Flow cytometric validation of automated differentials in pediatric patients

Laboratory Hematology

Volumn 10, Issue 2, 2004, Pages 112-118

  Finn, Laura S ^a   Hall, Janice ^a   Xu, Min ^a   Rutledge, Joe C ^a  

^a UNIVERSITY OF WASHINGTON   (United States)

Author keywords

Automated differential; Flow cytometry; Manual differential

Indexed keywords

ADOLESCENT; ADULT; ARTICLE; AUTOANALYZER; BLOOD SAMPLING; CELL TYPE; CHILD; CONTROLLED STUDY; CORRELATION ANALYSIS; DIAGNOSTIC ACCURACY; DIAGNOSTIC VALUE; FLOW CYTOMETRY; HUMAN; INFANT; INTERMETHOD COMPARISON; LEUKOCYTE DIFFERENTIAL COUNT; MONOCYTE; NEWBORN; STATISTICAL ANALYSIS; STATISTICAL SIGNIFICANCE; VALIDATION PROCESS;

AUTOANALYSIS; CHILD; FLOW CYTOMETRY; HUMANS; LYMPHOCYTE COUNT; MONOCYTES; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY;

EID: 3042725695     PISSN: 10802924     EISSN: None     Source Type: Journal    
DOI: 10.1532/LH96.04027     Document Type: Article

References (18)

1. NCCLS. Reference leukocyte differential count (proportional) and evaluation of instrumental methods. Approved Standard, NCCLS document H20-A (ISBN 1-56238-131-8). 1992.
2. Buttarello M, Gadotti M, Lorenz C, et al. Evaluation of four automated hematology analyzers. A comparative study of differential counts (imprecision and inaccuracy). Am J Clin Pathol. 1992;97: 345-352.
3. Thalhammer-Scherrer R, Knobl P, Korninger L, Schwarzinger I. Atuomated five-part white blood cell differential counts. Efficiency of software-generated white blood cell suspect flags of the hematology analyzers Sysmex SE-9000, Sysmex NE-8000, and Coulter STKS. Arch Pathol Lab Med. 1997;6:573-577.
4. Koenn ME, Kirby BA, Cook LL, et al. Comparison of four automated hematology analyzers. Clin Lab Sci. 2001;14:238-242.
5. Ruzicka K, Veitl M, Thalhammer-Scherrer R, Schwarzinger I. The new hematology analyzer Sysmex XE-2100: performance evaluation of a novel white blood cell differential technology. Arch Pathol Lab Med. 2001;125:391-396.
6. Cornbleet J, Fernades BJ, Miers M. Streamline your automated hematology laboratory: roundtable discussion [part 3]. MLO Med Lab Obs. 1997;29:43, 46-47.
7. Lands KL, Harris RJ, Davis G, Renner N, Finn WG. Elimination of instrument-driven reflex manual differential leukocyte counts. Am J Clin Pathol. 2003;119:656-662.
8. Stelzer GT, Shults KE, Loken MR. CD45 gating for routine flow cytometric analysis of human bone marrow specimens. Ann N Y Acad Sci. 1993;677:265-80.
9. Siekmeier R, Bierlich A, Jaross W. The white blood cell differential: three methods compared. Clin Chem Lab Med. 2001;39:432-445.
10. Drewinko B, Bollinger P, Rountree M, et al. Eight-parameter automated hematology analyzers: comparison of two flow cytometric systems. Am J Clin Pathol. 1982;78:738-747.
11. Vidriales MB, San-Miguel JF, Orfao A, Coustan-Smith E, Campana D. Minimal residual disease monitoring by flow cytometry. Best Pract Res Clin Haematol. 2003;16:599-612.
12. Burchert-Graeve M, Kock R. Automated leucocyte differentials in 292 patients with leucopenia: an evaluation of the Abbott CELL-CYN 3500 (CD3500) haematology analyzer. Clin Lab Haematol. 1196;18:253-259.
13. Parham DM, Ready R, Stine K, Quiggins C, Becton D, North P. Comparison of manual and automated leukocyte counts for determination of the absolute neutrophilic count: application to a pediatric oncology clinic. Med Pediatr Oncol 2002;38:183-186.
14. Lebeck LK, Mast BJ, Houwen B. Flow cytometric white blood cell differentials: a proposed alternate reference method. Sysmex J Int. 1993;3:61-69.
15. Koepke JA, Dotson MA, Shifman MA. A critical evaluation of the manual visual differential leukocyte counting method. Blood Cells. 1985;11:173-186.
16. Hubl W, Hauptlorenz S, Tlustos L, Jilch R, Fishcer M, Bayer PM. Precision and accuracy of monocyte counting. Comparison of two hematology analyzers, the manual differential and flow cytometry. Am J Clin Pathol. 1995;103:167-170.
17. Hubl W, Tlustos L, Erath A, Andert S, Bayer PM. Proposed reference method for peripheral-blood monocyte counting using fluorescence-labelled monoclonal antibodies. Cytometry. 1996;26:69-74.
18. Hubl W, Wolfbauer G, Andert S, et al. Toward a new reference method for the leukocyte five-part differential. Cytometry. 1997;30:72-84.