Toward a reference method for leukocyte differential counts in blood: Comparison of three flow cytometric candidate methods

Cytometry Part A

Volumn 81 A, Issue 11, 2012, Pages 973-982

  Roussel, Mikael ^a   Davis, Bruce H ^b   Fest, Thierry ^a,c,d   Wood, Brent L ^e  

^a RENNES UNIVERSITY HOSPITAL   (France)

^b Trillium Diagnostics   (United States)

^c INSERM U917   (France)

^d UNIVERSITÉ DE RENNES 1   (France)

^e UNIVERSITY OF WASHINGTON   (United States)

Author keywords

Complete blood count; Flow cytometry; Laboratory instrumentation; Leukocyte differential; Reference method

Indexed keywords

EID: 84867909141     PISSN: 15524922     EISSN: 15524930     Source Type: Journal    
DOI: 10.1002/cyto.a.22092     Document Type: Article

References (20)

1. Barnes PW, McFadden SL, Machin SJ, Simson E. The international consensus group for hematology review: Suggested criteria for action following automated CBC and WBC differential analysis. Lab Hematol 2005; 11: 83-90.
2. Novis DA, Walsh M, Wilkinson D, St Louis M, Ben-Ezra J. Laboratory productivity and the rate of manual peripheral blood smear review: A College of American Pathologists Q-Probes study of 95, 141 complete blood count determinations performed in 263 institutions. Arch Pathol Lab Med 2006; 130: 596-601.
3. Briggs C, Longair I, Slavik M, Thwaite K, Mills R, Thavaraja V, Foster A, Romanin D, Machin SJ. Can automated blood film analysis replace the manual differential? An evaluation of the CellaVision DM96 automated image analysis system. Int J Lab Hematol 2009; 31: 48-60.
4. Bain BJ. Diagnosis from the blood smear. N Engl J Med 2005; 353: 498-507.
5. Clinical and Laboratory Standards Institute. Reference leukocyte (WBC) differential count (proportional) and evaluation of instrument methods; Approved standard. 2007; 27.
6. Rumke CL. Imprecision of ratio-derived differential leukocyte counts. Blood Cells 1985; 11: 311-4, 315.
7. Roussel M, Benard C, Ly-Sunnaram B, Fest T. Refining the white blood cell differential: The first flow cytometry routine application. Cytometry A 2010; 77A: 552-563.
8. Cherian S, Levin G, Lo WY, Mauck M, Kuhn D, Lee C, Wood BL. Evaluation of an 8-color flow cytometric reference method for white blood cell differential enumeration. Cytometry B Clin Cytom 2010; 78B: 319-328.
9. Björnsson S, Wahlström S, Norström E, Bernevi I, O'neill U, Johansson E, Runström H, Simonsson P. Total nucleated cell differential for blood and bone marrow using a single tube in a five-color flow cytometer. Cytometry B Clin Cytom 2008; 74B: 91-103.
10. Faucher J-L, Lacronique-Gazaille C, Frébet E, Trimoreau F, Donnard M, Bordessoule D, Lacombe F, Feuillard J. "6 markers/5 colors" extended white blood cell differential by flow cytometry. Cytometry A 2007; 71A(11): 934-944.
11. Hubl W, Tlustos L, Erath A, Andert S, Bayer PM. Proposed reference method for peripheral-blood monocyte counting using fluorescence-labeled monoclonal antibodies. Cytometry 1996; 26: 69-74.
12. Fujimoto H, Sakata T, Hamaguchi Y, Shiga S, Tohyama K, Ichiyama S, Wang FS, Houwen B. Flow cytometric method for enumeration and classification of reactive immature granulocyte populations. Cytometry 2000; 42: 371-378.
13. Finn LS, Hall J, Xu M, Rutledge JC. Flow cytometric validation of automated differentials in pediatric patients. Lab Hematol 2004; 10: 112-118.
14. Della Bella S, Giannelli S, Taddeo A, Presicce P, Villa ML. Application of six-color flow cytometry for the assessment of dendritic cell responses in whole blood assays. J Immunol Methods 2008; 339: 153-164.
15. Giannelli S, Taddeo A, Presicce P, Villa ML, Della Bella S. A six-color flow cytometric assay for the analysis of peripheral blood dendritic cells. Cytometry B Clin Cytom 2008; 74B: 349-355.
16. Van De Geijn G-J, Van Rees V, Van Pul-Bom N, Birnie E, Janssen H, Pegels H, Beunis M, Njo T. Leukoflow: Multiparameter extended white blood cell differentiation for routine analysis by flow cytometry. Cytometry A 2011; 79A: 694-706.
17. Cid J, Aguinaco R, Sanchez R, Garcia-Pardo G, Llorente A. Neutrophil CD64 expression as marker of bacterial infection: A systematic review and meta-analysis. J Infect 2011; 60: 313-319.
18. Ouk C, Jayat-Vignoles C, Donnard M, Feuillard J. Both CD62 and CD162 antibodies prevent formation of CD36-dependent platelets, rosettes, and artefactual pseudoexpression of platelet markers on white blood cells: A study with ImageStream(R). Cytometry A 2011; 79A: 477-484.
19. Riccardi F, Della Porta MG, Rovati B, Casazza A, Radolovich D, De Amici M, Danova M, Langer M. Flow cytometric analysis of peripheral blood dendritic cells in patients with severe sepsis. Cytometry B Clin Cytom 2011; 80B: 14-21.
20. Saenz JJ, Izura JJ, Manrique A, Sala F, Gaminde I. Early prognosis in severe sepsis via analyzing the monocyte immunophenotype. Intensive Care Med 2001; 27: 970-977.