The Streptomyces rubiginosus xylose isomerase is misfolded when expressed in Saccharomyces cerevisiae

Enzyme and Microbial Technology

Volumn 32, Issue 2, 2003, Pages 252-259

  Gárdonyi, Márk ^a   Hahn Hägerdal, Bärbel ^a  

^a LUND UNIVERSITY   (Sweden)

Author keywords

Protein folding; Saccharomyces cerevisiae; Xylose fermentation; Xylose isomerase

Indexed keywords

DETERGENTS; ENZYMES; EXTRACTION; GENES; PROTEINS;

ISOMERASE ACTIVITIES;

MICROBIOLOGY;

DETERGENT; DODECYL SULFATE SODIUM; XYLOSE ISOMERASE;

ARTICLE; CELL LYSATE; CONTROLLED STUDY; ENZYME ACTIVITY; GENE; GENE EXPRESSION; HOST; MOLECULAR CLONING; NONHUMAN; PROTEIN EXPRESSION; PROTEIN FOLDING; PROTEIN STRUCTURE; SACCHAROMYCES CEREVISIAE; STREPTOMYCES; STREPTOMYCES RUBIGINOSUS; THERMUS THERMOPHILUS; XYLA GENE;

SACCHAROMYCES CEREVISIAE; STREPTOMYCES RUBIGINOSUS; THERMUS THERMOPHILUS;

EID: 0037415332     PISSN: 01410229     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0141-0229(02)00285-5     Document Type: Article

References (47)

1. Kötter P., Amore R., Hollenberg C.P., Ciriacy M. Isolation and characterization of the Pichia stipitis xylitol dehydrogenase gene, XYL2, and construction of a xylose-utilizing Saccharomyces cerevisiae transformant. Curr. Genet. 18:1990;493-500.
2. Hallborn J., Walfridsson M., Airaksinen U., Ojamo H., Hahn-Hägerdal B., Penttilä M.et al. Xylitol production by recombinant Saccharomyces cerevisiae. Biotechnology. 9:1991;1090-1095.
3. Tantirungkij M., Nakashima N., Seki T., Yoshida T. Construction of xylose-assimilating Saccharomyces cerevisiae. J. Ferm. Bioeng. 75:1993;83-88.
4. Kötter P., Ciriacy M. Xylose fermentation by Saccharomyces cerevisiae. Appl. Microbiol. Biotechnol. 38:1993;776-783.
5. Verduyn C., van Kleef R., Frank J., Schreuder H., van Dijken J.P., Scheffers W.A. Properties of the NAD(P)H-dependent xylose reductase from the xylose-fermenting yeast Pichia stipitis. Biochem. J. 226:1985;669-677.
6. Rizzi M., Erlemann P., Bui-Thanh N.-A., Dellweg H. Xylose fermentation by yeasts 4. Purification and kinetic studies of xylose reductase from Pichia stipitis. Appl. Microbiol. Biotechnol. 29:1988;148-154.
7. Bruinenberg P.M., de Bot P.H.M., van Dijken J.P., Scheffers W.A. The role of redox balances in the anaerobic fermentation of xylose by yeast. Eur. J. Appl. Microbiol. 18:1983;287-292.
8. +-xylitol-dehydrogenase from the yeast Pichia stipitis. J. Ferm. Bioeng. 67:1989;25-30.
9. Walfridsson M., Anderlund M., Bao X., Hahn-Hägerdal B. Expression of different levels of enzymes from the Pichia stipitis XYL1 and XYL2 genes in Saccharomyces cerevisiae and its effects on product formation during xylose utilisation. Appl. Microbiol. Biotechnol. 48:1997;218-224.
10. Johansson B., Christensson C., Hobley T., Hahn-Hägerdal B. Xylulokinase overexpression in two strains of Saccharomyces cerevisiae also expressing xylose reductase and xylitol dehydrogenase and its effect on fermentation of xylose and lignocellulosic hydrolysate. Appl. Environ. Microbiol. 67:2001;4249-4255.
11. Bhosale S.H., Rao M.B., Deshpande V.V. Molecular and industrial aspects of glucose isomerase. Microbiol. Rev. 60:1996;280-300.
12. Hahn-Hägerdal B., Wahlbom C.F., Gárdonyi M., van Zyl W.H., Cordero Otero R.R., Jönsson L.J. Metabolic engineering of Saccharomyces cerevisiae for xylose utilisation. Adv. Biochem. Eng. Biotechnol. 73:2001;53-84.
13. Amore R., Wilhelm M., Hollenberg C.P. The fermentation of xylose - an analysis of the expression of Bacillus and Actinoplanes xylose isomerase genes in yeast. Appl. Microbiol. Biotechnol. 30:1989;351-357.
14. Sarthy A.V., McConaughy B.L., Lobo Z., Sundstrom J.A., Furlong C.E., Hall B.D. Expression of the Escherichia coli xylose isomerase gene in Saccharomyces cerevisiae. Appl. Environ. Microbiol. 53:1987;1996-2000.
15. Moes C.J., Pretorius I.S., van Zyl W.H. Cloning and expression of the Clostridium thermosulfurogenes D-xylose isomerase gene (xylA) in Saccharomyces cerevisiae. Biotechnol. Lett. 18:1996;269-274.
16. Walfridsson M., Bao X., Anderlund M., Lilius G., Bülow L., Hahn-Hägerdal B. Ethanolic fermentation of xylose with Saccharomyces cerevisiae harboring the Thermus thermophilus xylA gene which expresses an active xylose (glucose) isomerase. Appl. Environ. Microbiol. 62:1996;4648-4651.
17. Ho N.W.Y., Stevis P., Rosenfeld S., Huang J.J., Tsao G.T. Expression of the Escherichia coli isomerase gene by a yeast promoter. Biotechnol. Bioeng. Symp. 13:1983;245-250.
18. Hallborn J. Metabolic Engineering of Saccharomyces cerevisiae: Expression of Genes Involved in Pentose Metabolism. Ph.D. thesis, Lund University; 1995.
19. Kristo P., Saarelainen R., Fagerstrom R., Aho S., Korhola M. Protein purification, and cloning and characterization of the cDNA and gene for xylose isomerase of barley. Eur. J. Biochem. 237:1996;240-246.
20. Suomalainen I, Aho S, Saarelainen R, Kristo P. Plant xylose isomerase. WO 96/24667; 1996.
21. Drummond RJ, Bloch W, Matthews BW, Toy PL, Nicholson HH. Procaryotic xylose isomerase muteins and method to increase protein stability. WO89/01520; 1989.
22. Haldrup A., Petersen S.G., Okkels F.T. Positive selection: a plant selection principle based on xylose isomerase an enzyme used in the food industry. Plant Cell Reports. 18:1998;76-81.
23. Whitaker R.D., Cho Y., Cha J., Carrell H.L., Glusker J.P., Karplus P.A.et al. Probing the roles of active site residues in D-xylose isomerase. J. Biol. Chem. 270:1995;22895-22906.
24. Carrell H.L., Rubin B.H., Hurley T.J., Glusker J.P. X-ray crystal structure of D-xylose isomerase at 4-Å resolution. J. Biol. Chem. 259:1984;3230-3236.
25. Henrick K., Blow D.M., Carrell H.L., Glusker J.P. Comparison of backbone structures of glucose isomerase from Streptomyces and Arthrobacter. Protein Eng. 1:1987;467-469.
26. Carrell H.L., Glusker J.P., Burger V., Manfre F., Tritsch D., Biellmann J.F. X-ray analysis of D-xylose isomerase at 1.9 Å: native enzyme in complex with substrate and with a mechanism-designed inactivator. Proc. Natl. Acad. Sci. USA. 86:1989;4440-4444.
27. Whitlow M., Howard A.J., Finzel B.C., Poulos T.L., Winborne E., Gilliland G.L. A metal-mediated hydride shift mechanism for xylose isomerase based on the 1.6 Å Streptomyces rubiginosus structures with xylitol and D-xylose. Proteins. 9:1991;153-173.
28. Ausubel FM, Brent R, Kingston RE, Moore DD, Seidman JG, Smith JA, Struhl K. Current Protocols in Molecular Biology. New York: Wiley; 1997.
29. Gietz R.D., Sugino A. New yeast-Escherichia coli shuttle vectors constructed with in vitro mutagenized yeast genes lacking six-base pair restriction sites. Gene. 74:1988;527-534.
30. Alting-Mees M.A., Short J.M. pBluescript II: gene mapping vectors. Nucleic Acids Res. 17:1989;9494.
31. Brown A.J.P., Tuite M. Appendix II: yeast growth. Methods Microbiol. 26:1998;471-477.
32. Entian K.-D., Kötter P. Yeast mutant and plasmid collections. Methods Microbiol. 26:1998;431-449.
33. Schmitt M.E., Brown T.A., Trumpower B.L. A rapid and simple method for preparation of RNA from Saccharomyces cerevisiae. Nucleic. Acids Res. 18:1990;3091-3092.
34. Liu W., Saint D.A. A new quantitative method of real time reverse transcription polymerase chain reaction assay based on simulation of polymerase chain reaction kinetics. Anal. Biochem. 302:2002;52-59.
35. Callens M., Kersters-Hilderson H., van Opstal O., de Bruyne C.K. Catalytic properties of D-xylose isomerase from Streptomyces violaceoruber. Enzyme Microb. Technol. 8:1986;696-700.
36. Bernstein F.C., Koetzle T.F., Williams G.J., Meyer E.E. Jr., Brice M.D., Rodgers J.R.et al. The Protein Data Bank: a computer-based archival file for macromolecular structures. J. Mol. Biol. 112:1977;535-542.
37. Guex N., Peitsch M.C. SWISS-MODEL and the Swiss-PdbViewer: an environment for comparative protein modeling. Electrophoresis. 18:1997;2714-2723.
38. Mellor J., Dobson M.J., Roberts N.A., Tuite M.F., Emtage J.S., White S.et al. Efficient synthesis of enzymatically active calf chymosin in Saccharomyces cerevisiae. Gene. 24:1983;1-14.
39. Dombek K.M., Ingram L.O. Ethanol production during batch fermentation with Saccharomyces cerevisiae: changes in glycolytic enzymes and internal pH. Appl. Environ. Microbiol. 53:1987;1286-1291.
40. Rangarajan M., Asboth B., Hartley B.S. Stability of Arthrobacter D-xylose isomerase to denaturants and heat. Biochem. J. 285:1992;889-898.
41. Gaikwad S., Rao M., Deshpande V. Structure-function relationship of glucose/xylose isomerase from Streptomyces: evidence for the occurrence of inactive dimer. Enzyme Microb. Technol. 15:1993;155-157.
42. Chang C., Park B.C., Lee D.S., Suh S.W. Crystal structures of thermostable xylose isomerases from Thermus caldophilus and Thermus thermophilus: possible structural determinants of thermostability. J. Mol. Biol. 288:1999;623-634.
43. Lee D.H., Goldberg A.L. Selective inhibitors of the proteasome-dependent and vacuolar pathways of protein degradation in Saccharomyces cerevisiae. J. Biol. Chem. 271:1996;27280-27284.
44. Jones E.W. Tackling the protease problem in Saccharomyces cerevisiae. Methods Enzymol. 194:1991;428-453.
45. Lee D.H., Goldberg A.L. Proteasome inhibitors cause induction of heat shock proteins and trehalose which together confer thermotolerance in Saccharomyces cerevisiae. Mol. Cell Biol. 18:1998;30-38.
46. Shusta E.V., Raines R.T., Plückthun A., Wittrup K.D. Increasing the secretory capacity of Saccharomyces cerevisiae for production of single-chain antibody fragments. Nat. Biotechnol. 16:1998;773-777.
47. Lönn A., Gárdonyi M., van Zyl W.H., Hahn-Hägerdal B., Cordero Otero R.R. Cold adaptation of xylose isomerase from Thermus thermophilus through random PCR mutagenesis. Gene cloning and protein characterization. Eur. J. Biochem. 269:2002;157-163.