Application of second-derivative fluorescence spectroscopy to monitor subtle changes in a monoclonal antibody structure

Journal of Pharmaceutical Sciences

Volumn 102, Issue 1, 2013, Pages 52-61

  Abbas, Shermeen A ^a   Gaspar, Greta ^a   Sharma, Vikas K ^b   Patapoff, Thomas W ^b   Kalonia, Devendra S ^a  

^a UNIVERSITY OF CONNECTICUT   (United States)

^b GENENTECH INC   (United States)

Author keywords

Excipients; Fluorescence spectroscopy; Monoclonal antibodies; Polyols; Protein aggregation; Protein structure; Protein unfolding; Proteins; Second derivative; Tryptophan

Indexed keywords

BUFFER; ETHYLENE GLYCOL; MONOCLONAL ANTIBODY; POLYOL; SUCROSE; TRYPTOPHAN; UREA;

ANTIBODY STRUCTURE; ARTICLE; CHEMICAL STRESS; DENATURATION; FLUORESCENCE SPECTROSCOPY; IONIC STRENGTH; PH; PROTEIN TERTIARY STRUCTURE; PROTEIN UNFOLDING; TEMPERATURE STRESS;

EID: 84870709849     PISSN: 00223549     EISSN: 15206017     Source Type: Journal    
DOI: 10.1002/jps.23354     Document Type: Article

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