Total nucleated cell differential for blood and bone marrow using a single tube in a five-color flow cytometer

Cytometry Part B - Clinical Cytometry

Volumn 74, Issue 2, 2008, Pages 91-103

  Björnsson, Sven ^a   Wahlström, Saga ^a   Norström, Eva ^a   Bernevi, Ingela ^a   O'Neill, Ulla ^a   Johansson, Eva ^a   Runström, Håkan ^a   Simonsson, Per ^a  

^a LUND UNIVERSITY HOSPITAL   (Sweden)

Author keywords

Bone marrow; Cell cycle; DRAQ5; Leukocyte differential; Nuclear stain; Peripheral blood; Ploidy; Total nucleated count

Indexed keywords

DNA;

ARTICLE; BASOPHIL; BLAST CELL; BLOOD CELL COUNT; BLOOD CYTOLOGY; BLOOD SAMPLING; BONE MARROW CELL; CELL MATURATION; CELL NUCLEUS; CLASSIFICATION; CORRELATION COEFFICIENT; DILUTION; ERYTHROBLAST; FLOW CYTOMETER; FLOW CYTOMETRY; HUMAN; HUMAN CELL; IMMUNOPHENOTYPING; LEUKOCYTE COUNT; LYMPHOCYTE; LYMPHOCYTE COUNT; MEDICAL INSTRUMENTATION; MEGAKARYOCYTE; MICROSCOPY; MONOCYTE; NATURAL KILLER CELL; NEUTROPHIL; PLASMA CELL; PRIORITY JOURNAL; STAINING; THROMBOCYTE; THROMBOCYTE COUNT;

BLOOD CELL COUNT; BONE MARROW CELLS; CELL NUCLEUS; FLOW CYTOMETRY; HEMATOLOGIC DISEASES; HUMANS; IMMUNOPHENOTYPING; LEUKOCYTE COUNT; MICROSCOPY; PLATELET COUNT; REPRODUCIBILITY OF RESULTS;

EID: 42549115147     PISSN: 15524949     EISSN: 15524957     Source Type: Journal    
DOI: 10.1002/cyto.b.20382     Document Type: Article

References (33)

1. Houwen B. The differential count. Lab Hematol 2001;7:89-100.
2. Rümke CL. Imprecision of ratio-derived differential leukocyte counts. Blood Cells 1985;11:311-315.
3. Greve B, Beller C, Cassens U, Sibrowski W, Severin E, Gohde W. High-grade loss of leukocytes and hematopoietic progenitor cells caused by erythrocyte-lysing procedures for flow cytometric analyses. J Hematother Stem Cell Res 2003;12:321-330.
4. Terstappen LW, Loken MR. Five-dimensional flow cytometry as a new approach for blood and bone marrow differentials. Cytometry 1988;9:548-556.
5. Terstappen LW, Johnson D, Mickaels RA, Chen J, Olds G, Hawkins JT, Loken MR, Levin J. Multidimensional flow cytometric blood cell differentiation without erythrocyte lysis. Blood Cells 1991;17:585-602.
6. Terstappen LW, Levin J. Bone marrow cell differential counts obtained by multidimensional flow cytometry. Blood Cells 1992;18:311-330.
7. Hübl W, Wolfbauer G, Andert S, Thum G, Streicher J, Hubner C, Lapin A, Bayer PM. Toward a new reference method for the leukocyte five-part differential. Cytometry 1997;30:72-84.
8. Tsuji T, Sakata T, Hamaguchi Y, Wang F, Houwen B. New rapid flow cytometric method for the enumeration of nucleated red blood cells. Cytometry 1999;37:291-301.
9. Schlenke P, Frohn C, Kluter H, Saballus M, Hammers HJ, Zajac SR, Kirchner H. Evaluation of a flow cytometric method for simultaneous leukocyte phenotyping and quantification by fluorescent microspheres. Cytometry 1998;33:310-317.
10. Swolin B, Simonsson P, Backman S, Löfquist I, Bredin I, Johnsson M. Differential counting of blood leukocytes using automated microscopy and a decision support system based on artificial neural networks - Evaluation of DiffMaster TM Octavia. Clin Lab Haematol 2003;25:139-147.
11. Smith PJ, Blunt N, Wiltshire M, Hoy T, Teesdale-Spittle P, Craven MR, Watson JV, Amos WB, Errington RJ, Patterson LH. Characteristics of a novel deep red/infrared fluorescent cell-permeant DNA probe, DRAQ5, in intact human cells analyzed by flow cytometry, confocal and multiphoton microscopy. Cytometry 2000;40:280-291.
12. Njoh KL, Patterson LH, Zloh M, Wiltshire M, Fisher J, Chappell S, Ameer-Beg S, Bai Y, Matthews D, Errington RJ, Smith PJ. Spectral analysis of the DNA targeting bisalkylaminoanthraquinone DRAQ5 in intact living cells. Cytometry A 2006;69A:805-814.
13. Terstappen LW, Meiners H, Loken MR. A rapid sample preparation technique for flow cytometric analysis of immunofluorescence allowing absolute enumeration of cell subpopulations. J Immunol Methods 1989;123:103-112.
14. Vuorte J, Jansson SE, Repo H. Evaluation of red blood cell lysing solutions in the study of neutrophil oxidative burst by the DCFH assay. Cytometry 2001;43:290-296.
15. Greve B, Beller C, Cassens U, Sibrowski W, Göhde W. The impact of erythrocyte lysing procedures on the recovery of hematopoietic progenitor cells in flow cytometric analysis. Stem Cells 2006;24:793-799.
16. Harrison P, Ault KA, Chapman S, Charie L, Davis B, Fujimoto K, Houwen B, Kunicka J, Lacombe F, Machin S, Raynor R, van Hove L, van Assendelft OW;International Society of Laboratory Hematology Task Force for the Reference Platelet Count. An interlaboratory study of a candidate reference method for platelet counting. Am J Clin Pathol 2001;115:448-459.
17. van Schravendijk MR, Handunnetti SM, Barnwell JW, Howard RJ. Normal human erythrocytes express CD36, an adhesion molecule of monocytes, platelets, and endothelial cells. Blood 1992;80:2105-2114.
18. van Pampus EC, Denkers IA, van Geel BJ, Huijgens PC, Zevenbergen A, Ossenkoppele GJ, Langenhuijsen MM. Expression of adhesion antigens of human bone marrow megakaryocytes, circulating megakaryocytes and blood platelets. Eur J Haematol 1992;49:122-127.
19. Ducrest S, Meier F, Tschopp C, Pavlovic R, Dahinden CA. Flowcytometric analysis of basophil counts in human blood and inaccuracy of hematology analyzers. Allergy 2005;60:1446-1450.
20. Platz IJ, Binder M, Marxer A, Lischka G, Valent P, Buhring HJ. Hymenoptera-venom-induced upregulation of the basophil activation marker ecto-nucleotide pyrophosphatase/phosphodiesterase 3 in sensitized individuals. Int Arch Allergy Immunol 2001;126:335-342.
21. Ghannadan M, Hauswirth AW, Schernthaner GH, Muller MR, Klepetko W, Schatzl G, Sperr WR, Buhring HJ, Valent P. Detection of novel CD antigens on the surface of human mast cells and basophils. Int Arch Allergy Immunol 2002;127:299-307.
22. Boumiza R, Monneret G, Forissier MF, Savoye J, Gutowski MC, Powell WS, Bienvenu J. Marked improvement of the basophil activation test by detecting CD203c instead of CD63. Clin Exp Allergy 2003;33:259-265.
23. Terstappen LWMM, Z Hollander, Meiners H, Loken MR. Quantitative comparison of myeloid antigens on five lineages of mature peripheral blood cells. J Leukocyte Biol 1990;48:138-148.
24. Uguccioni M, Mackay CR, Ochensberger B, Loetscher P, Rhis S, LaRosa GJ, Rao P, Ponath PD, Baggiolini M, Dahinden CA. High expression of the chemokine receptor CCR3 in human blood basophils. Role in activation by eotaxin, MCP-4, and other chemokines. J Clin Invest 1997;100:1137-1143.
25. Bohm E, Sturm GJ, Weiglhofer I, Sandig H, Shichijo M, McNamee A, Pease JE, Kollroser M, Peskar BA, Heinemann A. 11-Dehydro-thromboxane B2, a stable thromboxane metabolite, is a full agonist of chemoattractant receptor-homologous molecule expressed on TH2 cells (CRTH2) in human eosinophils and basophils. J Biol Chem 2004;279:7663-7670.
26. Boumiza R, Debard AL, Monneret G. The basophil activation test by flow cytometry: recent developments in clinical studies, standardization and emerging perspectives. Clin Mol Allergy 2005;3:9.
27. Terstappen LW, Safford M, Loken MR. Flow cytometric analysis of human bone marrow. III. Neutrophil maturation. Leukemia 1990;4:657-663.
28. Fujimoto H, Sakata T, Hamaguchi Y, Shiga S, Tohyama K, Ichiyama S, Wang FS, Houwen B. Flow cytometric method for enumeration and classification of reactive immature granulocyte populations. Cytometry 2000;42:371-378.
29. Elghetany MT, Ge Y, Patel J, Martinez J, Uhrova H. Flow cytometric study of neutrophilic granulopoiesis in normal bone marrow using an expanded panel of antibodies: Correlation with morphologic assessments. J Clin Lab Anal 2004;18:36-41.
30. Bossuyt X, Marti GE, Fleisher TA. Comparative analysis of whole blood lysis methods for flow cytometry. Cytometry 1997;30:124-133.
31. Menendez P, Redondo O, Rodriguez A, Lopez-Berges MC, Ercilla G, Lopez A, Duran A, Almeida J, Perez-Simon JA, San Miguel JF, Gratama JW, Orfao A. Comparison between a lyse-and-then-wash method and a lyse-non-wash technique for the enumeration of CD34+ hematopoietic progenitor cells. Cytometry 1998;34:264-271.
32. Martin RM, Leonhardt H, Cardoso MC. DNA labeling in living cells. Cytometry A 2005;67A:45-52.
33. Yuan CM, Douglas-Nikitin VK, Ahrens KP, Luchetta GR, Braylan RC, Yang L. DRAQ5-based DNA content analysis of hematolymphoid cell subpopulations discriminated by surface antigens and light scatter properties. Cytometry B 2004;58B:47-52.