Single amino acid exchange in bacteriophage HK620 tailspike protein results in thousand-fold increase of its oligosaccharide affinity

Glycobiology

Volumn 23, Issue 1, 2013, Pages 59-68

  Broeker, Nina K ^a   Gohlke, Ulrich ^b   Müller, Jürgen J ^b   Uetrecht, Charlotte ^a,d,e   Heinemann, Udo ^b,c   Seckler, Robert ^a   Barbirz, Stefanie ^a  

^a UNIVERSITY OF POTSDAM   (Germany)

^b MAX DELBRÜCK CENTER FOR MOLECULAR MEDICINE   (Germany)

^c FREIE UNIVERSITÄT BERLIN   (Germany)

^d EUROPEAN XFEL GMBH   (Germany)

^e UPPSALA UNIVERSITY   (Sweden)

Author keywords

bacterial O antigen; carbohydrate interaction; site directed mutagenesis; structural thermodynamics; tailspike protein

Indexed keywords

BACTERIOPHAGE HK620 TAILSPIKE PROTEIN; CARBOHYDRATE; GLUTAMIC ACID; GLUTAMINE; MUTANT PROTEIN; O ANTIGEN; OLIGOSACCHARIDE; SOLVENT; UNCLASSIFIED DRUG; VIRUS PROTEIN;

AMINO ACID SUBSTITUTION; ARTICLE; BACTERIOPHAGE; BACTERIOPHAGE HK620; BINDING SITE; COMPLEX FORMATION; CRYSTAL STRUCTURE; DISSOCIATION CONSTANT; ENTHALPY; ESCHERICHIA COLI; HEAT; HYDROGEN BOND; HYDROPHOBICITY; ISOTHERMAL TITRATION CALORIMETRY; NONHUMAN; PRIORITY JOURNAL; PROTEIN BINDING; REACTION ANALYSIS; ROOM TEMPERATURE;

AMINO ACIDS; ASPARAGINE; ASPARTIC ACID; BINDING SITES; CATALYTIC DOMAIN; COLIPHAGES; CRYSTALLOGRAPHY, X-RAY; HYDROGEN BONDING; MODELS, MOLECULAR; O ANTIGENS; OLIGOSACCHARIDES; PROTEIN CONFORMATION; SURFACE PROPERTIES; THERMODYNAMICS; VIRAL TAIL PROTEINS;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 84870044601     PISSN: 09596658     EISSN: 14602423     Source Type: Journal    
DOI: 10.1093/glycob/cws126     Document Type: Article

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