Immobilized metal-affinity chromatography protein-recovery screening is predictive of crystallographic structure success

Acta Crystallographica Section F: Structural Biology and Crystallization Communications

Volumn 67, Issue 9, 2011, Pages 998-1005

  Choi, Ryan ^a,b   Kelley, Angela ^a,b   Leibly, David ^a,b   Nakazawa Hewitt, Stephen ^a,b   Napuli, Alberto ^a,b   Van Voorhis, Wesley ^a,b  

^a Seattle Structural Genomics Center for Infectious Disease SSGCID   (United States)

^b UNIVERSITY OF WASHINGTON SCHOOL OF MEDICINE   (United States)

Author keywords

96 well format; auto induction; high throughput; immobilized metal affinity chromatography; protein expression; SDS PAGE; structural genomics

Indexed keywords

NICKEL; PROTEIN;

AFFINITY CHROMATOGRAPHY; ARTICLE; CHEMISTRY; ISOLATION AND PURIFICATION; METHODOLOGY; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PROTEIN BINDING; SEQUENCE ALIGNMENT; X RAY CRYSTALLOGRAPHY;

BASE SEQUENCE; CHROMATOGRAPHY, AFFINITY; CRYSTALLOGRAPHY, X-RAY; MOLECULAR SEQUENCE DATA; NICKEL; PROTEIN BINDING; PROTEINS; SEQUENCE ALIGNMENT;

ESCHERICHIA COLI;

EID: 80052632897     PISSN: None     EISSN: 17443091     Source Type: Journal    
DOI: 10.1107/S1744309111017374     Document Type: Article

References (11)

1. Alexandrov, A., Vignali, M., La Count, D. J., Quartley, E., de Vries, C., De Rosa, D., Babulski, J., Mitchell, S. F., Schoenfeld, L. W., Fields, S., Hol, W. G., Dumont, M. E., Phizicky, E. M. & Grayhack, E. J. (2004). Mol. Cell. Proteomics, 3, 934-938.
2. Alzari, P. M. et al. (2006). Acta Cryst. D62, 1103-1113.
3. Aslanidis, C. & de Jong, P. J. (1990). Nucleic Acids Res. 18, 6069-6074.
4. Benita, Y., Wise, M. J., Lok, M. C., Humphery-Smith, I. & Oosting, R. S. (2006). Mol. Cell. Proteomics, 5, 1567-1580.
5. Berrow, N. S. et al. (2006). Acta Cryst. D62, 1218-1226.
6. Bryan, C. M., Bhandari, J., Napuli, A. J., Leibly, D. J., Choi, R., Kelley, A., Van Voorhis, W. C., Edwards, T. E. & Stewart, L. J. (2011). Acta Cryst. F67,1010-1014.
7. Folkers, G. E., van Buuren, B. N. & Kaptein, R. (2004). J. Struct. Funct. Genomics, 5, 119-131.
8. Mehlin, C. et al. (2006). Mol. Biochem. Parasitol. 148, 144-160.
9. Quartley, E., Alexandrov, A., Mikucki, M., Buckner, F S., Hol, W. G., De Titta, G T., Phizicky, E. M. & Grayhack, E. J. (2009). J. Struct. Funct. Genomics, 10, 233-247.
10. Studier, F W. (2005). Protein Expr. Purif. 41, 207-234.
11. Vedadi, M. et al. (2006). Mol. Biochem. Parasitol. 151, 100-110.