Transient kinetic analysis of USP2-catalyzed deubiquitination reveals a conformational rearrangement in the K48-linked diubiquitin substrate

Biochemistry

Volumn 51, Issue 50, 2012, Pages 10075-10086

  Bozza, William P ^a   Liang, Qin ^a   Gong, Ping ^a   Zhuang, Zhihao ^a  

^a UNIVERSITY OF DELAWARE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BINDING KINETICS; CATALYTIC TURNOVER; CELLULAR PROCESS; CHAIN STRUCTURE; DATA SUPPORT; DEUBIQUITINATING ENZYMES; FLUORESCENCE ANALYSIS; GLOBAL FITTING; IN-DEPTH UNDERSTANDING; MICROSCOPIC RATE CONSTANTS; MODEL SUBSTRATES; PHYSIOLOGICAL SUBSTRATE; REGULATORY MECHANISM; STOPPED FLOW; THERAPEUTIC INTERVENTION; TRANSIENT KINETIC ANALYSIS; TRANSIENT KINETIC STUDIES; UBIQUITIN;

CATALYSIS; KINETICS; PHYSIOLOGICAL MODELS; PHYSIOLOGY; RATE CONSTANTS;

SUBSTRATES;

PROTEINASE; UBIQUITIN; UBIQUITIN SPECIFIC PROTEASE 2; UNCLASSIFIED DRUG;

ARTICLE; CONCENTRATION (PARAMETERS); ENZYME KINETICS; EQUILIBRIUM CONSTANT; FLUORESCENCE; HUMAN; HYDROLYSIS; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN BINDING; PROTEIN CONFORMATION; PROTEIN EXPRESSION; PROTEIN PURIFICATION; PROTEIN STRUCTURE; STEADY STATE; TURNOVER TIME; UBIQUITINATION;

COUMARINS; ENDOPEPTIDASES; HUMANS; KINETICS; PROTEIN CONFORMATION; UBIQUITINS;

EID: 84871258452     PISSN: 00062960     EISSN: 15204995     Source Type: Journal    
DOI: 10.1021/bi3009104     Document Type: Article

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