Mechanistic studies of semicarbazone triapine targeting human ribonucleotide reductase in vitro and in mammalian cells: Tyrosyl radical quenching not involving reactive oxygen species

Journal of Biological Chemistry

Volumn 287, Issue 42, 2012, Pages 35768-35778

  Aye, Yimon ^a   Long, Marcus J C ^b   Stubbe, JoAnne ^a  

^a MASSACHUSETTS INSTITUTE OF TECHNOLOGY   (United States)

^b BRANDEIS UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AEROBIC AND ANAEROBIC CONDITIONS; BIOCHEMICAL STUDIES; CELLULAR TARGETS; COFACTORS; IN-PHASE; IN-SITU; IN-VITRO; IRON CHELATION; KINETIC DATA; LYSATES; MAMMALIAN CELL LINES; MAMMALIAN CELLS; MASS SPECTROMETRIC ANALYSIS; MECHANISTIC STUDIES; REACTIVE OXYGEN SPECIES; REDUCTANTS; RIBONUCLEOTIDE REDUCTASE; SELECTIVE DEGRADATION; SEMICARBAZONES; THIOSEMICARBAZONES; TYROSYL RADICALS; WHOLE CELL;

BIOCHEMISTRY; CELL CULTURE; IRON;

OXYGEN;

3 AMINOPICOLINALDEHYDE THIOSEMICARBAZONE; IRON; REACTIVE OXYGEN METABOLITE; RIBONUCLEOTIDE REDUCTASE; YTTRIUM;

ANIMAL CELL; ARTICLE; CELL LABELING; CELL LEVEL; COMPLEX FORMATION; CONTROLLED STUDY; DRUG INHIBITION; DRUG TARGETING; ENZYME ACTIVITY; ENZYME INACTIVATION; ENZYME INHIBITION; ENZYME KINETICS; HUMAN; IN VITRO STUDY; IRON CHELATION; MAMMAL CELL; MOLECULAR MECHANICS; NONHUMAN; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN DEPLETION; PROTEIN STABILITY; PROTEIN TARGETING;

ANIMALS; CERCOPITHECUS AETHIOPS; COS CELLS; ENZYME STABILITY; HUMANS; HYDROGEN PEROXIDE; IRON; K562 CELLS; MICE; NIH 3T3 CELLS; OXIDATION-REDUCTION; PROTEIN SUBUNITS; PYRIDINES; RIBONUCLEOTIDE REDUCTASES; SUPEROXIDES; THIOSEMICARBAZONES;

EID: 84867420385     PISSN: 00219258     EISSN: 1083351X     Source Type: Journal    
DOI: 10.1074/jbc.M112.396911     Document Type: Article

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