Improvements to the Kunkel mutagenesis protocol for constructing primary and secondary phage-display libraries

Methods

Volumn 58, Issue 1, 2012, Pages 10-17

  Huang, Renhua ^a   Fang, Pete ^a   Kay, Brian K ^a  

^a UNIVERSITY OF ILLINOIS AT CHICAGO   (United States)

Author keywords

Affinity selection; Asymmetric PCR; Diversity; Error prone PCR; FN3 monobody; Kunkel mutagenesis; Library construction; M13 bacteriophage; Mutation rate; Mutazyme II

Indexed keywords

FIBRONECTIN; PROTEIN VARIANT; SINGLE STRANDED DNA;

ARTICLE; CLONE; DNA MODIFICATION; HUMAN; HUMAN CELL; KUNKEL MUTAGENESIS; MUTAGENESIS; NONHUMAN; PHAGE DISPLAY; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL;

BACTERIOPHAGE M13; CELL LINE; CELL SURFACE DISPLAY TECHNIQUES; CLONING, MOLECULAR; DNA CLEAVAGE; DNA RESTRICTION ENZYMES; DNA, SINGLE-STRANDED; ESCHERICHIA COLI; FIBRONECTINS; GENETIC VECTORS; HUMANS; MUTAGENESIS, SITE-DIRECTED; P21-ACTIVATED KINASES; PEPTIDE LIBRARY; PLASMIDS; POLYMERASE CHAIN REACTION; PROTEIN BINDING; SEQUENCE ANALYSIS, DNA; TRANSFORMATION, BACTERIAL;

EID: 84868371381     PISSN: 10462023     EISSN: 10959130     Source Type: Journal    
DOI: 10.1016/j.ymeth.2012.08.008     Document Type: Article

References (32)

1. Wells J.A., Estell D.A. Trends Biochem. Sci. 1988, 13:291-297.
2. Vandeyar M.A., Weiner M.P., Hutton C.J., Batt C.A. Gene 1988, 65:129-133.
3. Sugimoto M., Esaki N., Tanaka H., Soda K. Anal. Biochem. 1989, 179:309-311.
4. Kunkel T.A. Proc. Natl. Acad. Sci. USA 1985, 82:488-492.
5. Kunkel T.A., Roberts J.D., Zakour R.A. Methods Enzymol. 1987, 154:367-382.
6. Scholle M.D., Kehoe J.W., Kay B.K. Comb. Chem. High Throughput Screen. 2005, 8:545-551.
7. Tonikian R., Zhang Y., Boone C., Sidhu S.S. Nat. Protoc. 2007, 2:1368-1386.
8. Wojcik J., Hantschel O., Grebien F., Kaupe I., Bennett K.L., Barkinge J., Jones R.B., Koide A., Superti-Furga G., Koide S. Nat. Struct. Mol. Biol. 2010, 17:519-527.
9. Fellouse F.A., Esaki K., Birtalan S., Raptis D., Cancasci V.J., Koide A., Jhurani P., Vasser M., Wiesmann C., Kossiakoff A.A., Koide S., Sidhu S.S. J. Mol. Biol. 2007, 373:924-940.
10. Huang R., Fang P., Kay B.K. N. Biotechnol. 2012, 29:526-533.
11. Ling M.M. Comb. Chem. High Throughput Screen. 2003, 6:421-432.
12. Menendez A., Scott J.K. Anal. Biochem. 2005, 336:145-157.
13. Brammer L.A., Bolduc B., Kass J.L., Felice K.M., Noren C.J., Hall M.F. Anal. Biochem. 2008, 373:88-98.
14. Fagete S., Ravn U., Gueneau F., Magistrelli G., Kosco-Vilbois M.H., Fischer N. MAbs 2009, 1:288-296.
15. Yang W.P., Green K., Pinz-Sweeney S., Briones A.T., Burton D.R., Barbas C.F. J. Mol. Biol. 1995, 254:392-403.
16. Groves M., Lane S., Douthwaite J., Lowne D., Rees D.G., Edwards B., Jackson R.H. J. Immunol. Methods 2006, 313:129-139.
17. Gram H., Marconi L.A., Barbas C.F., Collet T.A., Lerner R.A., Kang A.S. Proc. Natl. Acad. Sci. USA 1992, 89:3576-3580.
18. Daugherty P.S., Chen G., Iverson B.L., Georgiou G. Proc. Natl. Acad. Sci. USA 2000, 97:2029-2034.
19. Koide A., Bailey C.W., Huang X., Koide S. J. Mol. Biol. 1998, 284:1141-1151.
20. Koide S., Koide A., Lipovsek D. Methods Enzymol. 2012, 503:135-156.
21. Karatan E., Merguerian M., Han Z., Scholle M.D., Koide S., Kay B.K. Chem. Biol. 2004, 11:835-844.
22. Pershad K., Sullivan M.A., Kay B.K. Anal. Biochem. 2011, 412:210-216.
23. Haidaris C.G., Malone J., Sherrill L.A., Bliss J.M., Gaspari A.A., Insel R.A., Sullivan M.A. J. Immunol. Methods 2001, 257:185-202.
24. Lindahl T., Ljungquist S., Siegert W., Nyberg B., Sperens B. J. Biol. Chem. 1977, 252:3286-3294.
25. Manser E., Leung T., Salihuddin H., Zhao Z.S., Lim L. Nature 1994, 367:40-46.
26. Parrini M.C., Lei M., Harrison S.C., Mayer B.J. Mol. Cell. 2002, 9:73-83.
27. Tessier D.C., Thomas D.Y. Methods Mol. Biol. 1996, 57:229-237.
28. Cadwell R.C., Joyce G.F. PCR Methods Appl. 1994, 3:S136-140.
29. Ellis E., Delbrück M. J. Gen. Physiol. 1938, 22:365-384.
30. Sagher D., Strauss B. Biochemistry 1983, 22:4518-4526.
31. Rasila T.S., Pajunen M.I., Savilahti H. Anal. Biochem. 2009, 388:71-80.
32. Pai J.C., Entzminger K.C., Maynard J.A. Anal. Biochem. 2012, 421:640-648.