Construction of plasmids containing site-specific DNA interstrand cross-links for biochemical and cell biological studies

Methods in Molecular Biology

Volumn 920, Issue , 2012, Pages 203-219

  Enoiu, Milica ^a   Ho, The Vinh ^b   Long, David T ^c   Walter, Johannes C ^c   Schärer, Orlando D ^b  

^a UNIVERSITY OF ZURICH   (Switzerland)

^b STONY BROOK UNIVERSITY   (United States)

^c HARVARD MEDICAL SCHOOL   (United States)

Author keywords

BbsI restriction enzyme; Gel filtration; Interstrand cross link; Ligation; Plasmid DNA substrate; Site specific damage

Indexed keywords

EID: 84867531801     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-61779-998-3_15     Document Type: Article

References (13)

1. Noll DM, Mason TM, Miller PS (2006) Formation and repair of interstrand cross-links in DNA. Chem Rev 106: 277-301
2. Schärer OD (2005) DNA interstrand crosslinks: natural and drug-induced DNA adducts that induce unique cellular responses. Chembiochem 6: 27-32 (Pubitemid 40128631)
3. Guainazzi A, Schärer OD (2010) Using synthetic DNA interstrand crosslinks to elucidate repair pathways and identify new therapeutic targets for cancer chemotherapy. Cell Mol Life Sci 67: 3683-3697
4. Knipscheer P, Räschle M, Smogorzewska A, Enoiu M, Ho TV, Schärer OD, Elledge SJ, Walter JC (2009) The fanconi anemia pathway promotes replication-dependent DNA inter-strand cross-link repair. Science 326: 1698-1701
5. Räschle M, Knipsheer P, Enoiu M, Angelov T, Sun J, Griffith JD, Ellenberger TE, Schärer OD, Walter JC (2008) Mechanism of replication-coupled DNA interstrand crosslink repair. Cell 134: 969-980
6. Zhang N, Lu X, Zhang X, Peterson CA, Legerski RJ (2002) hMutSbeta is required for the recognition and uncoupling of psoralen interstrand cross-links in vitro. Mol Cell Biol 22: 2388-2397 (Pubitemid 34224641)
7. Shen X, Do H, Li Y, Chung WH, Tomasz M, de Winter JP, Xia B, Elledge SJ, Wang W, Li L (2009) Recruitment of fanconi anemia and breast cancer proteins to DNA damage sites is differentially governed by replication. Mol Cell 35: 716-723
8. Shen X, Jun S, O'Neal LE, Sonoda E, Bemark M, Sale JE, Li L (2006) REV3 and REV1 play major roles in recombination-independent repair of DNA interstrand cross-links mediated by monoubiquitinated proliferating cell nuclear antigen (PCNA). J Biol Chem 281: 13869-13872 (Pubitemid 43848310)
9. Wang X, Peterson CA, Zheng H, Nairn RS, Legerski RJ, Li L (2001) Involvement of nucle-otide excision repair in a recombination-independent and error-prone pathway of DNA interstrand cross-link repair. Mol Cell Biol 21: 713-720 (Pubitemid 32104720)
10. Zheng H, Wang X, Warren AJ, Legerski RJ, Nairn RS, Hamilton JW, Li L (2003) Nucleotide excision repair-and polymerase eta-mediated error-prone removal of mitomy-cin C interstrand cross-links. Mol Cell Biol 23: 754-761 (Pubitemid 36050566)
11. Hofr C, Brabec V (2001) Thermal and ther-modynamic properties of duplex DNA containing site-specific interstrand cross-link of antitumor cisplatin or its clinically ineffective trans isomer. J Biol Chem 276: 9655-9661
12. Angelov T, Guainazzi A, Schärer OD (2009) Generation of DNA interstrand cross-links by post-synthetic reductive amination. Org Lett 11: 661-664
13. Guainazzi A, Campbell AJ, Angelov T, Simmerling C, Schärer OD (2010) Synthesis and molecular modeling of a nitrogen mustard DNA interstrand crosslink. Chemistry 16: 12100-12103