Automated assay of the potency of natural antioxidants using pipetting robot and spectrophotometry

Journal of Applied Biomedicine

Volumn 10, Issue 3, 2012, Pages 155-167

  Pohanka, Miroslav ^a   Sochor, Jiří ^b,c   Ruttkay Nedecký, Branislav ^b,c   Cernei, Natalia ^b,c   Adam, Vojtěch ^b,c   Hubálek, Jaromír ^b,c   Stiborová, Marie ^d   Eckschlager, Tomáš ^d   Kizek, René ^b,c  

^a UNIVERSITY OF DEFENCE   (Czech Republic)

^b MENDEL UNIVERSITY IN BRNO   (Czech Republic)

^c BRNO UNIVERSITY OF TECHNOLOGY   (Czech Republic)

^d CHARLES UNIVERSITY   (Czech Republic)

Author keywords

ABTS; antioxidant; DMPD; DPPH; FRAP; free radicals

Indexed keywords

2,2' AZINOBIS(3 ETHYLBENZOTHIAZOLINE 6 SULFONIC ACID); ANTIOXIDANT; BIPHENYL; ELLIPTICINE; FERULIC ACID; GALLIC ACID; N,N DIMETHYL 1,4 DIAMINOBENZENE; PHENYLENEDIAMINE DERIVATIVE; QUERCITRIN; RUTOSIDE; UNCLASSIFIED DRUG;

22RV1 CELL LINE; ABTS RADICAL SCAVENGING ASSAY; ANTIOXIDANT ACTIVITY; ARTICLE; AUTOANALYSIS; AUTOMATED ASSAY; DPPH RADICAL SCAVENGING ASSAY; DRUG POTENCY; DRUG SCREENING; FERRIC REDUCING ANTIOXIDANT POWER ASSAY; HUMAN; HUMAN CELL; OXIDATIVE STRESS; PC 3 CELL LINE; PIPETTE; PNT1A CELL LINE; PROSTATE CANCER CELL LINE; ROBOTICS; SPECTROPHOTOMETRY;

EID: 84871155727     PISSN: 1214021X     EISSN: 12140287     Source Type: Journal    
DOI: 10.2478/v10136-012-0006-y     Document Type: Article

References (20)

1. Damien Dorman, HJ, Shikov, AN, Pozharitskaya, ON, Hiltunen, R, Antioxidant and pro-oxidant evaluation of a Potentilla alba L. rhizome extract. Chem Biodivers 8 (2011), 1344–1356.
2. Duplancic, D, Kukoc-Modun, L, Modun, D, Radic, N, Simple and rapid method for the determination of uric acid-independent antioxidant capacity. Molecules 16 (2011), 7058–7068.
3. Gulcin, I, Measurement of antioxidant ability of melatonin and serotonin by the DMPD and CUPRAC methods as trolox equivalent. J Enz Inhib Med Chem 23 (2008), 871–876.
4. Gulcin, I, Bursal, E, Sehitoglu, MH, Bilsel, M, Goren, AC, Polyphenol contents and antioxidant activity of lyophilized aqueous extracts of propolis from Erzurum, Turkey. Food Chem Toxicol 48 (2010), 2227–2238.
5. Kaviarasan, K, Kalaiarasi, P, Pugalendi, V, Antioxidant efficacy of flavonoid-rich fraction from Spermacoce hispida in hyperlipidemic rats. J Appl Biomed 6 (2008), 165–176.
6. Kizek, R, Adam, V, Hrabeta, J, Eckschlager, T, Smutny, S, Burda, JV, Frei, E, Stiborova, M, Anthracyclines and ellipticines as DNA-damaging anticancer drugs; Recent advances. Pharmacol Ther 133 (2012), 26–39.
7. Müller, L, Fröhlich, K, Böhm, V, Comparative antioxidant activities of carotenoids measured by ferric reducing antioxidant power (FRAP), ABTS bleaching assay (alpha TEAC), DPPH assay and peroxyl radical scavenging assay. Food Chem 129 (2011), 139–148.
8. Ou, B, Huang, D, Hampsch-Woodill, M, Flanagan, JA, Deemer, EK, Analysis of antioxidant activities of common vegetables employing oxygen radical absorbance capacity (ORAC) and ferric reducing antioxidant power (FRAP) assays: a comparative study. J Agric Food Chem 50 (2002), 3122–3128.
9. Parejo, I, Codina, C, Petrakis, C, Kefalas, P, Evaluation of scavenging activity assessed by Co(II)/EDTA-induced luminol chemiluminescence and DPPH (2,2-diphenyl-1-picrylhydrazyl) free radical assay. J Pharmacol Toxicol Meth 44 (2000), 507–512.
10. Pohanka, M, Alzheimer's disease and related neurodegenerative disorders: implication and counteracting of melatonin. J Appl Biomed 9 (2011), 185–196.
11. Pohanka, M, Bandouchová, H, Vlčková, K, Žd’árová Karasová, J, Kuča, K, Damková, V, Pecková, V, Vitula, F, Pikula, J, Square wave voltammetry on screen printed electrodes: comparison to ferric reducing antioxidant power in plasma from model laboratory animal (Grey Partridge) and comparison to standard antioxidants. J Appl Biomed 9 (2011), 103–109.
12. Re, R, Pellegrini, N, Preteggente, A, Pannala, A, Yang, M, Rice-Evans, C, Antioxidant activity applying an improvement ABTS radical cation decolorization assay. Free Radic Biol Med 26 (1999), 1231–1237.
13. Salehi, P, Sonboli, A, Khaligh, P, Mirzajani, F, Essential oil composition and antioxidant activity of differential extracts of Nepeta betonicifolia C.A. Meyer and Nepeta saccharata Bunge. Nat Prod Res 26 (2012), 736–743.
14. Sochor, J, Ryvolova, M, Krystofova, O, Salas, P, Hubalek, J, Adam, V, Trnkova, L, Havel, L, Beklova, M, Zehnalek, J, Provaznik, I, Kizek, R, Fully automated spectrometric protocols for determination of antioxidant activity: advantages and disadvantages. Molecules 15 (2010), 8618–8640.
15. Sochor, J, Skutkova, H, Babula, P, Zitka, O, Cernei, N, Rop, O, Krska, B, Adam, V, Provaznik, I, Kizek, R, Mathematical evaluation of the amino acid and polyphenol content and antioxidant activities of fruits from different apricot cultivars. Molecules 16 (2011), 7428–7457.
16. Stiborová, M, Rupertová, M, Frei, E, Cytochrome P450-and peroxidase-mediated oxidation of anticancer alkaloid ellipticine dictates its anti-tumor efficiency. Biochim Biophys Acta Proteins Proteomics 1814 (2011), 175–185.
17. Votruba, M, Stopka, P, Hroudová, J, Veselý, K, Nejedlová, L, A simple method for quantitative estimation of free radicals in serum. Klin Biochem Metab 7 (1999), 96–101.
18. Zanella, A, Gandin, V, Porchia, M, Refosco, F, Tisato, F, Sorrentino, F, Scutari, G, Rigobello, MP, Marzano, C, Cytotoxicity in human cancer cells and mitochondrial dysfunction induced by a series of new copper(I) complexes containing tris(2-cyanoethyl)phosphines. Invest New Drugs 29 (2011), 1213–1223.
19. Bouayed, J, Hoffmann, L, Bohn, T, Antioxidative mechanisms of whole-apple antioxidants employing different varieties from Luxembourg. J Med Food 14 (2011), 1631–1637.
20. Zong D, Haag P, Yakymovych I, Lewensohn R, Viktorsson K. Chemosensitization by phenothiazines in human lung cancer cells: impaired resolution of gamma H2AX and increased oxidative stress elicit apoptosis associated with lysosomal expansion and intense vacuolation. Cell Death Dis. 2: e181.