Influence of the spinning rate in the HR-MAS pattern of mobile lipids in C6 glioma cells and in artificial oil bodies

Magnetic Resonance Materials in Physics, Biology and Medicine

Volumn 25, Issue 6, 2012, Pages 487-496

  Martín Sitjar, Juana ^a,b   Delgado Goñi, Teresa ^a,b   Cabañas, Miquel E ^a,b   Tzen, Jason ^c   Arús, Carles ^a,b  

^a UNIVERSITAT AUTÒNOMA DE BARCELONA   (Spain)

^b BIOMATERIALES Y NANOMEDICINA CIBER BBN   (Spain)

^c NATIONAL CHUNG HSING UNIVERSITY   (Taiwan)

Author keywords

Artificial oil bodies; Lipid droplets; Nuclear magnetic resonance

Indexed keywords

CALCIUM BINDING PROTEIN; CALEOSIN PROTEIN, SESAMUM INDICUM; FATTY ACID; LIPID; OIL; OLEOSIN PROTEIN, SESAMUM INDICUM; VEGETABLE PROTEIN;

ANIMAL; ARTICLE; CELL SURVIVAL; CHEMISTRY; CYTOSOL; EQUIPMENT; GLIOMA; METABOLISM; METHODOLOGY; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; PATHOLOGY; RAT; TEMPERATURE; TUMOR CELL LINE;

ANIMALS; CALCIUM-BINDING PROTEINS; CELL LINE, TUMOR; CELL SURVIVAL; CYTOSOL; FATTY ACIDS; GLIOMA; LIPIDS; MAGNETIC RESONANCE SPECTROSCOPY; OILS; PLANT PROTEINS; RATS; TEMPERATURE;

EID: 84870947663     PISSN: 09685243     EISSN: None     Source Type: Journal    
DOI: 10.1007/s10334-012-0327-6     Document Type: Article

References (28)

1. Hakumäki JM, Kauppinen RA (2000) 1H NMR visible lipids in the life and death of cells. Trends Biochem Sci 25:357-362 (Pubitemid 30497239)
2. Chen JH, Enloe BM, Weybright P, Campbell N, Dorfman D, Fletcher CD, Cory DG, Singer S (2002) Biochemical correlates of thiazolidinedione-induced adipocyte differentiation by high-resolution magic angle spinning NMR spectroscopy. Magn Reson Med 48(4):10-602
3. Barba I, Caban?as ME, Arús C (1999) The relationship between nuclear magnetic resonance-visible lipids, lipid droplets, and cell proliferation in cultured C6 cells. Cancer Res 59:1861-1868 (Pubitemid 29186158)
4. Mountford CE, Wright LC (1988) Organization of lipids in the plasma membranes of malignant and stimulated cells: a new model. Trends Biochem Sci 13:172-177
5. Rémy C, Fouilhe N, Barba I, Sam-Lai E, Lahrech H, Cucurella MG, Izquierdo M, Moreno A, Ziegler A, Massarelli R, Décorps M, Arús C (1997) Evidence that mobile lipids detected in rat brain glioma by 1H nuclear magnetic resonance correspond to lipid droplets. Cancer Res 57:407-414 (Pubitemid 27081656)
6. Quintero M, Caban?as ME, Arús C (2007) A possible cellular explanation for theNMR-visible mobile lipid (ML) changes in culturedC6 glioma cells with growth. Biochim Biophys Acta 177:31-44 (Pubitemid 46073880)
7. Delikatny EJ, Chawla S, Leung DJ, Poptani H (2011) MR-visible lipids and the tumor microenviroment. NMR Biomed 24(6): 592-611
8. Zweytick D, Athesntaedt K, Daum G (2000) Intracellular lipid particles of eukaryotic cells. Biochim Biophys Acta 1469: 101-120
9. Martin S, Parton RG (2006) Lipid droplets: a unified view of a dynamic organelle. Nat Rev Mol Cell Bio 7:373-378
10. Pérez Y, Lahrech H, Caban?as ME, Barnadas R, Sabés M, Rémy C, Arús C (2002) Measurement by nuclear magnetic resonance diffusion of the dimensions of the mobile lipid compartment in C6 cells. Cancer Res 62:5672-5677 (Pubitemid 35204720)
11. Beckonert O, Coen M, Keun HC, Wang Y, Ebbels TMD, Holmes E, Lindon JC, Nicholson JK (2010) High-resolution magic-anglespinning NMR spectroscopy for metabolic profiling of intact tissues. Nat Protoc 5:1019-1032
12. Lindon JC, Beckonert OP, Holmes E, Nicholson JK (2009) Highresolution magic angle spinning NMR spectroscopy: application to biomedical studies. Prog Nucl Mag Res Sp 55(2):79-100
13. Marti'n-Sitjar J, Delgado-Gon?i T, Valverde D, Caban?as, M, Nolis, P, Aru's C (2008) Unveiling the ''invisible'' neutral lipid pool in cultured log phase C6 cells using HR-MAS at 37 -C. In: Proceedings of the 24th annual meeting, European Society for Magnetic Resonance in Medicine and Biology, pp 181-182
14. Traikia M, Langlais DB, Cannarozzi GM, Devaux PF (1997) High-Resolution spectra of liposomes using MAS NMR. The case of intermediate-size vesicles. J Magn Reson 125:140-144 (Pubitemid 127451360)
15. Chen MCM, Chyan CL, Lee TTT, Huang SH, Tzen JTC (2004) Constitution of stable artificial oil bodies with triacylglycerol, phospholipids and caleosin. J Agric Food Chem 52:3982-3987
16. Tai SSK, Chen MCM, Peng CC, Tzen JTC (2002) Gene family of oleosin isoforms and their structural stabilization in sesame seed oil bodies. Biosci Biotechnol Biochem 66(10):2146-2153 (Pubitemid 39251216)
17. Peng CC, Lin IP, Lin CK, Tzen JTC (2003) Size and stability of reconstituted sesame oil bodies. Biotechnol Progr 19(5): 1623-1626 (Pubitemid 37250057)
18. Tzen JTC, Huang AHC (1992) Surface structure and properties of plant seed oil bodies. J Cell Biol 117(2):327-335
19. Peng CC, Chen JCF, Shyu DJH, Chen MJ, Tzen JTC (2004) A system for purification of recombinant proteins in Escherichia coli via artificial oil bodies constituted with their oleosin-fused polypeptides. J Biotechnol 111:51-57 (Pubitemid 38757820)
20. Liu TH, Chyan CL, Li FY, Tzen JT (2009) Stability of artificial oil bodies constituted with recombinant caleosins. J Agric Food Chem 57:2308-2313
21. Vuorela T, Catte A, Niemelä PS, Hall A, Hyvo'nen MT, Marrink SJ, Kattunen M, Vattulainen I (2010) Role of lipids in spheroidal high density lipoproteins. PLoS Comp Biol 6(10):e1000964
22. Hevonoja T, Pentikäinen MO, Hyvönen MT, Kovanen PT, Ala-Korpela M (2000) Structure of low density lipoprotein (LDL) particles: basis for understanding molecular changes in modified LDL. Biochem Biophys Acta 1488(3):189-210
23. Valverde-Saubí D, Candiota AP, Molins MA, Feliz M, Godino O, Dávila M, Acebes JJ, Arús C (2010) Short-term temperature effect on the HRMAS spectra of human brain tumor biopsies and their pattern recognition analysis. Magn Reson Mater Phy 23(4):203-215
24. Opstad KS, Bell BA, Griffiths JR, Howe FA (2008) An assessment of the effects of sample ischaemia and spinning time on the metabolic profile of brain tumour biopsy specimens as determined by high-resolution magic angle spinning 1H NMR. NMR Biomed 21:1138-1147
25. Deese AJ, Dratz EA, Hymel L, Fleischer S (1982) Proton NMR T1, T2, and T1 rho relaxation studies of native and reconstituted sarcoplasmic reticulum and phospholipid vesicles. Biophys J 37(1):16-207
26. Shapiro YE (1988) The paramagnetic hydrophilic probing of colloidal systems by NMR spectroscopy. Russ Chem Rev 57:1253-1272
27. Delikatny EJ, Cooper WA, Brammah S, Sathasivam N, Rideout DC (2002) Nuclear magnetic resonance-visible lipids induced by cationic lipophilic chemotherapeutic agents are accompanied by increased lipid droplet formation and damaged mitochondria. Cancer Res 62(5):1394-1400 (Pubitemid 34407798)
28. Griffin JL, Lehtimäki KK, Valonen PK, Gröhn OHJ, Kettunen MI, Ylä-Herttuala S, Pitkänen A, Nicholson JK, Kauppinen RA (2003) Assignment of 1H nuclear magnetic resonance visible polyunsaturated fatty acids in BT4C gliomas undergoing ganciclovir-thymidine kinase gene therapy-induced programmed cell death. Cancer Res 63:3195-3201 (Pubitemid 36735872)