 scopus 로고    scopus 로고
    • Levels of genetic diversity vary dramatically between Blastocystis subtypes
    • Stensvold CR, Alfellani M, Clark CG. 2012. Levels of genetic diversity vary dramatically between Blastocystis subtypes. Infect. Genet. Evol. 12: 263-273.
    • (2012) Infect. Genet. Evol. , vol.12 , pp. 263-273
    • Stensvold, C.R.1    Alfellani, M.2    Clark, C.G.3
  • 29
    • 58849119916 scopus 로고    scopus 로고
    • Subtype distribution of Blastocystis isolates from synanthropic and zoo animals and identification of a new subtype
    • Stensvold CR, et al. 2009. Subtype distribution of Blastocystis isolates from synanthropic and zoo animals and identification of a new subtype. Int. J. Parasitol. 39:473-479.
    • (2009) Int. J. Parasitol. , vol.39 , pp. 473-479
    • Stensvold, C.R.1
  • 31
    • 79958707347 scopus 로고    scopus 로고
    • Blastocystis sp. subtype 4 is common in Danish Blastocystis-positive patients presenting with acute diarrhea
    • Stensvold CR, Christiansen DB, Olsen KE, Nielsen HV. 2011. Blastocystis sp. subtype 4 is common in Danish Blastocystis-positive patients presenting with acute diarrhea. Am. J. Trop. Med. Hyg. 84:883- 885.
    • (2011) Am. J. Trop. Med. Hyg. , vol.84 , pp. 883-885
    • Stensvold, C.R.1    Christiansen, D.B.2    Olsen, K.E.3    Nielsen, H.V.4
  • 32
    • 70349579088 scopus 로고    scopus 로고
    • Blastocystis: Unravelling potential risk factors and clinical significance of a common but neglected parasite
    • Stensvold CR, et al. 2009. Blastocystis: unravelling potential risk factors and clinical significance of a common but neglected parasite. Epidemiol. Infect. 137:1655-1663.
    • (2009) Epidemiol. Infect. , vol.137 , pp. 1655-1663
    • Stensvold, C.R.1
  • 33
    • 84856138256 scopus 로고    scopus 로고
    • Comparison of microscopy and PCR for the detection of intestinal parasites in Danish patients supports incentive for molecular screening platforms
    • Stensvold CR, Nielsen HV. 2012. Comparison of microscopy and PCR for the detection of intestinal parasites in Danish patients supports incentive for molecular screening platforms. J. Clin. Microbiol. 50:540-541.
    • (2012) J. Clin. Microbiol. , vol.50 , pp. 540-541
    • Stensvold, C.R.1    Nielsen, H.V.2
  • 34
    • 58149131362 scopus 로고    scopus 로고
    • Pursuing the clinical significance of Blastocystis - Diagnostic limitations
    • Stensvold CR, Nielsen HV, Mølbak K, Smith HV. 2009. Pursuing the clinical significance of Blastocystis - diagnostic limitations. Trends Parasitol. 25:23-29.
    • (2009) Trends Parasitol. , vol.25 , pp. 23-29
    • Stensvold, C.R.1    Nielsen, H.V.2    Mølbak, K.3    Smith, H.V.4
  • 35
    • 78751547149 scopus 로고    scopus 로고
    • The prevalence and clinical significance of intestinal parasites in HIV-infected patients in Denmark
    • Stensvold CR, et al. 2011. The prevalence and clinical significance of intestinal parasites in HIV-infected patients in Denmark. Scand. J. Infect. Dis. 43:129-135.
    • (2011) Scand. J. Infect. Dis. , vol.43 , pp. 129-135
    • Stensvold, C.R.1
  • 37
    • 34247108234 scopus 로고    scopus 로고
    • Blastocystis: Subtyping isolates using pyrosequencing technology
    • Stensvold CR, et al. 2007. Blastocystis: subtyping isolates using pyrosequencing technology. Exp. Parasitol. 116:111-119.
    • (2007) Exp. Parasitol. , vol.116 , pp. 111-119
    • Stensvold, C.R.1
  • 38
    • 33845274289 scopus 로고    scopus 로고
    • Detection of Blastocystis homings in unpreserved stool specimens by using polymerase chain reaction
    • DOI 10.1645/GE-840R.1
    • Stensvold R, Brillowska-Dabrowska A, Nielsen HV, Arendrup MC. 2006. Detection of Blastocystis hominis in unpreserved stool specimens by using polymerase chain reaction. J. Parasitol. 92:1081-1087. (Pubitemid 44863061)
    • (2006) Journal of Parasitology , vol.92 , Issue.5 , pp. 1081-1087
    • Stensvold, R.1    Brillowska-Dabrowska, A.2    Nielsen, H.V.3    Arendrup, M.C.4
  • 41
    • 0038682957 scopus 로고    scopus 로고
    • Molecular comparative studies among Blastocystis isolates obtained from humans and animals
    • Yoshikawa H, Wu Z, Nagano I, Takahashi Y. 2003. Molecular comparative studies among Blastocystis isolates obtained from humans and animals. J. Parasitol. 89:585-594. (Pubitemid 36828532)
    • (2003) Journal of Parasitology , vol.89 , Issue.3 , pp. 585-594
    • Yoshikawa, H.1    Wu, Z.2    Nagano, I.3    Takahashi, Y.4
  • 42
    • 60849083519 scopus 로고    scopus 로고
    • Molecular characterization of Blastocystis isolates from children and rhesus monkeys in Kathmandu, Nepal
    • Yoshikawa H, et al. 2009. Molecular characterization of Blastocystis isolates from children and rhesus monkeys in Kathmandu, Nepal. Vet. Parasitol. 160:295-300.
    • (2009) Vet. Parasitol. , vol.160 , pp. 295-300
    • Yoshikawa, H.1


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