Contribution of a neopullulanase, a pullulanase, and an α-glucosidase to growth of Bacteroides thetaiotaomicron on starch

Journal of Bacteriology

Volumn 178, Issue 24, 1996, Pages 7173-7179

  D'Elia, John N ^a   Salyers, Abigail A ^a  

^a UNIVERSITY OF ILLINOIS AT URBANA CHAMPAIGN   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ALPHA GLUCOSIDASE; PULLULANASE; STARCH;

ARTICLE; BACTERIAL GROWTH; BACTEROIDES; CARBOHYDRATE METABOLISM; ENZYME ACTIVITY; GENE DISRUPTION; GENE SEQUENCE; MOLECULAR CLONING; NONHUMAN; PRIORITY JOURNAL;

BACTERIA (MICROORGANISMS); BACTEROIDES THETAIOTAOMICRON; NEGIBACTERIA;

EID: 0030479827     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/jb.178.24.7173-7179.1996     Document Type: Article

References (18)

1. Anderson, K., and A. A. Salyers. 1989. Biochemical evidence that starch breakdown by Bacteroides thetaiotaomicron involves outer membrane starch binding sites and periplasmic starch-degrading enzymes. J. Bacteriol. 171: 3192-3198.
2. Anderson, K., and A. A. Salyers. 1989. Genetic evidence that outer membrane binding of starch is required for starch utilization by Bacteroides thetaiotaomicron. J. Bacteriol. 171:3199-3204.
3. Cheng, Q., V. Hwa, and A. A. Salyers. 1992. A locus that contributes to colonization of the intestinal tract by Bacteroides thetaiotaomicron contains a single regulatory gene (chuR) that links two polysaccharide utilization pathways. J. Bacteriol. 174:7185-7193.
4. Devereux, J., P. Haeberli, and O. Smithies. 1984. A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res. 12:387-395.
5. Feldhaus, M. J., Q. Cheng, V. Hwa, and A. A. Salyers. 1991 Use of Escherichia coli β-glucuronidase (GUS) gene as a reporter gene for investigation of Bacteroides promoters. J. Bacteriol. 173:4540-4543.
6. Hanahan, D. 1983. Studies on transformation of Escherichia coli with plasmids. J. Mol. Biol. 166:557-580.
7. Janse, B. J. H., A. J. C. Steyne, and I. S. Pretorius. 1993. Regional sequence homologies in starch-degrading enzymes. Curr. Genet. 24:400-407.
8. Kotarski, S. F., and A. A. Salyers. 1984. Isolation and characterization of outer membranes from Bacteroides thetaiotaomicron grown on different carbohydrates. J. Bacteriol. 158:102-109.
9. Lederberg, E. M., and S. M. Cohen. 1974. Transformation of Salmonella typhimurium by plasmid deoxyribonucleic acid. J. Bacteriol. 119:1072-1074.
10. Maniatis, T., E. F. Fritsch, and J. Sambrook. 1982. Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
11. Reeves, A., J. D'Elia, J. Frias, and A. A. Salyers. 1996. A Bacteroides thetaiotaomicron outer membrane protein that is essential for utilization of maltooligosaccharides and starch. J. Bacteriol. 178:823-830.
12. Saito, H., and K. I. Miura. 1963. Preparation of transforming deoxyribonucleic acid by phenol treatment. Biochim. Biophys. Acta 72:619-629.
13. Smith, K., and A. A. Salyers. 1989. Characterization of a cell-associated Bacteroides pullulanase and determination of its contribution to pullulan utilization. J. Bacteriol. 171:2116-2123.
14. Smith, K., and A. A. Salyers. 1991. Purification and characterization of enzymes involved in starch utilization by Bacteroides thetaiotaomicron. J. Bacteriol. 173:2962-2968.
15. Stevens, A. M., N. B. Shoemaker, and A. A. Salyers. 1990. The region of Bacteroides conjugal chromosomal tetracycline resistance elements which is responsible for production of plasmidlike form from unlinked chromosomal DNA might also be involved in transfer of the element. J. Bacteriol. 172: 4271-4279.
16. Studier, F. W., and B. A. Moffet. 1986. Use of T7 RNA polymerase to direct selective high-level expression of cloned genes. J. Mol. Biol. 189:113-130.
17. Svensson, B. 1994. Protein engineering in the α-amylase family: catalytic mechanism, substrate specificity and stability. Plant Mol. Biol. 25:141-157.
18. Tancula, E., M. J. Feldhaus, L. A. Bedzyk, and A. A. Salyers. 1992. Location and characterization of genes involved in binding of starch to the surface of Bacteroides thetaiotaomicron. J. Bacteriol. 174:5609-5610.