Leukoflow: Multiparameter extended white blood cell differentiation for routine analysis by flow cytometry

Cytometry Part A

Volumn 79 A, Issue 9, 2011, Pages 694-706

  van de Geijn, Gert Jan ^a   van Rees, Vincent ^a   van Pul Bom, Natasja ^a   Birnie, Erwin ^a   Janssen, Hans ^a   Pegels, Hans ^a   Beunis, Marlène ^a   Njo, Tjin ^a  

^a SINT FRANCISCUS GASTHUIS   (Netherlands)

Author keywords

Cytology; Differentiation; Flow cytometry; Hematology analyzer; Leukocytes; Routine diagnostics; WBC; White blood cell

Indexed keywords

BIOLOGICAL MARKER; LEUKOCYTE ANTIGEN;

ADULT; ARTICLE; CELL DIFFERENTIATION; COMPARATIVE STUDY; CYTOLOGY; ERYTHROBLAST; FLOW CYTOMETRY; HUMAN; LEUKOCYTE; LEUKOCYTE COUNT; METHODOLOGY; MICROSCOPY; REPRODUCIBILITY; STAINING; VALIDATION STUDY;

ADULT; ANTIGENS, CD; BIOLOGICAL MARKERS; CELL DIFFERENTIATION; ERYTHROBLASTS; FLOW CYTOMETRY; HUMANS; LEUKOCYTE COUNT; LEUKOCYTES; MICROSCOPY; REPRODUCIBILITY OF RESULTS; STAINING AND LABELING;

EID: 80051862079     PISSN: 15524922     EISSN: 15524930     Source Type: Journal    
DOI: 10.1002/cyto.a.21105     Document Type: Article

References (28)

1. Pierre RV. Peripheral blood film review. The demise of the eyecount leukocyte differential. Clin Lab Med 2002; 22: 279-297.
2. Ruemke CL. The statistically expected variability in differential leukocytes counting. In: Koepke JA, editor. Differential Leucocytes Counting. CAP Conference Aspen College of American Pathologist; 1977. pp 39-46.
3. Koepke JA, Van Assendelft OW, Brindza LJ, Davis BH, Fernandes BJ, Gewirtz AS, Rabinovitch A. Reference leukocyte (WBC) differential count (Proportional) and evaluation of instrumental methods; Approved Standard-Second Edition. Wayne, Pennsylvania: Clinical and Laboratory Standards Institute; 2007. pp 1-35.
4. Terstappen LW, Loken MR. Five-dimensional flow cytometry as a new approach for blood and bone marrow differentials. Cytometry 1988; 9: 548-556.
5. Chng WJ, Tan GB, Kuperan P. Establishment of adult peripheral blood lymphocyte subset reference range for an Asian population by single-platform flow cytometry: Influence of age, sex, and race and comparison with other published studies. Clin Diagn Lab Immunol 2004; 11: 168-173.
6. Ducrest S, Meier F, Tschopp C, Pavlovic R, Dahinden CA. Flowcytometric analysis of basophil counts in human blood and inaccuracy of hematology analyzers. Allergy 2005; 60: 1446-1450.
7. Fornas O, Domingo JC, Marin P, Petriz J. Flow cytometric-based isolation of nucleated erythroid cells during maturation: An approach to cell surface antigen studies. Cytometry 2002; 50: 305-312.
8. + cells in whole blood. Nat Med 2000; 6: 833-836.
9. Fujimoto H, Sakata T, Hamaguchi Y, Shiga S, Tohyama K, Ichiyama S, Wang FS, Houwen B. Flow cytometric method for enumeration and classification of reactive immature granulocyte populations. Cytometry 2000; 42: 371-378.
10. Costa ES, Arroyo ME, Pedreira CE, Garcia-Marcos MA, Tabernero MD, Almeida J, Orfao A. A new automated flow cytometry data analysis approach for the diagnostic screening of neoplastic B-cell disorders in peripheral blood samples with absolute lymphocytosis. Leukemia 2006; 20: 1221-1230.
11. Hubl W, Wolfbauer G, Andert S, Thum G, Streicher J, Hubner C, Lapin A, Bayer PM. Toward a new reference method for the leukocyte five-part differential. Cytometry 1997; 30: 72-84.
12. Bjornsson S, Wahlstrom S, Norstrom E, Bernevi I, O'Neill U, Johansson E, Runstrom H, Simonsson P. Total nucleated cell differential for blood and bone marrow using a single tube in a five-color flow cytometer. Cytometry Part B Clin Cytom 2008; 74B: 91-103.
13. Faucher JL, Lacronique-Gazaille C, Frebet E, Trimoreau F, Donnard M, Bordessoule D, Lacombe F, Feuillard J. "6 markers/5 colors" extended white blood cell differential by flow cytometry. Cytometry Part A 2007; 71A: 934-944.
14. Cherian S, Levin G, Lo WY, Mauck M, Kuhn D, Lee C, Wood BL. Evaluation of an 8-color flow cytometric reference method for white blood cell differential enumeration. Cytometry Part B Clin Cytom 2010; 78B: 319-328.
15. Cartwright G. Diagnostic Laboratory Hematology. New York: Grune & Stratton; 1968.
16. Martin RM, Leonhardt H, Cardoso MC. DNA labeling in living cells. Cytometry Part A 2005; 67A: 45-52.
17. Cornbleet PJ, Gochman N. Incorrect least-squares regression coefficients in method-comparison analysis. Clin Chem 1979; 25: 432-438.
18. + blood monocytes: Their role in infection and inflammation. J Leukoc Biol 2007; 81: 584-592.
19. + blood monocytes is expanded in sepsis patients. Blood 1993; 82: 3170-3176.
20. Cornbleet PJ. Clinical utility of the band count. Clin Lab Med 2002; 22: 101-36.
21. van der Meer W, van Gelder W, de Keijzer R, Willems H. Does the band cell survive the 21st century? Eur J Haematol 2006; 76: 251-254.
22. Craig FE, Foon KA. Flow cytometric immunophenotyping for hematologic neoplasms. Blood 2008; 111: 3941-3967.
23. Comans-Bitter WM, de Groot R, van den Beemd R, Neijens HJ, Hop WC, Groeneveld K, Hooijkaas H, van Dongen JJ. Immunophenotyping of blood lymphocytes in childhood. Reference values for lymphocyte subpopulations. J Pediatr 1997; 130: 388-393.
24. Roussel M, Benard C, Ly-Sunnaram B, Fest T. Refining the white blood cell differential: The first flow cytometry routine application. Cytometry Part A 2010; 77A: 552-563.
25. Hansen S, Dahl R, Hoffmann HJ. Determination of blood leukocyte concentration with constant volume acquisition on a flow cytometer is comparable to individualized single platform testing with beads as internal reference standard. J Immunol Methods 2008; 338: 58-62.
26. Briggs C, Longair I, Slavik M, Thwaite K, Mills R, Thavaraja V, Foster A, Romanin D, Machin SJ. Can automated blood film analysis replace the manual differential? An evaluation of the CellaVision DM96 automated image analysis system. Int J Lab Hematol 2009; 31: 48-60.
27. Ceelie H, Dinkelaar RB, van Gelder W. Examination of peripheral blood films using automated microscopy: Evaluation of Diffmaster Octavia and Cellavision DM96. J Clin Pathol 2007; 60: 72-79.
28. Kratz A, Bengtsson HI, Casey JE, Keefe JM, Beatrice GH, Grzybek DY, Lewandrowski KB, Van Cott EM. Performance evaluation of the CellaVision DM96 system: WBC differentials by automated digital image analysis supported by an artificial neural network. Am J Clin Pathol 2005; 124: 770-781.