Expression, purification and antimicrobial activity of puroindoline Aprotein and its mutants

Amino Acids

Volumn 43, Issue 4, 2012, Pages 1689-1696

  Miao, Yingjie ^a   Chen, Ling ^a,b   Wang, Cheng ^a   Wang, Yajuan ^a   Zheng, Qian ^a   Gao, Chunbao ^b   Yang, Guangxiao ^a   He, Guangyuan ^a  

^a HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY   (China)

^b INSTITUTE OF PLANT PROTECTION   (China)

Author keywords

Antimicrobial activity; Artificial mutants; Circular dichroism; Minimum inhibitory concentration; Puroindoline A; Tryptophan rich domain

Indexed keywords

ANTIINFECTIVE AGENT; PROTEIN ABBBC; PROTEIN ABBC; PUROINDOLINE A; TRYPTOPHAN; UNCLASSIFIED DRUG;

AFFINITY CHROMATOGRAPHY; ANTIMICROBIAL ACTIVITY; ARTICLE; CIRCULAR DICHROISM; CONTROLLED STUDY; ESCHERICHIA COLI; EXPRESSION VECTOR; FLUORESCENCE MICROSCOPY; METAL AFFINITY CHROMATOGRAPHY; MINIMUM INHIBITORY CONCENTRATION; MUTANT; NONHUMAN; ONLINE SYSTEM; PREDICTION; PRIORITY JOURNAL; PROTEIN CLEAVAGE; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN PURIFICATION; PROTEIN SECONDARY STRUCTURE; PROTEIN TERTIARY STRUCTURE; STAPHYLOCOCCUS AUREUS;

AMINO ACID SEQUENCE; ANTI-BACTERIAL AGENTS; CIRCULAR DICHROISM; CLONING, MOLECULAR; ESCHERICHIA COLI; GENE EXPRESSION; GENETIC ENGINEERING; MICROBIAL SENSITIVITY TESTS; MICROBIAL VIABILITY; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTATION; PLANT PROTEINS; PROTEIN STRUCTURE, SECONDARY; PROTEIN STRUCTURE, TERTIARY; RECOMBINANT FUSION PROTEINS; STAPHYLOCOCCUS AUREUS; STRUCTURE-ACTIVITY RELATIONSHIP; TRITICUM; TRYPTOPHAN;

STAPHYLOCOCCUS AUREUS; TRITICUM AESTIVUM;

EID: 84867579440     PISSN: 09394451     EISSN: 14382199     Source Type: Journal    
DOI: 10.1007/s00726-012-1250-x     Document Type: Article

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