Visualization of lipid raft membrane compartmentalization in living RN46A neuronal cells using single quantum dot tracking

ACS Chemical Neuroscience

Volumn 3, Issue 10, 2012, Pages 737-743

  Chang, Jerry C ^a   Rosenthal, Sandra J ^a,b,c  

^a VANDERBILT UNIVERSITY   (United States)

^b VANDERBILT UNIVERSITY   (United States)

^c OAK RIDGE NATIONAL LABORATORY   (United States)

Author keywords

lipid rafts; membrane dynamics; Quantum dot; single molecule imaging; trafficking

Indexed keywords

CHOLERA TOXIN B SUBUNIT; GANGLIOSIDE; QUANTUM DOT;

ARTICLE; CELL COMPARTMENTALIZATION; CELL MEMBRANE; DIFFUSION; HUMAN; HUMAN CELL; LIFESPAN; LIPID RAFT; NERVE CELL; PRIORITY JOURNAL; PROTEIN BINDING; SIGNAL TRANSDUCTION;

ANIMALS; CELL COMPARTMENTATION; CELL LINE, TRANSFORMED; G(M1) GANGLIOSIDE; MEMBRANE MICRODOMAINS; NEURONS; QUANTUM DOTS; RATS;

EID: 84867635298     PISSN: None     EISSN: 19487193     Source Type: Journal    
DOI: 10.1021/cn3000845     Document Type: Article

References (37)

1. Munro, S. (2003) Lipid rafts: Elusive or illusive? Cell 115, 377-388 (Pubitemid 37456801)
2. Allen, J. A., Halverson-Tamboli, R. A., and Rasenick, M. M. (2007) Lipid raft microdomains and neurotransmitter signalling Nat. Rev. Neurosci. 8, 128-140 (Pubitemid 46146951)
3. Lingwood, D. and Simons, K. (2010) Lipid rafts as a membrane-organizing principle Science 327, 46-50
4. Korade, Z. and Kenworthy, A. K. (2008) Lipid rafts, cholesterol, and the brain Neuropharmacology 55, 1265-1273
5. Lai, E. C. (2003) Lipid rafts make for slippery platforms J. Cell Biol. 162, 365-370 (Pubitemid 36988545)
6. Vrljic, M., Nishimura, S. Y., and Moerner, W. E. (2004) Strengths and weaknesses of single-molecule tracking. Biophysical society discussions speaker paper 13, SP13 A-J.
7. Joo, C., Balci, H., Ishitsuka, Y., Buranachai, C., and Ha, T. (2008) Advances in single-molecule fluorescence methods for molecular biology Annu. Rev. Biochem. 77, 51-76
8. Sako, Y. and Yanagida, T. (2003) Single-molecule visualization in cell biology Nat. Rev. Mol. Cell Biol. SS1-SS5
9. Rosenthal, S. J., Chang, J. C., Kovtun, O., McBride, J. R., and Tomlinson, I. D. (2011) Biocompatible quantum dots for biological applications Chem. Biol. 18, 10-24
10. Chang, J. C., Kovtun, O., Blakely, R. D., and Rosenthal, S. J. (2012) Labeling of neuronal receptors and transporters with quantum dots Wiley Interdiscip. Rev.: Nanomed. Nanobiotechnol. 10.1002/wnan.1186
11. Dahan, M., Levi, S., Luccardini, C., Rostaing, P., Riveau, B., and Triller, A. (2003) Diffusion dynamics of glycine receptors revealed by single-quantum dot tracking Science 302, 442-445 (Pubitemid 37296269)
12. Cui, B., Wu, C., Chen, L., Ramirez, A., Bearer, E. L., Li, W.-P., Mobley, W. C., and Chu, S. (2007) One at a time, live tracking of NGF axonal transport using quantum dots Proc. Natl. Acad. Sci. U.S.A. 104, 13666-13671 (Pubitemid 350003378)
13. Bouzigues, C., Morel, M., Triller, A., and Dahan, M. (2007) Asymmetric redistribution of GABA receptors during GABA gradient sensing by nerve growth cones analyzed by single quantum dot imaging Proc. Natl. Acad. Sci. U.S.A. 104, 11251-11256 (Pubitemid 47175127)
14. Chang, J. C., Tomlinson, I. D., Warnement, M. R., Ustione, A., Carneiro, A. M. D., Piston, D. W., Blakely, R. D., and Rosenthal, S. J. (2012) Single molecule analysis of serotonin transporter regulation using antagonist- conjugated quantum dots reveals restricted, p38 MAPK-dependent mobilization underlying uptake activation J. Neurosci. 32, 8919-8929
15. Ralf, W., Bernhard, Z., and Michael, K. (2006) High-speed confocal fluorescence imaging with a novel line scanning microscope J. Biomed. Opt. 11 064011
16. Nirmal, M., Dabbousi, B. O., Bawendi, M. G., Macklin, J. J., Trautman, J. K., Harris, T. D., and Brus, L. E. (1996) Fluorescence intermittency in single cadmium selenide nanocrystals Nature 383, 802-804 (Pubitemid 26372658)
17. Yao, J., Larson, D. R., Vishwasrao, H. D., Zipfel, W. R., and Webb, W. W. (2005) Blinking and nonradiant dark fraction of water-soluble quantum dots in aqueous solution Proc. Natl. Acad. Sci. U.S.A. 102, 14284-14289 (Pubitemid 41429690)
18. Schmidt, T., Schutz, G. J., Baumgartner, W., Gruber, H. J., and Schindler, H. (1996) Imaging of single molecule diffusion Proc. Natl. Acad. Sci. U.S.A. 93, 2926-2929 (Pubitemid 26114340)
19. Cheezum, M. K., Walker, W. F., and Guilford, W. H. (2001) Quantitative comparison of algorithms for tracking single fluorescent particles Biophys. J. 81, 2378-2388 (Pubitemid 32917184)
20. Bentzen, E. L., Tomlinson, I. D., Mason, J., Gresch, P., Warnement, M. R., Wright, D., Sanders-Bush, E., Blakely, R., and Rosenthal, S. J. (2005) Surface modification to reduce nonspecific binding of quantum dots in live cell assays Bioconjugate Chem. 16, 1488-1494 (Pubitemid 41681516)
21. Warnement, M. R., Tomlinson, I. D., Chang, J. C., Schreuder, M. A., Luckabaugh, C. M., and Rosenthal, S. J. (2008) Controlling the reactivity of ampiphilic quantum dots in biological assays through hydrophobic assembly of custom PEG derivatives Bioconjugate Chem. 19, 1404-1413 (Pubitemid 352032340)
22. Orlandi, P. A. and Fishman, P. H. (1998) Filipin-dependent inhibition of cholera toxin: evidence for toxin internalization and activation through caveolae-like domains J. Cell Biol. 141, 905-915 (Pubitemid 28243959)
23. Kenworthy, A. K., Petranova, N., and Edidin, M. (2000) High-resolution FRET microscopy of cholera toxin B-subunit and GPI-anchored proteins in cell plasma membranes Mol. Biol. Cell 11, 1645-1655 (Pubitemid 30307902)
24. Daumas, F., Destainville, N., Millot, C., Lopez, A., Dean, D., and Salome, L. (2003) Confined diffusion without fences of a G-protein-coupled receptor as revealed by single particle tracking Biophys. J. 84, 356-366 (Pubitemid 36026404)
25. Saxton, M. J. and Jacobson, K. (1997) Single-particle tracking: Applications to membrane dynamics Annu. Rev. Biophys. Biomol. Struct. 26, 373-399 (Pubitemid 27255378)
26. Murcia, M. J., Minner, D. E., Mustata, G.-M., Ritchie, K., and Naumann, C. A. (2008) Design of quantum dot-conjugated lipids for long-term, high-speed tracking experiments on cell surfaces J. Am. Chem. Soc. 130, 15054-15062
27. Schutz, G. J., Kada, G., Pastushenko, V. P., and Schindler, H. (2000) Properties of lipid microdomains in a muscle cell membrane visualized by single molecule microscopy EMBO J. 19, 892-901 (Pubitemid 30119815)
28. Eggeling, C., Ringemann, C., Medda, R., Schwarzmann, G., Sandhoff, K., Polyakova, S., Belov, V. N., Hein, B., von Middendorff, C., Schonle, A., and Hell, S. W. (2009) Direct observation of the nanoscale dynamics of membrane lipids in a living cell Nature 457, 1159-1162
29. Andrews, N. L., Lidke, K. A., Pfeiffer, J. R., Burns, A. R., Wilson, B. S., Oliver, J. M., and Lidke, D. S. (2008) Actin restricts FcepsilonRI diffusion and facilitates antigen-induced receptor immobilization Nat. Cell Biol. 10, 955-963
30. Fabien, P., Xavier, M., Gopal, I., Emmanuel, M., Hsiao-Ping, M., and Shimon, W. (2009) Dynamic partitioning of a glycosyl-phosphatidylinositol- anchored protein in glycosphingolipid-rich microdomains imaged by single-quantum dot tracking Traffic 10, 691-712
31. Burli, T., Baer, K., Ewers, H., Sidler, C., Fuhrer, C., and Fritschy, J. M. (2010) Single particle tracking of alpha7 nicotinic AChR in hippocampal neurons reveals regulated confinement at glutamatergic and GABAergic perisynaptic sites PLoS One 5 e11507
32. Groc, L., Lafourcade, M., Heine, M., Renner, M., Racine, V., Sibarita, J. B., Lounis, B., Choquet, D., and Cognet, L. (2007) Surface trafficking of neurotransmitter receptor: comparison between single-molecule/quantum dot strategies J. Neurosci. 27, 12433-12437 (Pubitemid 350127795)
33. Wieser, S. and Schutz, G. J. (2008) Tracking single molecules in the live cell plasma membrane-Do's and Don't's Methods 46, 131-140
34. Lidke, D. S., Nagy, P., Heintzmann, R., Arndt-Jovin, D. J., Post, J. N., Grecco, H. E., Jares-Erijman, E. A., and Jovin, T. M. (2004) Quantum dot ligands provide new insights into erbB/HER receptor-mediated signal transduction Nat. Biotechnol. 22, 198-203 (Pubitemid 38160535)
35. White, L. A., Eaton, M. J., Castro, M. C., Klose, K. J., Globus, M. Y., Shaw, G., and Whittemore, S. R. (1994) Distinct regulatory pathways control neurofilament expression and neurotransmitter synthesis in immortalized serotonergic neurons J. Neurosci. 14, 6744-6753 (Pubitemid 24333092)
36. Jaqaman, K., Loerke, D., Mettlen, M., Kuwata, H., Grinstein, S., Schmid, S. L., and Danuser, G. (2008) Robust single-particle tracking in live-cell time-lapse sequences Nat. Methods 5, 695-702
37. Martin, D. S., Forstner, M. B., and Kas, J. A. (2002) Apparent subdiffusion inherent to single particle tracking Biophys. J. 83, 2109-2117