Pseudomonas aeruginosa directly shunts β-oxidation degradation intermediates into de novo fatty acid biosynthesis

Journal of Bacteriology

Volumn 194, Issue 19, 2012, Pages 5185-5196

  Yuan, Yanqiu ^a   Leeds, Jennifer A ^a   Meredith, Timothy C ^a  

^a NOVARTIS INSTITUTES FOR BIOMEDICAL RESEARCH   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ACYL CARRIER PROTEIN; ACYL COENZYME A; BETA KETO DECANOYL ACYL CARRIER PROTEIN; BETA KETOACYL SYNTHASE FABY; DECANOIC ACID; LIPOPOLYSACCHARIDE; SATURATED FATTY ACID; SYNTHETASE; THIOESTER; UNCLASSIFIED DRUG; UNSATURATED FATTY ACID;

ARTICLE; CONTROLLED STUDY; CULTURE MEDIUM; ENZYME SPECIFICITY; ESCHERICHIA COLI; FATTY ACID OXIDATION; FATTY ACID SYNTHESIS; GENE EXPRESSION; IN VITRO STUDY; LIPID DEGRADATION; NONHUMAN; OPEN READING FRAME; PRIORITY JOURNAL; PSEUDOMONAS AERUGINOSA;

3-OXOACYL-(ACYL-CARRIER-PROTEIN) SYNTHASE; CLONING, MOLECULAR; ESCHERICHIA COLI; FATTY ACIDS; GENE EXPRESSION REGULATION, BACTERIAL; GENETIC COMPLEMENTATION TEST; MOLECULAR STRUCTURE; OXIDATION-REDUCTION; PSEUDOMONAS AERUGINOSA;

ESCHERICHIA COLI; PSEUDOMONAS AERUGINOSA;

EID: 84868306426     PISSN: 00219193     EISSN: 10985530     Source Type: Journal    
DOI: 10.1128/JB.00860-12     Document Type: Article

References (60)

1. Bernhard W, et al. 1997. Lung surfactant in a cystic fibrosis animal model: increased alveolar phospholipid pool size without altered composition and surface tension function in cftrm1HGU/m1HGU mice. Thorax 52: 723-730.
2. Black PN, DiRusso CC. 1994. Molecular and biochemical analyses of fatty acid transport, metabolism, and gene regulation in Escherichia coli. Biochim. Biophys. Acta 1210:123-145.
3. Breidenstein EB, Fuente-Nunez C, Hancock RE. 2011. Pseudomonas aeruginosa: all roads lead to resistance. Trends Microbiol. 19:419-426.
4. Brinster S, et al. 2009. Type II fatty acid synthesis is not a suitable antibiotic target for Gram-positive pathogens. Nature 458:83-86.
5. Brown AK, et al. 2005. Probing the mechanism of the Mycobacterium tuberculosis beta-ketoacyl-acyl carrier protein synthase III mtFabH: factors influencing catalysis and substrate specificity. J. Biol. Chem. 280: 32539-32547.
6. Brugha RE, Davies JC. 2011. Pseudomonas aeruginosa in cystic fibrosis: pathogenesis and new treatments. Br. J. Hosp. Med. (Lond.) 72:614-619.
7. Byers DM. 1989. Elongation of exogenous fatty acids by the bioluminescent bacterium Vibrio harveyi. J. Bacteriol. 171:59-64.
8. Choi KH, Kremer L, Besra GS, Rock CO. 2000. Identification and substrate specificity of beta-ketoacyl (acyl carrier protein) synthase III (mtFabH) from Mycobacterium tuberculosis. J. Biol. Chem. 275:28201-28207.
9. Choi KH, Schweizer HP. 2005. An improved method for rapid generation of unmarked Pseudomonas aeruginosa deletion mutants. BMC Microbiol. 5:30. doi:10.1186/1471-2180-5-30.
10. Clark DP, Cronan JE. 1996. Two-carbon compounds and fatty acids as carbon sources, p 343-358. In Neidhardt FC, et al (ed), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed, vol 1. American Society for Microbiology, Washington, DC.
11. Cronan JE, Lin S. 2011. Synthesis of the alpha, omega-dicarboxylic acid precursor of biotin by the canonical fatty acid biosynthetic pathway. Curr. Opin. Chem. Biol. 15:407-413.
12. Cronan JE, Rock CO. 1996. Biosynthesis of membrane lipids, p 612-636. In Neidhardt FC, et al (ed), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed, vol 1. American Society for Microbiology, Washington, DC.
13. Datsenko KA, Wanner BL. 2000. One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc. Natl. Acad. Sci. U. S. A. 97:6640-6645.
14. Davies DG, Marques CN. 2009. A fatty acid messenger is responsible for inducing dispersion in microbial biofilms. J. Bacteriol. 191:1393-1403.
15. Driscoll JA, Brody SL, Kollef MH. 2007. The epidemiology, pathogenesis and treatment of Pseudomonas aeruginosa infections. Drugs 67:351-368.
16. Figurski DH, Helinski DR. 1979. Replication of an origin-containing derivative of plasmid RK2 dependent on a plasmid function provided in trans. Proc. Natl. Acad. Sci. U. S. A. 76:1648-1652.
17. Fujita Y, Matsuoka H, Hirooka K. 2007. Regulation of fatty acid metabolism in bacteria. Mol. Microbiol. 66:829-839.
18. Gajiwala KS, et al. 2009. Crystal structures of bacterial FabH suggest a molecular basis for the substrate specificity of the enzyme. FEBS Lett. 583:2939-2946.
19. Galabert C, Jacquot J, Zahm JM, Puchelle E. 1987. Relationships between the lipid content and the rheological properties of airway secretions in cystic fibrosis. Clin. Chim. Acta 164:139-149.
20. Geller DE. 2009. Aerosol antibiotics in cystic fibrosis. Respir. Care 54: 658-670.
21. Giles DK, Hankins JV, Guan Z, Trent MS. 2011. Remodelling of the Vibrio cholerae membrane by incorporation of exogenous fatty acids from host and aquatic environments. Mol. Microbiol. 79:716-728.
22. Hannauer M, et al. 2012. Biosynthesis of the pyoverdine siderophore of Pseudomonas aeruginosa involves precursors with a myristic or a myristoleic acid chain. FEBS Lett. 586:96-101.
23. Heath RJ, Rock CO. 1996. Inhibition of beta-ketoacyl-acyl carrier protein synthase III (FabH) by acyl-acyl carrier protein in Escherichia coli. J. Biol. Chem. 271:10996-11000.
24. Hidron AI, et al. 2008. NHSN annual update: antimicrobial-resistant pathogens associated with healthcare-associated infections: annual summary of data reported to the National Healthcare Safety Network at the Centers for Disease Control and Prevention, 2006-2007. Infect. Control Hosp. Epidemiol. 29:996-1011.
25. Hoang TT, Karkhoff-Schweizer RR, Kutchma AJ, Schweizer HP. 1998. A broad-host-range Flp-FRT recombination system for site-specific excision of chromosomally-located DNA sequences: application for isolation of unmarked Pseudomonas aeruginosa mutants. Gene 212:77-86.
26. Hoang TT, Kutchma AJ, Becher A, Schweizer HP. 2000. Integrationproficient plasmids for Pseudomonas aeruginosa: site-specific integration and use for engineering of reporter and expression strains. Plasmid 43:59-72.
27. Ingram LO, Chevalier LS, Gabba EJ, Ley KD, Winters K. 1977. Propionate-induced synthesis of odd-chain-length fatty acids by Escherichia coli. J. Bacteriol. 131:1023-1025.
28. Jiang Y, Chan CH, Cronan JE. 2006. The soluble acyl-acyl carrier protein synthetase of Vibrio harveyi B392 is a member of the medium chain acyl-CoA synthetase family. Biochemistry 45:10008-10019.
29. Jiang Y, Morgan-Kiss RM, Campbell JW, Chan CH, Cronan JE. 2010. Expression of Vibrio harveyi acyl-ACP synthetase allows efficient entry of exogenous fatty acids into the Escherichia coli fatty acid and lipid A synthetic pathways. Biochemistry 49:718-726.
30. Jimenez PN, et al. 2012. The multiple signaling systems regulating virulence in Pseudomonas aeruginosa. Microbiol. Mol. Biol. Rev. 76:46-65.
31. Kang Y, Nguyen DT, Son MS, Hoang TT. 2008. The Pseudomonas aeruginosa PsrA responds to long-chain fatty acid signals to regulate the fadBA5 beta-oxidation operon. Microbiology 154:1584-1598.
32. Kang Y, Zarzycki-Siek J, Walton CB, Norris MH, Hoang TT. 2010. Multiple FadD acyl-CoA synthetases contribute to differential fatty acid degradation and virulence in Pseudomonas aeruginosa. PLoS One 5:e13557. doi:10.1371/journal.pone.0013557.
33. Kass LR, Brock DJ, Bloch K. 1967. Beta-hydroxydecanoyl thioester dehydrase. I. Purification and properties. J. Biol. Chem. 242:4418-4431.
34. Kerr KG, Snelling AM. 2009. Pseudomonas aeruginosa: a formidable and ever-present adversary. J. Hosp. Infect. 73:338-344.
35. King JD, Kocincova D, Westman EL, Lam JS. 2009. Lipopolysaccharide biosynthesis in Pseudomonas aeruginosa. Innate Immun. 15:261-312.
36. Klein K, Steinberg R, Fiethen B, Overath P. 1971. Fatty acid degradation in Escherichia coli. An inducible system for the uptake of fatty acids and further characterization of old mutants. Eur. J. Biochem. 19:442-450.
37. Lin S, Hanson RE, Cronan JE. 2010. Biotin synthesis begins by hijacking the fatty acid synthetic pathway. Nat. Chem. Biol. 6:682-688.
38. Lister PD, Wolter DJ, Hanson ND. 2009. Antibacterial-resistant Pseudomonas aeruginosa: clinical impact and complex regulation of chromosomally encoded resistance mechanisms. Clin. Microbiol. Rev. 22:582-610.
39. Moore NM, Flaws ML. 2011. Epidemiology and pathogenesis of Pseudomonas aeruginosa infections. Clin. Lab Sci. 24:43-46.
40. Okuda S, Tokuda H. 2011. Lipoprotein sorting in bacteria. Annu. Rev. Microbiol. 65:239-259.
41. Parsons JB, Rock CO. 2011. Is bacterial fatty acid synthesis a valid target for antibacterial drug discovery? Curr. Opin. Microbiol. 14:544-549.
42. Patton GM, Lowenstein JM. 1979. Measurements of fatty acid synthesis by incorporation of deuterium from deuterated water. Biochemistry 18: 3186-3188.
43. Raetz CR, Whitfield C. 2002. Lipopolysaccharide endotoxins. Annu. Rev. Biochem. 71:635-700.
44. Remans K, Vercammen K, Bodilis J, Cornelis P. 2010. Genome-wideanalysis and literature-based survey of lipoproteins in Pseudomonas aeruginosa. Microbiology 156:2597-2607.
45. Rietsch A, Mekalanos JJ. 2006. Metabolic regulation of type III secretion gene expression in Pseudomonas aeruginosa. Mol. Microbiol. 59:807-820.
46. Rock CO, Cronan JE. 1981. Acyl carrier protein from Escherichia coli. Methods Enzymol. 7(Part C):341-351.
47. Silbert DF, Ruch F, Vagelos PR. 1968. Fatty acid replacements in a fatty acid auxotroph of Escherichia coli. J. Bacteriol. 95:1658-1665.
48. Son MS, Matthews WJ, Jr, Kang Y, Nguyen DT, Hoang TT. 2007. In vivo evidence of Pseudomonas aeruginosa nutrient acquisition and pathogenesis in the lungs of cystic fibrosis patients. Infect. Immun. 75:5313-5324.
49. Spalding MD, Prigge ST. 2010. Lipoic acid metabolism in microbial pathogens. Microbiol. Mol. Biol. Rev. 74:200-228.
50. Stanier RY, Palleroni NJ, Doudoroff M. 1966. The aerobic pseudomonads: a taxonomic study. J. Gen. Microbiol. 43:159-271.
51. Stover CK, et al. 2000. Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen. Nature 406:959-964.
52. Tsay JT, Oh W, Larson TJ, Jackowski S, Rock CO. 1992. Isolation and characterization of the beta-ketoacyl-acyl carrier protein synthase III gene (fabH) from Escherichia coli K-12. J. Biol. Chem. 267:6807-6814.
53. Weeks G, Shapiro M, Burns RO, Wakil SJ. 1969. Control of fatty acid metabolism. I. Induction of the enzymes of fatty acid oxidation in Escherichia coli. J. Bacteriol. 97:827-836.
54. Williams P, Camara M. 2009. Quorum sensing and environmental adaptation in Pseudomonas aeruginosa: a tale of regulatory networks and multifunctional signal molecules. Curr. Opin. Microbiol. 12:182-191.
55. Wu DC, Chan WW, Metelitsa AI, Fiorillo L, Lin AN. 2011. Pseudomonas skin infection: clinical features, epidemiology, and management. Am. J. Clin. Dermatol. 12:157-169.
56. Yuan Y, Sachdeva M, Leeds JA, Meredith TC. 2012. Fatty acid biosynthesis in Pseudomonas aeruginosa is initiated by the FabY class of _-ketoacyl acyl carrier protein synthases. J. Bacteriol. 194:5171-5184.
57. Zhang YM, Frank MW, Zhu K, Mayasundari A, Rock CO. 2008. PqsD is responsible for the synthesis of 2,4-dihydroxyquinoline, an extracellular metabolite produced by Pseudomonas aeruginosa. J. Biol. Chem. 283: 28788-28794.
58. Zhang YM, Rock CO. 2008. Membrane lipid homeostasis in bacteria. Nat. Rev. Microbiol. 6:222-233.
59. Zhang YM, Rock CO. 2009. Transcriptional regulation in bacterial membrane lipid synthesis. J. Lipid Res. 50(Suppl):S115-S119.
60. Zhu K, Choi KH, Schweizer HP, Rock CO, Zhang YM. 2006. Two aerobic pathways for the formation of unsaturated fatty acids in Pseudomonas aeruginosa. Mol. Microbiol. 60:260-273.