The relationship between nuclear magnetic resonance-visible lipids, lipid droplets, and cell proliferation in cultured C6 cells

Cancer Research

Volumn 59, Issue 8, 1999, Pages 1861-1868

  Barba, Ignasi ^a   Cabañas, Miquel E ^a   Arús, Carles ^a  

^a UNIVERSITAT AUTÒNOMA DE BARCELONA   (Spain)

Author keywords

[No Author keywords available]

Indexed keywords

ANIMAL CELL; ARTICLE; BRAIN TUMOR; CELL PROLIFERATION; DIAGNOSTIC VALUE; LIPID ANALYSIS; NONHUMAN; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; PRIORITY JOURNAL; RAT; SOLID TUMOR; TUMOR CELL CULTURE;

ANIMALS; CELL CYCLE; CELL DIVISION; CELLS, CULTURED; CULTURE MEDIA; FLOW CYTOMETRY; GLIOMA; HYDROGEN-ION CONCENTRATION; LIPID METABOLISM; MAGNETIC RESONANCE SPECTROSCOPY; MICROSCOPY, FLUORESCENCE; OXAZINES; RATS;

EID: 0344528589     PISSN: 00085472     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (49)

1. Sijens, P. E., Knopp, M. V., Brunetti, A., Wicklow, K., Alfano, B., Bachert, P., Sanders, J. A., Stillman, A. E., Kett, H., Sauter, R., and Oudkerk, M. 1H NMR spectroscopy in patients with metastatic brain tumors: a multicenter study. Magn. Res. Med., 33: 818-826, 1995.
2. Sijens, P. E., Levendag, P. C., Vecht, C. J., Van Dijk, P., and Oudkerk, M. 1H MR spectroscopy detection of lipids and lactate in metastatic brain tumors. NMR Biomed., 9: 65-71, 1996.
3. Shimitzu, H., Kumabe, T., Tominaga, T., Katayama, T., Hara, K., Ono, Y., Sato, K., Arai, N., Fujiwara, S., and Yoshimoto, T. Noninvasive evaluation of malignancy of brain tumors with proton MR spectroscopy. Am. J. Neuroradiol., 17: 737-747, 1996.
4. Mader, I., Roser, W., Hagberg, G., Schneider, M., Sauter, R., Seeling, J., Raude, E. W., and Steinbrich, W. Proton chemical shift imaging, metabolic maps, and single voxel spectroscopy of glial brain tumors. MAGMA, 4: 139-150, 1996.
5. Negendank, W. G., Sauter, R., Brown, T. R., Evelhoch, J., Falini, A., Gotsis, E. D., Heerschap, A., Kamada, K., Lee, B. C. P., Mengeot, M. M., Moser, E., Padavic-Shaller, K. A., Sanders, J. A., Spraggins, T. A., Stillman, A. E., Terwey, B. Vogl, T. J., Wicklow, K., and Zimmerman, R. A. Proton magnetic resonance spectroscopy in patients with glial tumors: a multicenter study. J. Neurosurg., 84: 449-458, 1996.
6. Usenius, J-P., Tuohimetsa, S., Vainio, P., Ala-Korpela, M., Hiltunen, Y., and Kauppinen, R. Automated classification of human brain tumours by neural network analysis using in vivo 1H magnetic resonance spectroscopic metabolite phenotypes. Neuroreport, 7: 1597-1600, 1996.
7. Preul, M. C., Caramanos, Z., Collins, L. D., Villemure, J-G., Leblanc, R., Olivier, A., Pokrupa, R., and Arnold, D. L. Accurate, noninvasive diagnosis of human brain tumors by using proton magnetic resonance spectroscopy. Nat. Med., 2: 323-325, 1996.
8. 1H NMR spectra from brain tumours. NMR Biomed., 11: 177-191, 1998.
9. 1H MRS of high grade astrocytomas: mobile lipid accumulation in necrotic tissue. NMR Biomed., 7: 149-155, 1994.
10. 1H nuclear magnetic resonance correspond to lipid droplets. Cancer Res., 57; 404-414, 1997.
11. 1H NMR spectroscopy in rat glioma arose from large size lipid droplets in necrosis. Proceedings of the AACR Special Conference in Cancer Research: Cancer of the Central Nervous System. San Diego, June 7-11, 1997, pp. B13. Philadelphia: AACR, 1997.
12. Fouilhé, N. Caractérisation des lipides mobiles détectés par spectroscopie RMN du proton dans un modèle de gliome intracérebral chez le rat. PhD Thesis, Université. Joseph Fourier, Grenoble, France, 1997.
13. 1H MRS in vivo pattern. Program of the International Society for Magnetic Resonance in Medicine Fourth Annual Meeting, New York, April 27-May 3, 1996, pp. 976.
14. 1H MR spectroscopy. NMR Biomed., 7: 172-180, 1994.
15. Mountford, C. E., Grossman, G., Reid, G., and Fox, R. M. Characterisation of transformed cells and tumours by proton nuclear magnetic resonance spectroscopy. Cancer Res., 42: 2270-2276, 1982.
16. Mountford, C. E., and Tattersall, M. H. N. Proton magnetic resonance spectroscopy and tumor detection. Cancer Surv., 6: 285-314, 1987.
17. Mountford, C. E., and Wright, L. C. Organisation of lipids in the plasma membranes of malignant and stimulated cells: a new model. Trends Biochem. Sci., 13: 172-177, 1988.
18. 1H magnetic resonance signals form malignant cells do rot arise from intracellular lipid droplets. NMR Biomed., 2; 161-164, 1989.
19. 1H NMR spectra of a myeloma cell line correlates with the induced formation of cytoplasmic lipid droplets. Magn. Res. Med., 29; 564-550, 1993.
20. Delikatny, E. J., Lander, C. M., Jeitner, T. M., Hancock, R., and Mountford, C. E. Modulation of MR-visible lipid levels by cell culture conditions and correlations with chemotactic response. Int. J. Cancer, 65: 238-245, 1996.
21. Delikatny, E. J., Roman, S. K., Hancock, R., Jeitner, T. M., Lander, C. M., Rideout, D. C., and Mountford, C. E. Tetraphenylphosphonium chloride induced MR-visible lipid accumulation in a malignant human breast cell line. Int. J. Cancer, 67: 72-79, 1996.
22. Le Moyec, L., Tatoud, R., Degorges, A., Calabresse, C., Bauza, G., Eugène, M., and Calvo, F. Proton nuclear magnetic resonance speclroscopy reveals cellular lipids involved in resistance to Adriamycin and Taxol by the K562 leukaemia cell line. Cancer Res., 56: 3461-3467, 1996.
23. Le Moyec, L., Millot, G., Tatoud, R., Calvo, F., and Eugène, M. Lipid signals detected by NMR proton spectroscopy of whole cells are not correlated to lipid droplets evidence by the nile red staining. Cell. Mol. Biol., 43: 703-709, 1997.
24. 1H MRS-visible mobile lipids in H-ras transformed tumorigenic fibroblast with respect to their untransformed parental cells. Cell. Mol. Biol., 43: 691-701, 1997.
25. Delikatny, E. J., Russel, P., Hunter, C., Hancock, R., Atkinson, K. H., van Haaften-Day, C., and Mountford, C. E. Proton MR and human cervical neoplasia: ex vivo spectroscopy allows distinction of invasive carcinoma of the cervix from carcinoma in situ and other preinvasive lesions. Radiology, 188: 791-796, 1993.
26. Benda, P., Lightbody, J., Sato, G., Levine, L., and Sweet, W. Differentiated rat glial cell strain in tissue culture. Science (Washington DC), 161: 370-371, 1968.
27. Benda, P., Someda, K., Messer, J., and Sweet, W. Morphological and immunochemical studies of rat glial tumors and clonal strains propagated in culture. J. Neurosurg., 34: 310-323, 1971.
28. Rémy, C., Arús, C., Ziegler, A., Sam Laï, E., Moreno, A., and Décorps, M. In ; vivo, ex vivo and in vitro one- and two-dimensional nuclear magnetic resonance spectroscopy of an intracerebral glioma in rat brain: assignment of resonances. J. Neurochem., 62: 166-179, 1994.
29. Bradford, M. M. A rapid and sensitive method for quantitation of microgram quantities of protein utilising the principle of protein-dye binding. Anal. Biochem., 72: 248-254, 1976.
30. Hahn, E. L. Spin echoes. Phys. Rev., 80: 580-594, 1950.
31. Plateau, P., and Guéron, M. Exchangeable proton NMR without base-line distortion using new strong-pulse sequences. J. Am. Chem. Soc., 104: 7310-7311, 1982.
32. Moreno, A. Recerca i caracterització de marcadors tumorals mitjançant l'espectroscòpia de ressonància magnètica nuclear de proró en el cas del càncer del budell gruixut, PhD Thesis, Universitat Autònoma de Barcelona, 1997.
33. Greenspan, P., Mayer, E. P., and Fowler, S. D. Nile red: a selective fluorescent stain for intracellular lipid droplets. J. Cell Biol., 100: 965-973, 1985.
34. Moreton, K., Turner, R., Blake, N., Paton, A., and Rumsby, M. Protein expression of the α, γ, δ and ∈ subspecies of protein kinase C changes as C6 glioma cells become contact inhibited and quiescent in the presence of serum. FEBS Lett., 372: 33-38, 1995.
35. Karasaki, S. Passage of cytoplasmic lipid into interphase nuclei in preneoplastic rat liver. J. Ultrastruct. Res., 42: 463-478, 1973.
36. 1H NMR, Nile red and electron microscopy analyses of mobile neutral lipids in N1H-3T3 fibroblasts and their H-ras transformed cell variants. Program of the International Society for Magnetic Resonance in Medicine Sixth Annual Meeting, Sydney, Australia, April 18-24, 1998, p. 333.
37. Freitas, I., Pontiaggia, P., Barni, S., Bertone V., Parente, M., Novarina, A., Roveta, G., Gerzeli, G., and Stoward, P. Histochemical probes for the detection of hypoxic tumor cells. Anticancer Res., 10: 613-622, 1990.
38. Wenner, C. E., and Tomei, L. D. Phenotypic expression of malignant transformation and its relationship to energy metabolism. In: I. L. Cameron and B. P. Thomas (eds.), The transformed cell, pp. 163-188. New York: Academic Press, 1981.
39. Gocze, P. M., and Freeman, D. A. Factors underlying the variability of lipid droplet fluorescence in MA-10 leydig tumor cells. Cytometry, 17: 151-158, 1994.
40. Hirakawa, T., Maruyama, K., Kohl, N. E., and Kodoma, T. Massive accumulation of neutral lipids in cells conditionally transformed by an activated H-ras oncogene. Oncogene, 6: 289-295, 1991.
41. Jackowski, S. Coordination of membrane phospholipid synthesis with the cell cycle. J. Biol. Chem., 269: 3858-3867, 1994.
42. Jackowski, S. Cell cycle regulation of membrane phospholipid metabolism. J. Biol. Chem., 271: 20219-20222, 1996.
43. Roman, S. K., Jeitner, T. M., Hancock, R. H., Cooper, W. A., Rideout, D. C., and Delikatny, E. J. Induction of magnetic resonance-visible lipid in a transformed human breast cell line by tetraphenylphosphonium chloride. Int. J. Cancer, 73: 570-579, 1997.
44. Eagle, H. The effect of environmental pH on the growth of normal and malignant cells. J. Cell Physiol., 82: 1-8, 1973.
45. Bear, M. P., and Sheider, F. H. The effect of medium pH on rate of growth, neurite formation and acetylcholinesterase activity in mouse neuroblastoma cells in culture. J. Cell. Physiol., 91: 63-68, 1976.
46. 31P MR spectroscopy study using continuous bioreactor system. Magn. Res. Med., 33: 422-426, 1995.
47. Gasparovic, C., Barba, I., Born, J., Barton, S., Arús, C., and Mann, P. A study of imidazole-based NMR probes of cellular pH. Anal. Biochem., 262: 64-72, 1998.
48. Griffiths, J. R. Are cancer cells acidic? Br. J. Cancer, 64: 425-427, 1991.
49. Jähde, E., and Rajewsky, M. F. Tumor-selective modification of cellular microenvironment in vivo: effect of glucose infusion on the pH in normal and malignant rat tissues. Cancer Res., 42: 1505-1512, 1982.