Preserving the morphology and evaluating the quality of liver grafts by hypothermic machine perfusion: A proof-of-concept study using discarded human livers

Liver Transplantation

Volumn 18, Issue 12, 2012, Pages 1495-1507

  Monbaliu, Diethard ^a   Liu, Qiang ^a   Libbrecht, Louis ^c   De Vos, Rita ^b   Vekemans, Katrien ^b   Debbaut, Charlotte ^d   Detry, Olivier ^e   Roskams, Tania ^b   Van Pelt, Jos ^b   Pirenne, Jacques ^a  

^a REGA INSTITUTE   (Belgium)

^b UNIVERSITY OF LEUVEN   (Belgium)

^c GHENT UNIVERSITY HOSPITAL   (Belgium)

^d GHENT UNIVERSITY   (Belgium)

^e UNIVERSITY OF LIÈGE   (Belgium)

Author keywords

[No Author keywords available]

Indexed keywords

ASPARTATE AMINOTRANSFERASE; LACTATE DEHYDROGENASE;

ADOLESCENT; ADULT; AGED; ARTICLE; CONTROLLED STUDY; EVALUATION; FEMALE; HUMAN; HUMAN TISSUE; HYPOTHERMIC MACHINE PERFUSION; LIVER GRAFT; LIVER PERFUSION; LIVER PRESERVATION; MACHINE; MALE; MORPHOLOGY; PARAMETERS; PRIORITY JOURNAL; QUALITY CONTROL;

ADENOSINE TRIPHOSPHATE; ADULT; AGED; AGED, 80 AND OVER; ALANINE TRANSAMINASE; ASPARTATE AMINOTRANSFERASES; BIOLOGICAL MARKERS; CARBON DIOXIDE; COLD TEMPERATURE; EQUIPMENT DESIGN; FEMALE; GENE EXPRESSION REGULATION; HUMANS; HYDROGEN-ION CONCENTRATION; L-LACTATE DEHYDROGENASE; LACTIC ACID; LIVER; LIVER TRANSPLANTATION; MALE; MIDDLE AGED; ORGAN PRESERVATION; ORGAN PRESERVATION SOLUTIONS; OXYGEN; PARTIAL PRESSURE; PERFUSION; RNA, MESSENGER; TIME FACTORS; TISSUE DONORS; VASCULAR RESISTANCE; YOUNG ADULT;

EID: 84870892714     PISSN: 15276465     EISSN: 15276473     Source Type: Journal    
DOI: 10.1002/lt.23550     Document Type: Article

References (29)

1. Wolfe RA, Merion RM, Roys EC, Port FK,. Trends in organ donation and transplantation in the United States, 1998-2007. Am J Transplant 2009; 9 (pt 2): 869 -878.
2. Durand F, Renz JF, Alkofer B, Burra P, Clavien PA, Porte RJ, et al. Report of the Paris consensus meeting on expanded criteria donors in liver transplantation. Liver Transpl 2008; 14: 1694 -1707.
3. Moers C, Varnav OC, van Heurn E, Jochmans I, Kirste GR, Rahmel A, et al. The value of machine perfusion perfusate biomarkers for predicting kidney transplant outcome. Transplantation 2010; 90: 966 -973.
4. Jochmans I, Moers C, Smits JM, Leuvenink HG, Treckmann J, Paul A, et al. The prognostic value of renal resistance during hypothermic machine perfusion of deceased donor kidneys. Am J Transplant 2011; 11: 2214 -2220.
5. Guarrera JV, Henry SD, Samstein B, Odeh-Ramadan R, Kinkhabwala M, Goldstein MJ, et al. Hypothermic machine preservation in human liver transplantation: the first clinical series. Am J Transplant 2010; 10: 372 -381.
6. de Rougemont O, Breitenstein S, Leskosek B, Weber A, Graf R, Clavien PA, Dutkowski P,. One hour hypothermic oxygenated perfusion (HOPE) protects nonviable liver allografts donated after cardiac death. Ann Surg 2009; 250: 674 -683.
7. Vekemans K, Liu Q, Brassil J, Komuta M, Pirenne J, Monbaliu D,. Influence of flow and addition of oxygen during porcine liver hypothermic machine perfusion. Transplant Proc 2007; 39: 2647 -2651.
8. 't Hart NA, der van Plaats A, Leuvenink HG, van Goor H, Wiersema-Buist J, Verkerke GJ, et al. Determination of an adequate perfusion pressure for continuous dual vessel hypothermic machine perfusion of the rat liver. Transpl Int 2007; 20: 343 -352.
9. 't Hart NA, van der Plaats A, Faber A, Leuvenink HG, Olinga P, Wiersema-Buist J, et al. Oxygenation during hypothermic rat liver preservation: an in vitro slice study to demonstrate beneficial or toxic oxygenation effects. Liver Transpl 2005; 11: 1403 -1411.
10. Minor T, Koetting M, Koetting M, Kaiser G, Efferz P, Lüer B, Paul A,. Hypothermic reconditioning by gaseous oxygen improves survival after liver transplantation in the pig. Am J Transplant 2011; 11: 2627 -2634.
11. Franzén LE, Ekstedt M, Kechagias S, Bodin L,. Semiquantitative evaluation overestimates the degree of steatosis in liver biopsies: a comparison to stereological point counting. Mod Pathol 2005; 18: 912 -916.
12. Spitzer AL, Lao OB, Dick AA, Bakthavatsalam R, Halldorson JB, Yeh MM, et al. The biopsied donor liver: incorporating macrosteatosis into high-risk donor assessment. Liver Transpl 2010; 16: 874 -884.
13. Monbaliu D, Libbrecht L, De Vos R, Vekemans K, Walter H, Liu Q, et al. The extent of vacuolation in non-heart-beating porcine donor liver grafts prior to transplantation predicts their viability. Liver Transpl 2008; 14: 1256 -1265.
14. Moers C, Smits JM, Maathuis MH, Treckmann J, van Gelder F, Napieralski BP, et al. Machine perfusion or cold storage in deceased-donor kidney transplantation. N Engl J Med 2009; 360: 7 -19.
15. Wight J, Chilcott J, Holmes M, Brewer N,. The clinical and cost-effectiveness of pulsatile machine perfusion versus cold storage of kidneys for transplantation retrieved from heart-beating and non-heart-beating donors. Health Technol Assess 2003; 7: 1 -94.
16. Pienaar BH, Lindell SL, Van Gulik T, Southard JH, Belzer FO,. Seventy-two-hour preservation of the canine liver by machine perfusion. Transplantation 1990; 49: 258 -260.
17. Monbaliu D, Crabbé T, Roskams T, Fevery J, Verwaest C, Pirenne J,. Livers from non-heart-beating donors tolerate short periods of warm ischemia. Transplantation 2005; 79: 1226 -1230.
18. Liu Q, Monbaliu D, Vekemans K, Heedfeld V, Wylin T, Brassil J, Pirenne J, van Pelt J,. Release of AST and LFABP from ischemically damaged livers during machine perfusion: a new tool to predict viability and primary-non-function [abstract]. Transpl Int 2009; 22 (Supplement 2) 55.
19. Derveaux K, Monbaliu D, Crabbé T, Schein D, Brassil J, Kravitz D, et al. Does ex vivo vascular resistance reflect viability of non-heart-beating donor livers? Transplant Proc 2005; 37: 338 -339.
20. Monbaliu D, Vekemans K, De Vos R, Brassil J, Heedfeld V, Qiang L, et al. Hemodynamic, biochemical, and morphological characteristics during preservation of normal porcine livers by hypothermic machine perfusion. Transplant Proc 2007; 39: 2652 -2658.
21. Liu Q, Vekemans K, van Pelt J, Pirenne J, Himmelreich U, Heedfeld V, et al. Discriminate liver warm ischemic injury during hypothermic machine perfusion by proton magnetic resonance spectroscopy: a study in a porcine model. Transplant Proc 2009; 41: 3383 -3386.
22. Liu Q, Monbaliu D, Vekemans K, Peeters R, De Keyzer F, Dresselaers T, et al. Can apparent diffusion coefficient discriminate ischemic from nonischemic livers? A pilot experimental study. Transplant Proc 2007; 39: 2643 -2646.
23. Tulipan JE, Stone J, Samstein B, Kato T, Emond JC, Henry SD, Guarrera JV,. Molecular expression of acute phase mediators is attenuated by machine preservation in human liver transplantation: preliminary analysis of effluent, serum, and liver biopsies. Surgery 2011; 150: 352 -360.
24. Guarrera JV, Henry SD, Chen SW, Brown T, Nachber E, Arrington B, et al. Hypothermic machine preservation attenuates ischemia/reperfusion markers after liver transplantation: preliminary results. J Surg Res 2011; 167: e365 -e373.
25. Vogel T, Brockmann JG, Friend PJ,. Ex-vivo normothermic liver perfusion: an update. Curr Opin Organ Transplant 2010; 15: 167 -172.
26. De Gottardi A, Vinciguerra M, Sgroi A, Moukil M, Ravier-Dall'Antonia F, Pazienza V, et al. Microarray analyses and molecular profiling of steatosis induction in immortalized human hepatocytes. Lab Invest 2007; 87: 792 -806.
27. Angele MK, Rentsch M, Hartl WH, Wittmann B, Graeb C, Jauch KW, Loehe F,. Effect of graft steatosis on liver function and organ survival after liver transplantation. Am J Surg 2008; 195: 214 -220.
28. El-Badry AM, Breitenstein S, Jochum W, Washington K, Paradis V, Rubbia-Brandt L, et al. Assessment of hepatic steatosis by expert pathologists: the end of a gold standard. Ann Surg 2009; 250: 691 -697.
29. Vekemans K, van Pelt J, Komuta M, Wylin T, Heedfeld V, Detry O, et al. Attempt to rescue discarded human liver grafts by end ischemic hypothermic oxygenated machine perfusion. Transplant Proc 2011; 43: 3455 -3459.