Comparison of In-house and Commercial Real-time PCR Systems for the Detection of Enterobacteriaceae and their Evaluation Within an Interlaboratory Study Using Infant Formula Samples

Food Analytical Methods

Volumn 4, Issue 4, 2011, Pages 485-496

  Martinon, Alice ^a   Cronin, Ultan P ^a   Wilkinson, Martin G ^a  

^a UNIVERSITY OF LIMERICK   (Ireland)

Author keywords

Detection; Enterobacteriaceae; Infant formula milk; LUX primers; Real time PCR

Indexed keywords

16S RIBOSOMAL RNA; 16S RRNA; DETECTION METHODS; E. COLI; ENTEROBACTERIACEAE; FLANKING REGIONS; INFANT FORMULA; INTERLABORATORY STUDIES; REAL-TIME PCR; VIBRIONACEAE;

ESCHERICHIA COLI; GENES; POLYMERASE CHAIN REACTION; RNA;

REAL TIME SYSTEMS;

ENTEROBACTERIACEAE; ESCHERICHIA COLI; VIBRIONACEAE;

EID: 80955139408     PISSN: 19369751     EISSN: 1936976X     Source Type: Journal    
DOI: 10.1007/s12161-010-9188-7     Document Type: Article

References (46)

1. Aitichou M, Javorschi S, Ibrahim MS (2005) Two-color multiplex assay for the identification of orthopox viruses with real-time LUX-PCR. Mol Cell Probes 19(5): 323-328.
2. Anonymous (2003a) ISO 16140: 2003: microbiology of food and animal feeding stuffs-protocol for the validation of alternative methods. ISO, Geneva.
3. Anonymous (2003b) ISO 4833: 2003: microbiology of food and animal feeding stuffs-horizontal method for the enumeration of microorganisms-colony-count technique at 30 degrees C. ISO, Geneva.
4. Anonymous (2004) ISO 21528-1: 2004: microbiology of food and animal feeding stuffs-horizontal methods for the detection and enumeration of Enterobacteriaceae-Part 1: detection and enumeration by MPN technique with pre-enrichment. ISO, Geneva.
5. Anonymous (2006) ISO/TS 22964: 2006: milk and milk products-detection of Enterobacter sakazakii. ISO, Geneva.
6. Anonymous. (2010). D-LUX™ Gene Expression Assays-www. invitrogen. com/site/us/en/home/Products-and-Services/Applications/Nucleic-Acid-Amplification-and-Expression-Profiling/qRT-PCR/qPCR/D-LUX-Assays. html?CID=fl-lux. In.
7. Antal M, Farkas T, German P, Belak S, Kiss I (2007) Real-time reverse transcription-polymerase chain reaction detection of Newcastle disease virus using light upon extension fluorogenic primers. J Vet Diagn Investig 19(4): 400-404.
8. Balcazar JL, Vendrell D, de Blas I, Ruiz-Zarzuela I, Girones O, Muzquiz JL (2007) Quantitative detection of Aeromonas salmonicida in fish tissue by real-time PCR using self-quenched, fluorogenic primers. Journal of Medical Microbiology 56(3): 323-328.
9. Bayardelle P, Zafarullah M (2002) Development of oligonucleotide primers for the specific PCR-based detection of the most frequent Enterobacteriaceae species DNA using wec gene templates. Can J Microbiol 48(2): 113-122.
10. Baylis CL (2006) Enterobacteriaceae. In: CdW Blackburn (ed) Food spoilage microorganisms (Campden and Chlorleywood Food Research Association). Woodhead, UK, pp 624-667.
11. Bergthorsson U, Ochman H (1995) Heterogeneity of genome sizes among natural isolates of Escherichia coli. J Bacteriol 177(20): 5784-5789.
12. Blood RM, Curtis GDW (1995) Media for total Enterobacteriaceae, coliforms and Escherichia coli. Int J Food Microbiol 26(1): 93-115.
13. Cahill SM, Wachsmuth IK, Costarrica MD, Ben Embarek PK (2008) Powdered infant formula as a source of Salmonella infection in infants. Clin Infect Dis 46(2): 268-273.
14. Castillo M, Martin-Orue SM, Manzanilla EG, Badiola I, Martin M, Gasa J (2006) Quantification of total bacteria, enterobacteria and lactobacilli populations in pig digesta by real-time PCR. Vet Microbiol 114(1-2): 165-170.
15. Chap J, Jackson P, Siqueira R, Gaspar N, Quintas C, Park J, Osaili T, Shaker R, Jaradat Z, Hartantyo SHP, Sani NA, Estuningsih S, Forsythe SJ (2009) International survey of Cronobacter sakazakii and other Cronobacter spp. in follow up formulas and infant foods. Int J Food Microbiol 136(2): 185-188.
16. Chen R, Huang WM, Lin ZX, Zhou ZF, Yu HQ, Zhu DZ (2004) Development of a novel real-time RT-PCR assay with LUX primer for the detection of swine transmissible gastroenteritis virus. J Virol Meth 122(1): 57-61.
17. de Boer E (1998) Update on media for isolation of Enterobacteriaceae from foods. Int J Food Microbiol 45(1): 43-53.
18. Drake SL, DePaola A, Jaykus LA (2007) An overview of Vibrio vulnificus and Vibrio parahaemolyticus. Compr Rev Food Sci Food Saf 6(4): 120-144.
19. Drudy D, Mullane NR, Quinn T, Wall PG, Fanning S (2006) Enterobacter sakazakii: an emerging pathogen in powdered infant formula. Clin Infect Dis 42(7): 996-1002.
20. Edson DC, Empson S, Massey LD (2009) Pathogen detection in food microbiology laboratories: an analysis of qualitative proficiency test data, 199-2007. J Food Saf 29(4): 521-530.
21. Estuningsih S, Kress C, Hassan AA, Akineden O, Schneider E, Usleber E (2006) Enterobacteriaceae in dehydrated powdered infant formula manufactured in Indonesia and Malaysia. J Food Prot 69(12): 3013-3017.
22. Gao H, Gao QL, Zhang X, Guan C, Luo MH, Zhang HB, Liu P, Zhang HY, Li J (2010) Improved medium for detection of Klebsiella in powdered milk. J. Food Saf. 30(1): 12-23.
23. Giovannini M, Verduci E, Ghisleni D, Salvatici E, Riva E, Agostoni C (2008) Enterobacter sakazakii: an emerging problem in paediatric nutrition. J Int Med Res 36(3): 394-399.
24. Gurtler JB, Beuchat LR (2005) Performance of media for recovering stressed cells of Enterobacter sakazakii as determined using spiral plating and ecometric techniques. Appl Environ Microbiol 71(12): 7661-7669.
25. Iversen C, Forsythe SJ (2007) Comparison of media for the isolation of Enterobacter sakazakii. Appl Environ Microbiol 73(1): 48-52.
26. Joosten H, Marugg J, Stephan R, Klijn A, Jackson T, Iversen C (2008) A rapid and reliable alternative to ISO 21528-1: 2004 for detection of Enterobacteriaceae. Int J Food Microbiol 125(3): 344-346.
27. Khan IUH, Gannon V, Kent R, Koning W, Lapen DR, Miller J, Neumann N, Phillips R, Robertson W, Topp E, Bochove Ev, Edge TA (2007) Development of a rapid quantitative PCR assay for direct detection and quantification of culturable and non-culturable Escherichia coli from agriculture watersheds. J. Microbiol. Methods 69(3): 480-488.
28. Klappenbach JA, Saxman PR, Cole JR, Schmidt TM (2001) rrndb: the ribosomal RNA operon copy number database. Nucleic Acids Res 29(1): 181-184.
29. Krascsenicsova K, Trncikova T, Kaclikova E (2008) Detection and Quantification of Enterobacter sakazakii by Real-time 5'-Nuclease Polymerase Chain Reaction Targeting the palE Gene. Food Anal. Meth. 1(2): 85-94.
30. Kunchev M, Alexandrov E, Kamarinchev B, Marinov K, Gergova I, Mekouchinov K (2007) Effective LUX (Light upon eXtension) primer system for early and rapid detection of Coxiella burnetii. Biotechnology & Biotechnological Equipment 21(3): 338-340.
31. Lehmacher A, Fiegen M, Hansen B (2007) Real-time PCR of Enterobacter sakazakii in infant formula. Journal Fur Verbraucherschutz Und Lebensmittelsicherheit-Journal of Consumer Protection and Food Safety 2(2): 218-221.
32. Mackay IM, Mackay JF, Nissen MD, Sloots TP (2007) Real-time PCR: history and fluorogenic chemistries. In real-time PCR in microbiology, from diagnosis to characterization. Caister, Norfolk, pp 1-39.
33. Maheux AF, Picard FJ, Boissinot M, Bissonnette L, Paradis S, Bergeron MG (2009) Analytical comparison of nine PCR primer sets designed to detect the presence of Escherichia coli/Shigella in water samples. Water Res (Oxf) 43(12): 3019-3028.
34. McCrea JK, Liu CY, Ng LK, Wang GH (2007) Detection of the Escherichia coli pathogenic gene eae with three real-time polymerase chain reaction methods. Can J Microbiol 53(3): 398-403.
35. Mitchell SL, Budhiraja S, Thurman KA, Thacker WL, Winchell JM (2009) Evaluation of two real-time PCR chemistries for the detection of Chlamydophila pneumoniae in clinical specimens. Mol Cell Probes 23(6): 309-311.
36. Nakano S, Kobayashi T, Funabiki K, Matsumura A, Nagao Y, Yamada T (2003) Development of a PCR assay for detection of Enterobacteriaceae in foods. J Food Prot 66(10): 1798-1804.
37. Nazarenko I, Lowe B, Darfler M, Ikonomi P, Schuster D, Rashtchian A (2002) Multiplex quantitative PCR using self-quenched primers labeled with a single fluorophore. Nucleic Acids Research 30(9).
38. Nordgren J, Bucardo F, Dienus O, Svensson L, Lindgren PE (2008) Novel light-upon-extension real-time PCR assays for detection and quantification of genogroup I and II noroviruses in clinical specimens. J Clin Microbiol 46(1): 164-170.
39. Olivier A, Lee HY, Cote JC (2005) Study of the heterogeneity of 16S rRNA genes in gamma-proteobacteria: implications for phylogenetic analysis. J Gen Appl Microbiol 51(6): 395-405.
40. Qiu Y, Hu Q, Wang W (2009) Evaluation of real-time PCR in detecting enteric pathogens. China Tropical Medicine 9(1): 12-13.
41. Slavov S, Kalvatchev Z, Tsekov I, Simeonov P, Hristova L, Kotsev J, Mladenov D, Tsvetkov M (2008) Novel light-upon-extension (LUX) real-time PCR primer system for rapid detection of Polyomavirus hominis 1 (BKV) in clinical samples. Biotechnol Biotechnol Equip 22(2): 759-761.
42. Sun FL, Wu DC, Qiu ZG, Jin M, Wang XW, Li JW (2010) Development of realtime PCR systems based on SYBR Green for the specific detection and quantification of Klebsiella pneumoniae in infant formula. Food Control 21(4): 487-491.
43. Weber C, Stephan R, Druggan P, Joosten H, Iversen C (2009) Improving the enrichment procedure for Enterobacteriaceae detection. Food Microbiol 26(6): 565-572.
44. Wertz JE, Goldstone C, Gordon DM, Riley MA (2003) A molecular phylogeny of enteric bacteria and implications for a bacterial species concept. J Evol Biol 16(6): 1236-1248.
45. Wong HC, Wang PL, Chen SY, Chiu SW (2004) Resuscitation of viable but nonculturable Vibrio parahaemolyticus in a minimum salt medium. Fems Microbiology Letters 233(2): 269-275.
46. Xu R-s, Chen R, Lin C-h, Yang Z-j, Chen G-w (2008) Development of a LUX™ PCR assay for rapid detection of Vibrio parahaemolyticus. Chin Vet Sci 38(2): 1075-1079.