Coupling methanol denaturation, immobilized trypsin digestion, and accurate mass and time tagging for liquid-chromatography-based shotgun proteomics of low nanogram amounts of RAW 264.7 cell lysate

Analytical Chemistry

Volumn 84, Issue 20, 2012, Pages 8715-8721

  Sun, Liangliang ^a   Zhu, Guijie ^a   Li, Yihan ^a   Yang, Ping ^a   Dovichi, Norman J ^a  

^a UNIVERSITY OF NOTRE DAME   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ACCURATE MASS; CELL LYSATES; CELL PROTEINS; IMMOBILIZED TRYPSIN; MAMMALIAN CELLS; NANOGRAMS; PEPTIDE SIGNALS; PROTEOMIC ANALYSIS; SAMPLE SIZES; SHOTGUN PROTEOMICS; STANDARD PROTEINS; TIME TAGGING; TRYPSIN DIGESTION;

CELLS; DENATURATION; LIQUID CHROMATOGRAPHY; METABOLISM; METHANOL; MOLECULAR BIOLOGY; PEPTIDES; UREA;

CYTOLOGY;

IMMOBILIZED ENZYME; METHANOL; PROTEIN; TRYPSIN;

AMINO ACID SEQUENCE; ANIMAL; ARTICLE; CELL LINE; ELECTROSPRAY MASS SPECTROMETRY; LIQUID CHROMATOGRAPHY; METABOLISM; METHODOLOGY; MOUSE; PEPTIDE MAPPING; PROTEIN DENATURATION; PROTEOMICS; TANDEM MASS SPECTROMETRY;

AMINO ACID SEQUENCE; ANIMALS; CELL LINE; CHROMATOGRAPHY, LIQUID; ENZYMES, IMMOBILIZED; METHANOL; MICE; PEPTIDE MAPPING; PROTEIN DENATURATION; PROTEINS; PROTEOMICS; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION; TANDEM MASS SPECTROMETRY; TRYPSIN;

EID: 84869476452     PISSN: 00032700     EISSN: 15206882     Source Type: Journal    
DOI: 10.1021/ac3019608     Document Type: Article

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