Detection of viable but non-culturable Escherichia coli O157: H7 from vegetable samples using quantitative PCR with propidium monoazide and immunological assays

Food Control

Volumn 31, Issue 2, 2013, Pages 268-273

  Dinu, Laura Dorina ^a,b   Bach, Susan ^a  

^a AGRICULTURE AND AGRI FOOD CANADA   (Canada)

^b UNIVERSITY OF AGRONOMIC SCIENCES AND VETERINARY MEDICINE OF BUCHAREST   (Romania)

Author keywords

Detection; E. coli O157:H7; Immunological assays; Lettuce; Propidium monoazide quantitative PCR; Spinach; Viable but non culturable

Indexed keywords

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; LACTUCA; SPINACIA OLERACEA;

EID: 84871671293     PISSN: 09567135     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.foodcont.2012.10.020     Document Type: Article

References (25)

1. Artz R.R.E., Avery L.M., Jones D.L., Killham K. Potential pitfalls in the quantitative molecular detection of Escherichia coli O157:H7 in environmental matrices. Canadian Journal of Microbiology 2006, 52:482-488.
2. Dinu L.-D., Bach S. Induction of viable but non-culturable Escherichia coli O157:H7 in the phyllosphere of lettuce: a food safety risk factor. Applied and Environmental Microbiology 2011, 77(23):8295-8302.
3. Dinu L.-D., Delaquis P., Bach S. Nonculturable response of animal enteropathogens in the agricultural environment and implications for food safety. Journal of Food Protection 2009, 72:1342-1354.
4. Dreux N., Albagnac C., Federighi M., Carlin F., Morris C.E., Nguyen-The C. Viable but non-culturable Listeria monocytogenes on parsley leaves and absence of recovery to a culturable state. Journal of Applied Microbiology 2007, 103:1272-1281.
5. Edwards C. Problems posed by natural environments for monitoring microorganisms. Molecular Biotechnology 2000, 15:211-223.
6. Elizaquivel P., Sanchez G., Aznar R. Quantitative detection of viable foodborne E. coli O157:H7, Listeria monocytogenes and Salmonella in fresh-cut vegetables combining propidium monoazide and real-time PCR. Food Control 2012, 25:704-708.
7. Fittipaldi M., Codony F., Adrados B., Camper A.K., Morato J. Viable real-time PCR in environmental samples: can all data be interpreted directly?. Microbial Ecology 2011, 61:7-12.
8. Jacques M.-A., Morris C.E. A review of issues related to the quantification of bacteria from phyllosphere. FEMS Microbiology and Ecology 1995, 18:1-14.
9. Liang N., Dong J., Luo L., Li Y. Detection of viable Salmonella in lettuce by propidium monoazide real-time PCR. Journal of Food Science 2011, 76(4):234-237.
10. Martinon A., Cronon U.P., Wilkinson M.G. Comparison of in-house and commercial real-time PCR systems for the detection of Enterobacteriaceae and their evaluation within an interlaboratory study using intact formula samples. Food Analytical Methods 2011, 4:485-496.
11. McDougald D., Rice S.A., Weichart D., Kjelleberg S. Nonculturability: adaptation or debilitation?. FEMS Microbiology and Ecology 1998, 25:1-9.
12. Mizunoe Y., Wai S.N., Takade A., Yoshida S. Restoration of culturability of starvation-stressed and low-temperature-stressed Escherichia coli O157 by using H2O2-degrading compounds. Archives of Microbiology 1999, 172:63-67.
13. Nocker A., Camper A.K. Selective removal of DNA from dead cells of mixed bacterial communities by use of ethidium monoazide. Applied and Environmental Microbiology 2006, 72(3):1997-2004.
14. Nocker A., Camper A.K. Novel approaches toward preferential detection of viable cells using nucleic acid amplification techniques. FEMS Microbiology Letters 2009, 291:137-142.
15. Nocker A., Cheung C.Y., Camper A.K. Comparison of propidium monoazide with ethidium monoazide for differentiation of live vs. dead bacteria by selective removal of DNA from dead cells. Journal of Microbiological Methods 2006, 67:310-320.
16. Nocker A., Sossa K.E., Camper A.K. Molecular monitoring of disinfection efficacy using propidium monoazide in combination with quantitative PCR. Journal of Microbiological Methods 2007, 70:252-260.
17. Oliver J.D. The viable but non-culturable state in bacteria. Journal of Microbiology 2005, 43:93-100.
18. Oliver J.D. Recent findings on the viable but nonculturable state in pathogenic bacteria. FEMS Microbiology Reviews 2010, 34:415-425.
19. Pan Y., Breidt F. Enumeration of Listeria monocytogenes by real-time PCR with propidium monoazide and ethidium monoazide in the presence of dead cells. Applied and Environmental Microbiology 2007, 73:8028-8031.
20. Rudi K., Naterstad K., Dromtorp S.M., Holo H. Detection of viable and dead Listeria monocytogenes on gouda-like cheeses by real-time PCR. Letters in Applied Microbiology 2005, 40:301-306.
21. Sharma V.K., Dean-Nystrom E.A. Detection of enterohemorrhagic Escherichia coli O157:H7 by using a multiplex real-time PCR assay for genes encoding intimin and Shiga toxins. Veterinary Microbiology 2003, 93:247-260.
22. Shelton D.R., Karns J.S., Higgins J.A., van Kessel J.A.S., Perdue M.L., Belt K.T., et al. Impact of microbial diversity on rapid detection of enterohemorrhagic Escherichia coli in surface waters. FEMS Microbiology Letters 2006, 261:95-101.
23. Wang L., Li Y., Mustapha A. Detection of viable Escherichia coli O157:H7 by ethidium monoazide real-time PCR. Journal of Applied Microbiology 2009, 107:1719-1728.
24. Yamamoto H. Viable but nonculturable state as a general phenomenon of non-spore-forming bacteria, and its modeling. Journal of Infection and Chemotherapy 2000, 6:112-114.
25. Yukiko H.K., Miyahara M., Kumagai S. Loss of O157 antigenicity of verotoxin-producing Escherichia coli O157:H7 surviving under starvation condition. Applied and Environmental Microbiology 2000, 66(12):5540-5543.