Development and evaluation of a real-time PCR assay for detection and quantification of Blastocystis parasites in human stool samples: Prospective study of patients with hematological malignancies

Journal of Clinical Microbiology

Volumn 49, Issue 3, 2011, Pages 975-983

  Poirier, Philippe ^a,b,c   Wawrzyniak, Ivan ^a,b   Albert, Aurélie ^c   El Alaoui, Hicham ^a,b   Delbac, Frédéric ^a,b   Livrelli, Valérie ^a,c  

^a UNIVERSITÉ CLERMONT AUVERGNE   (France)

^b UNIVERSITÉ BLAISE PASCAL   (France)

^c UNIVERSITY HOSPITAL OF CLERMONT FERRAND   (France)

Author keywords

[No Author keywords available]

Indexed keywords

SMALL SUBUNIT RIBOSOMAL RNA;

ADULT; AGED; ARTICLE; BLASTOCYSTIS; CONTROLLED STUDY; DIAGNOSTIC TEST ACCURACY STUDY; FECES ANALYSIS; FECES CULTURE; FEMALE; GASTROINTESTINAL DISEASE; GASTROINTESTINAL SYMPTOM; GENE SEQUENCE; HEMATOLOGIC MALIGNANCY; HUMAN; IMMUNE STATUS; IMMUNOCOMPROMISED PATIENT; IN VITRO STUDY; MALE; MICROORGANISM DETECTION; MICROSCOPY; NONHUMAN; PARASITE LOAD; PRIORITY JOURNAL; PROSPECTIVE STUDY; REAL TIME POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY;

ADULT; AGED; BLASTOCYSTIS; BLASTOCYSTIS INFECTIONS; DNA, PROTOZOAN; DNA, RIBOSOMAL; FECES; FEMALE; HEMATOLOGIC NEOPLASMS; HUMANS; MALE; MIDDLE AGED; MOLECULAR TYPING; PARASITOLOGY; POLYMERASE CHAIN REACTION; PROSPECTIVE STUDIES; RNA, RIBOSOMAL; SENSITIVITY AND SPECIFICITY;

ANIMALIA; AVES; BLASTOCYSTIS;

EID: 79952353107     PISSN: 00951137     EISSN: 1098660X     Source Type: Journal    
DOI: 10.1128/JCM.01392-10     Document Type: Article

References (45)

1. Abe, N. 2004. Molecular and phylogenetic analysis of Blastocystis isolates from various hosts. Vet. Parasitol. 120:235-242.
2. Amin, O. M. 2002. Seasonal prevalence of intestinal parasites in the United States during 2000. Am. J. Trop. Med. Hyg. 66:799-803.
3. Cirioni, O., A. Giacometti, D. Drenaggi, F. Ancarani, and G. Scalise. 1999. Prevalence and clinical relevance of Blastocystis hominis in diverse patient cohorts. Eur. J. Epidemiol. 15:389-393.
4. Domínguez-Márquez, M. V., R. Guna, C. Munoz, M. T. Gomez-Munoz, and R. Borras. 2009. High prevalence of subtype 4 among isolates of Blastocystis hominis from symptomatic patients of a health district of Valencia (Spain). Parasitol. Res. 105:949-955.
5. Elwakil, H. S., and I. H. Hewedi. 2010. Pathogenic potential of Blastocystis hominis in laboratory mice. Parasitol. Res. 107:685-689.
6. Ghosh, K., M. Ayyaril, and V. Nirmala. 1998. Acute GVHD involving the gastrointestinal tract and infestation with Blastocystis hominis in a patient with chronic myeloid leukaemia following allogeneic bone marrow transplantation. Bone Marrow Transplant. 22:1115-1117.
7. Giacometti, A., O. Cirioni, A. Fiorentini, M. Fortuna, and G. Scalise. 1999. Irritable bowel syndrome in patients with Blastocystis hominis infection. Eur. J. Clin. Microbiol. Infect. Dis. 18:436-439.
8. Gupta, R., and K. Parsi. 2006. Chronic urticaria due to Blastocystis hominis. Australas. J. Dermatol. 47:117-119.
9. Hailemariam, G., et al. 2004. Intestinal parasitic infections in HIV/AIDS and HIV seronegative individuals in a teaching hospital, Ethiopia. Jpn. J. Infect. Dis. 57:41-43.
10. Jones, M. S., Jr., et al. 2008. Detection of Blastocystis from stool samples using real-time PCR. Parasitol. Res. 103:551-557.
11. Jones, W. R. 1946. The experimental infection of rats with Entamoeba histolytica with a method for evaluating the anti-amoebic properties of new compounds. Ann. Trop. Med. Parasitol. 40:130-140.
12. Kurniawan, A., et al. 2009. Intestinal parasitic infections in HIV/AIDS patients presenting with diarrhoea in Jakarta, Indonesia. Trans. R. Soc. Trop. Med. Hyg. 103:892-898.
13. Leder, K., M. E. Hellard, M. I. Sinclair, C. K. Fairley, and R. Wolfe. 2005. No correlation between clinical symptoms and Blastocystis hominis in immunocompetent individuals. J. Gastroenterol. Hepatol. 20:1390-1394.
14. Leelayoova, S., et al. 2002. In-vitro cultivation: a sensitive method for detecting Blastocystis hominis. Ann. Trop. Med. Parasitol. 96:803-807. (Pubitemid 36152849)
15. Levy, Y., J. George, and Y. Shoenfeld. 1996. Severe Blastocystis hominis in an elderly man. J. Infect. 33:57-59.
16. Li, L. H., et al. 2007. Cross-sectional surveys and subtype classification of human Blastocystis isolates from four epidemiological settings in China. Parasitol. Res. 102:83-90.
17. Li, L. H., et al. 2007. Molecular epidemiology of human Blastocystis in a village in Yunnan province, China. Parasitol. Int. 56:281-286.
18. Moe, K. T., et al. 1997. Experimental Blastocystis hominis infection in laboratory mice. Parasitol. Res. 83:319-325.
19. Nassir, E., et al. 2004. Blastocystis hominis as a cause of hypoalbuminemia and anasarca. Eur. J. Clin. Microbiol. Infect. Dis. 23:399-402.
20. Noël, C., et al. 2005. Molecular phylogenies of Blastocystis isolates from different hosts: implications for genetic diversity, identification of species, and zoonosis. J. Clin. Microbiol. 43:348-355.
21. Parkar, U., et al. 2010. Molecular characterization of Blastocystis isolates from zoo animals and their animal-keepers. Vet. Parasitol. 169:8-17.
22. Pegelow, K., et al. 1997. Parasitological and nutritional situation of school children in the Sukaraja district, West Java, Indonesia. Southeast Asian J. Trop. Med. Public Health 28:173-190.
23. Pinel, C., et al. 1999. Blastocystis hominis: epidemiological and clinical remarks from more than 3,500 stool examinations. Ann. Biol. Clin. (Paris) 57:601-604.
24. Rao, K., U. Sekar, K. T. Iraivan, G. Abraham, and P. Soundararajan. 2003. Blastocystis hominis - an emerging cause of diarrhoea in renal transplant recipients. J. Assoc. Physicians India 51:719-721.
25. Rene, B. A., C. R. Stensvold, J. H. Badsberg, and H. V. Nielsen. 2009. Subtype analysis of Blastocystis isolates from Blastocystis cyst excreting patients. Am. J. Trop. Med. Hyg. 80:588-592.
26. Rossignol, J. F., S. M. Kabil, M. Said, H. Samir, and A. M. Younis. 2005. Effect of nitazoxanide in persistent diarrhea and enteritis associated with Blastocystis hominis. Clin. Gastroenterol. Hepatol. 3:987-991.
27. Scicluna, S. M., B. Tawari, and C. G. Clark. 2006. DNA barcoding of Blastocystis. Protist 157:77-85.
28. Souppart, L., et al. 2009. Molecular epidemiology of human Blastocystis isolates in France. Parasitol. Res. 105:413-421.
29. Stensvold, C. R., et al. 2009. Subtype distribution of Blastocystis isolates from synanthropic and zoo animals and identification of a new subtype. Int. J. Parasitol. 39:473-479.
30. Stensvold, C. R., M. C. Arendrup, C. Jespersgaard, K. Molbak, and H. V. Nielsen. 2007. Detecting Blastocystis using parasitologic and DNA-based methods: a comparative study. Diagn. Microbiol. Infect. Dis. 59:303-307.
31. Stensvold, C. R., M. C. Arendrup, K. Molbak, and H. V. Nielsen. 2007. The prevalence of Dientamoeba fragilis in patients with suspected enteroparasitic disease in a metropolitan area in Denmark. Clin. Microbiol. Infect. 13:839-842.
32. Stensvold, C. R., H. V. Nielsen, K. Molbak, and H. V. Smith. 2009. Pursuing the clinical significance of Blastocystis - diagnostic limitations. Trends Parasitol. 25:23-29.
33. Stensvold, C. R., et al. 2007. Terminology for Blastocystis subtypes - a consensus. Trends Parasitol. 23:93-96.
34. Stenzel, D. J., and P. F. Boreham. 1996. Blastocystis hominis revisited. Clin. Microbiol. Rev. 9:563-584.
35. Suresh, K., et al. 1993. In vitro encystment and experimental infections of Blastocystis hominis. Parasitol. Res. 79:456-460.
36. Suresh, K., and H. Smith. 2004. Comparison of methods for detecting Blastocystis hominis. Eur. J. Clin. Microbiol. Infect. Dis. 23:509-511.
37. Suresh, K., H. V. Smith, and T. C. Tan. 2005. Viable Blastocystis cysts in Scottish and Malaysian sewage samples. Appl. Environ. Microbiol. 71:5619-5620.
38. Tan, K. S. 2008. New insights on classification, identification, and clinical relevance of Blastocystis spp. Clin. Microbiol. Rev. 21:639-665.
39. Taşova, Y., B. Sahin, S. Koltas, and S. Paydas. 2000. Clinical significance and frequency of Blastocystis hominis in Turkish patients with hematological malignancy. Acta Med. Okayama 54:133-136.
40. Termmathurapoj, S., et al. 2004. The usefulness of short-term in vitro cultivation for the detection and molecular study of Blastocystis hominis in stool specimens. Parasitol. Res. 93:445-447. (Pubitemid 39222519)
41. van Saanen-Ciurea, M., and H. El Achcachi. 1985. Blastocystis hominis: culture and morphological study. Experientia 41:546.
42. Yakoob, J., et al. 2004. Irritable bowel syndrome: in search of an etiology: role of Blastocystis hominis. Am. J. Trop. Med. Hyg. 70:383-385.
43. Yan, Y., et al. 2006. Genetic variability of Blastocystis hominis isolates in China. Parasitol. Res. 99:597-601.
44. Zierdt, C. H., and H. Tan. 1976. Endosymbiosis in Blastocystis hominis. Exp. Parasitol. 39:422-430.
45. Zman, V., and K. Z. Khan. 1994. A comparison of direct microscopy with culture for the diagnosis of Blastocystis hominis. Southeast Asian J. Trop. Med. Public Health 25:792-793.