Second derivative fluorescence spectroscopy of tryptophan in proteins

Journal of Biochemical and Biophysical Methods

Volumn 50, Issue 2-3, 2002, Pages 163-178

  Mozo Villarías, A ^a  

^a Universitat de Lleida Facultat de Medicina Sabadell   (Spain)

Author keywords

Hydrophobicity; Protein fluorescence; Second derivative spectrofluorimetry; Tryptophan fluorescence

Indexed keywords

ALBUMIN; MELITTIN; N ACETYLTRYPTOPHAN; PROTEIN; SOLVENT; TISSUE EXTRACT; TRYPTOPHAN; TYROSINE;

ARTICLE; FLUORESCENCE SPECTROSCOPY; HUMAN; HYDROPHOBICITY; NONHUMAN; PRIORITY JOURNAL; PRODUCTIVITY; PROTEIN ANALYSIS; TURBIDITY;

ANIMALS; CATTLE; HUMANS; MELITTEN; PROTEINS; SERUM ALBUMIN; SERUM ALBUMIN, BOVINE; SOLVENTS; SPECTROMETRY, FLUORESCENCE; TRYPTOPHAN; TYROSINE;

BOVINAE;

EID: 0037016375     PISSN: 0165022X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0165-022X(01)00181-6     Document Type: Article

References (21)

1. Optical method
2. UV difference spectroscopy
3. Fourth derivative spectra
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5. Fourth derivative spectrophotometry analysis of tryptophan environment in proteins
6. Thermal transitions in the structure of tubulin
7. Derivative fluorescence spectroscopy
8. Analysis of mixtures of oxytetracycline and rivoflavin by first-derivative synchronous spectrofluorimetry
9. Simultaneous determination of acetylsalicylic acid and its major metabolites in human serum by second-derivative aynchronous fluorescence spectrometry
10. New emission band by tyrosine induced by interaction with phosphate ion
11. Tyrosinate fuorescence maxima at 345 nm in proteins lacking tryptophan at pH 7
12. Quenching of tyrosine fluorescence by phosphate ions
13. Expression of a synthetic geneencoding potato carboxypeptidase inhibitor using a bacterial secretion vector
14. Agonist stimulation of phosphoinositide breakdown in brain membranes
15. b excitation bands
16. Temperature-dependence shift of fluorescence spectra without conformational changes in protein: Studies of dipolar relaxation in the melittin molecule
17. Conformational change and self-association of monomeric melittin
18. Three-dimensional structure of human serum albumin
19. Refined crystal structure of the potato inhibitor complex of carboxypeptidase A at 2.5 A resolution
20. C-tail valine is key residue for stabilization of complex between potato inhibitor and carboxypeptidase A