CZE-ESI-MS/MS system for analysis of subnanogram amounts of tryptic digests of a cellular homogenate

Proteomics

Volumn 12, Issue 19-20, 2012, Pages 3013-3019

  Sun, Liangliang ^a   Zhu, Guijie ^a   Li, Yihan ^a   Wojcik, Roza ^a,b   Yang, Ping ^a   Dovichi, Norman J ^a  

^a UNIVERSITY OF NOTRE DAME   (United States)

^b LECO Inc   (United States)

Author keywords

CZE ESI MS MS; Electrokinetically driven sheath flow interface; Protein digests; Technology

Indexed keywords

BOVINE SERUM ALBUMIN; CELL PROTEIN; PEPTIDE;

ANIMAL CELL; ARTICLE; CAPILLARY ZONE ELECTROPHORESIS; CELL LINE; CELL LYSATE; COMPUTER PROGRAM; LIMIT OF DETECTION; MASS SPECTROMETER; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; QUANTITATIVE ANALYSIS; SAMPLE SIZE; TANDEM MASS SPECTROMETRY;

ANIMALS; CATTLE; CELL EXTRACTS; CELL LINE; DATABASES, PROTEIN; ELECTROPHORESIS, CAPILLARY; LIMIT OF DETECTION; LINEAR MODELS; MICE; PEPTIDE FRAGMENTS; PEPTIDE MAPPING; REPRODUCIBILITY OF RESULTS; SERUM ALBUMIN, BOVINE; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION; TANDEM MASS SPECTROMETRY; TRYPSIN;

EID: 84867792660     PISSN: 16159853     EISSN: 16159861     Source Type: Journal    
DOI: 10.1002/pmic.201200100     Document Type: Article

References (30)

1. Krylov, S. N., Dovichi, N. J., Capillary electrophoresis for the analysis of biopolymers. Anal. Chem. 2000, 72, 111R-128R.
2. Geiger, M., Hogerton, A. L., Bowser, M. T., Capillary electrophoresis. Anal. Chem. 2012, 84, 577-596.
3. Jorgenson, J. W., Lukacs, K. D., Capillary zone electrophoresis. Science 1983, 222, 266-272.
4. Maxwell, E. J., Chen, D. D., Twenty years of interface development for capillary electrophoresis-electrospray ionization-mass spectrometry. Anal. Chim. Acta. 2008, 627, 25-33.
5. Chen, Y. R., Tseng, M. C., Her, G. R., Design and performance of a low-flow capillary electrophoresis-electrospray-mass spectrometry interface using an emitter with dual beveled edge. Electrophoresis 2005, 26, 1376-1382.
6. Liu, C. C., Zhang, J., Dovichi, N. J., A sheath-flow nanospray interface for capillary electrophoresis/mass spectrometry. Rapid Commun. Mass Spectrom. 2005, 19, 187-192.
7. Tseng, M. C., Chen, Y. R., Her, G. R., A low-makeup beveled tip capillary electrophoresis /electrospray ionization mass spectrometry interface for micellar electrokinetic chromatography and nonvolatile buffer capillary electrophoresis. Anal. Chem. 2004, 76, 6306-6312.
8. Yang, Y., Boysen, R. I., Hearn, M. T., Optimization of field-amplified sample injection for analysis of peptides by capillary electrophoresis-mass spectrometry. Anal. Chem. 2006, 78, 4752-4758.
9. Lewis, K. C., Opiteck, G. J., Jorgenson, J. W., Sheeley, D. M., Comprehensive on-line RPLC-CZE-MS of peptides. J. Am. Soc. Mass Spectrom. 1997, 8, 495-500.
10. Su, S., Yu, Y., Online preconcentration of recombinant Arg-Gly-Asp-hirudin using dynamic pH junction for analysis in human urine samples by capillary electrophoresis-mass spectrometry. J. Chromatogr A. 2009, 1216, 1490-1495.
11. Good DM, Zürbig P, Argilés A, Bauer HW et al., Naturally occurring human urinary peptides for use in diagnosis of chronic kidney disease. Mol. Cell Proteomics. 2010, 9, 2424-2437.
12. Zhong, X., Maxwell, E. J., Ratnayake, C., Mack, S. et al., Flow-through microvial facilitating interface of capillary isoelectric focusing and electrospray ionization mass spectrometry. Anal. Chem. 2011, 83, 8748-8755.
13. Smith, R. D., Barinaga, C. J., Udseth, H. R., Improved electrospray ionization interface for capillary zone electrophoresis - mass-spectrometry. Anal. Chem. 1988, 60, 1948-1952.
14. Maxwell, E. J., Zhong, X., Zhang, H., van Zeijl, N. et al., Decoupling CZE and ESI for a more robust interface with MS. Electrophoresis 2010, 31, 1130-1137.
15. Kelly, J. F., Ramaley, L., Thibault, P., Capillary zone electrophoresis-electrospray mass spectrometry at submicroliter flow rates: practical considerations and analytical performance. Anal. Chem. 1997, 69, 51-60.
16. Moini, M., Simplifying CE-MS operation. 2. Interfacing low-flow separation techniques to mass spectrometry using a porous tip. Anal. Chem. 2007, 79, 4241-4246.
17. Haselberg, R., Ratnayake, C. K., de Jong, G. J., Somsen, G. W., Performance of a sheathless porous tip sprayer for capillary electrophoresis-electrospray ionization-mass spectrometry of intact proteins. J. Chromatogr. A. 2010, 1217, 7605-7611.
18. Faserl, K., Sarg, B., Kremser, L., Lindner, H., Optimization and evaluation of a sheathless capillary electrophoresis-electrospray ionization mass spectrometry platform for peptide analysis: comparison to liquid chromatography-electrospray ionization mass spectrometry. Anal. Chem. 2011, 83, 7297-7305.
19. Wojcik, R., Li, Y., Maccoss, M. J., Dovichi, N. J., Capillary electrophoresis with Orbitrap-Velos mass spectrometry detection. Talanta 2012, 88, 324-329.
20. Wojcik, R., Dada, O. O., Sadilek, M., Dovichi, N. J., Simplified capillary electrophoresis nanospray sheath-flow interface for high efficiency and sensitive peptide analysis. Rapid Commun. Mass Spectrom. 2010, 24, 2554-2560.
21. Li, Y., Wojcik, R., Dovichi, N. J., A replaceable microreactor for on-line protein digestion in a two-dimensional capillary electrophoresis system with tandem mass spectrometry detection. J. Chromatogr. A. 2011, 1218, 2007-2011.
22. Li, Y., Champion, M. M., Sun, L., Champion, P. A. et al., Capillary zone electrophoresis-electrospray ionization-tandem mass spectrometry as an alternative proteomics platform to ultraperformance liquid chromatography-electrospray ionization-tandem mass spectrometry for samples of intermediate complexity. Anal. Chem. 2012, 84, 1617-1622.
23. Sun, L., Li, Y., Yang, P., Zhu, G. et al., High efficiency and quantitatively reproducible protein digestion by trypsin-immobilized magnetic microspheres. J. Chromatogr. A. 2012, 1220, 68-74.
24. Cohen, D., Dickerson, J. A., Whitmore, C. D., Turner, E. H. et al., Chemical cytometry: fluorescence-based single-cell analysis. Annu. Rev. Anal. Chem. (Palo Alto Calif.) 2008, 1, 165-190.
25. Hu, S., Le, Z., Krylov, S., Dovichi, N. J., Cell cycle-dependent protein fingerprint from a single cancer cell: image cytometry coupled with single-cell capillary sieving electrophoresis. Anal. Chem. 2003, 75, 3495-3501.
26. Smith, R. D., Anderson, G. A., Lipton, M. S., Pasa-Tolic, L. et al., An accurate mass tag strategy for quantitative and high-throughput proteome measurements. Proteomics 2002, 2, 513-523.
27. Gerber, S. A., Rush, J., Stemman, O., Kirschner, M. W. et al., Absolute quantification of proteins and phosphoproteins from cell lysates by tandem MS. Proc. Natl. Acad. Sci. USA 2003, 100, 6940-6945.
28. Krylov, S. N., Zhang, Z., Chan, N. W., Arriaga, E. et al. Correlating cell cycle with metabolism in single cells: combination of image and metabolic cytometry. Cytometry 1999, 37, 14-20.
29. Schoenherr, R. M., Ye, M., Vannatta, M., Dovichi, N. J., CE-microreactor-CE-MS/MS for protein analysis. Anal. Chem. 2007, 79, 2230-2238.
30. Ye, M., Hu, S., Schoenherr, R. M., Dovichi, N. J., On-line protein digestion and peptide mapping by capillary electrophoresis with post-column labeling for laser-induced fluorescence detection. Electrophoresis 2004, 25, 1319-1326.