Lentiviral-Mediated RNAi Knockdown of Cbfa1 Gene Inhibits Endochondral Ossification of Antler Stem Cells in Micromass Culture

PLoS ONE

Volumn 7, Issue 10, 2012, Pages

  Sun, Hongmei ^a,c   Yang, Fuhe ^a,c   Chu, Wenhui ^a,c   Zhao, Haiping ^a,c   McMahon, Chris ^b   Li, Chunyi ^b,c  

^a INSTITUTE OF PLANT PROTECTION   (China)

^b INVERMAY AGRICULTURAL CENTRE   (New Zealand)

^c State Key Laboratory for Molecular Biology of Special Economic Animals   (China)

Author keywords

[No Author keywords available]

Indexed keywords

COLLAGEN TYPE 1; LENTIVIRUS VECTOR; OSTEOCALCIN; TRANSCRIPTION FACTOR RUNX2;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; ARTICULAR CARTILAGE; CBFA1 GENE; CELL CULTURE; CELL DIFFERENTIATION; CHONDROGENESIS; CONTROLLED STUDY; DEER; DOWN REGULATION; ENCHONDRAL OSSIFICATION; ENZYME REPRESSION; GENE; GENE EXPRESSION; GENE FUNCTION; GENE REPRESSION; GENE SILENCING; GENE SWITCHING; GROWTH INHIBITION; HORN; IN VITRO STUDY; LENTIVIRINAE; MALE; MOLECULAR MECHANICS; NONHUMAN; PHENOTYPE; RNA INTERFERENCE; SIGNAL TRANSDUCTION; STEM CELL; TISSUE REGENERATION; TISSUE REPAIR; VASCULARIZATION;

ANIMALS; ANTLERS; BLOTTING, WESTERN; COLLAGEN TYPE I; CORE BINDING FACTOR ALPHA 1 SUBUNIT; IMMUNOHISTOCHEMISTRY; LENTIVIRUS; MALE; OSTEOCALCIN; OSTEOGENESIS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA INTERFERENCE; STEM CELLS;

EID: 84867300931     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0047367     Document Type: Article

References (56)

1. Newman AP, (1998) Articular cartilage repair. Am J Sports Med 26: 309-324.
2. Khan IM, Gilbert SJ, Singhrao SK, Duance VC, Archer CW, (2008) Cartilage integration: evaluation of the reasons for failure of integration during cartilage repair. A review. Eur Cell Mater 16: 26-39.
3. Shapiro F, Koide S, Glimcher MJ, (1993) Cell origin and differentiation in the repair of full-thickness defects of articular cartilage. J Bone Joint Surg Am 75: 532-553.
4. Amiel D, Coutts RD, Abel M, Stewart W, Harwood F, et al. (1985) Rib perichondrial grafts for the repair of full-thickness articular-cartilage defects. A morphological and biochemical study in rabbits. J Bone Joint Surg Am 67: 911-920.
5. O'Driscoll SW, Salter RB (1986) The repair of major osteochondral defects in joint surfaces by neochondrogenesis with autogenous osteoperiosteal grafts stimulated by continuous passive motion. An experimental investigation in the rabbit. Clin Orthop Relat Res: 131-140.
6. Frenkel SR, Di Cesare PE, (2004) Scaffolds for articular cartilage repair. Ann Biomed Eng 32: 26-34.
7. Khan IM, Palmer EA, Archer CW, (2010) Fibroblast growth factor-2 induced chondrocyte cluster formation in experimentally wounded articular cartilage is blocked by soluble Jagged-1. Osteoarthritis Cartilage 18: 208-219.
8. Di Cesare PE, Frenkel SR, Carlson CS, Fang C, Liu C, (2006) Regional gene therapy for full-thickness articular cartilage lesions using naked DNA with a collagen matrix. J Orthop Res 24: 1118-1127.
9. Ahmed TA, Hincke MT, (2010) Strategies for articular cartilage lesion repair and functional restoration. Tissue Eng Part B Rev 16: 305-329.
10. Becerra J, Andrades JA, Guerado E, Zamora-Navas P, Lopez-Puertas JM, et al. (2010) Articular cartilage: structure and regeneration. Tissue Eng Part B Rev 16: 617-627.
11. Chu CR, Szczodry M, Bruno S, (2010) Animal models for cartilage regeneration and repair. Tissue Eng Part B Rev 16: 105-115.
12. Goss RJ (1983) Deer Antlers. Regeneration, Function and Evolution. New York, NY: Academic Press.
13. Banks WJ, Newbrey JW (1982) Light microscopic studies of the ossification process in developing antlers. In: Brown RD, editor. Caesar Kleberg Wildl. Res. Inst., Kingsville, Texas. 231-260.
14. Li C, Suttie JM, (1994) Light microscopic studies of pedicle and early first antler development in red deer (Cervus elaphus). Anat Rec 239: 198-215.
15. Li C, Mackintosh CG, Martin SK, Clark DE, (2007) Identification of key tissue type for antler regeneration through pedicle periosteum deletion. Cell Tissue Res 328: 65-75.
16. Kierdorf U, Stoffels E, Stoffels D, Kierdorf H, Szuwart T, et al. (2003) Histological studies of bone formation during pedicle restoration and early antler regeneration in roe deer and fallow deer. Anat Rec 273A: 741-751.
17. Li C, Suttie JM, Clark DE, (2005) Histological examination of antler regeneration in red deer (Cervus elaphus). Anat Rec A Discov Mol Cell Evol Biol 282: 163-174.
18. Berg DK, Li C, Asher G, Wells DN, Oback B, (2007) Red deer cloned from antler stem cells and their differentiated progeny. Biol Reprod 77: 384-394.
19. Li C, Yang F, Sheppard A, (2009) Adult stem cells and mammalian epimorphic regeneration-insights from studying annual renewal of deer antlers. Curr Stem Cell Res Ther 4: 237-251.
20. Banks WJ, Newbrey JW (1982) Antler development as a unique modification of mammalian endochondral ossification. In: Brown RD, editor. Caesar Kleberg Wildl. Res. Inst., Kingsville, Texas. 279-306.
21. Banks W, (1973) Histological and ultrastructural aspects of cervine antler development. Anat Rec 175: 487.
22. Frasier MB, Banks WJ, (1973) Characterization of antler mucosubstances by selected histochemical techniques. Anat Rec 175: 323.
23. Li C, Waldrup KA, Corson ID, Littlejohn RP, Suttie JM, (1995) Histogenesis of antlerogenic tissues cultivated in diffusion chambers in vivo in red deer (Cervus elaphus). J Exp Zool 272: 345-355.
24. Li C, Harris AJ, Suttie JM, (2001) Tissue interactions and antlerogenesis: new findings revealed by a xenograft approach. J Exp Zool 290: 18-30.
25. Wang W, Lord E, Li C, Clark D (2004) Use of micromass cell culture for a chondrogenic bioassay. In: Eaton-Rye J, editor. Queenstown, New Zealand. 91.
26. Lian JB, Stein GS, (2003) Runx2/Cbfa1: a multifunctional regulator of bone formation. Curr Pharm Des 9: 2677-2685.
27. Komori T, Yagi H, Nomura S, Yamaguchi A, Sasaki K, et al. (1997) Targeted disruption of Cbfa1 results in a complete lack of bone formation owing to maturational arrest of osteoblasts. Cell 89: 755-764.
28. Enomoto H, Enomoto-Iwamoto M, Iwamoto M, Nomura S, Himeno M, et al. (2000) Cbfa1 is a positive regulatory factor in chondrocyte maturation. J Biol Chem 275: 8695-8702.
29. Ducy P, (2000) Cbfa1: a molecular switch in osteoblast biology. Dev Dyn 219: 461-471.
30. Leung RK, Whittaker PA, (2005) RNA interference: from gene silencing to gene-specific therapeutics. Pharmacol Ther 107: 222-239.
31. Li C, Suttie JM, (2003) Tissue collection methods for antler research. Eur J Morphol 41: 23-30.
32. Li C, Littlejohn RP, Suttie JM, (1999) Effects of insulin-like growth factor 1 and testosterone on the proliferation of antlerogenic cells in vitro. J Exp Zool 284: 82-90.
33. Mello MA, Tuan RS, (1999) High density micromass cultures of embryonic limb bud mesenchymal cells: an in vitro model of endochondral skeletal development. In Vitro Cell Dev Biol Anim 35: 262-269.
34. Chu W, Zhao H, Yang F, Xing X, Liu G, et al. (2009) Construction and characterisation of lentiviral vector of RNA interference of collagen type X of sika deer. Journal of China Agricultural University 14: 29-34.
35. Does RJ, Strijbosch LW, Albers W, (1988) Using jackknife methods for estimating the parameter in dilution series. Biometrics 44: 1093-1102.
36. Burnette WN, (1981) "Western blotting".: electrophoretic transfer of proteins from sodium dodecyl sulfate - polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A. Anal Biochem 112: 195-203.
37. Li C, Gao X, Yang F, Martin SK, Haines SR, et al. (2009) Development of a nude mouse model for the study of antlerogenesis - mechanism of tissue interactions and ossification pathway. J Exp Zool B Mol Dev Evol 312: 118-135.
38. Zhang L, Su P, Xu C, Yang J, Yu W, et al. (2010) Chondrogenic differentiation of human mesenchymal stem cells: a comparison between micromass and pellet culture systems. Biotechnol Lett 32: 1339-1346.
39. Gartel AL, Kandel ES, (2006) RNA interference in cancer. Biomol Eng 23: 17-34.
40. Abbas-Terki T, Blanco-Bose W, Deglon N, Pralong W, Aebischer P, (2002) Lentiviral-mediated RNA interference. Hum Gene Ther 13: 2197-2201.
41. Yang J, Xu J, Zhong X, Zhang Y, Wu Q, et al. (2009) An advanced blue-white screening method for construction of shRNA expression vectors. Biochem Biophys Res Commun 390: 97-102.
42. Elbashir S, Harborth J, Lendeckel W, Yalcin A, Klaus W, et al. (2001) Duplexes of 21-nucleotide RNAs mediate RNA interference in mammalian cell culture. Nature 411: 494-498.
43. Doschak MR, Cooper DM, Huculak CN, Matyas JR, Hart DA, et al. (2003) Angiogenesis in the distal femoral chondroepiphysis of the rabbit during development of the secondary centre of ossification. J Anat 203: 223-233.
44. Clark DE, Li C, Wang W, Martin SK, Suttie JM, (2006) Vascular localization and proliferation in the growing tip of the deer antler. Anat Rec A Discov Mol Cell Evol Biol 288: 973-981.
45. Li C, Clark DE, Lord EA, Stanton JA, Suttie JM, (2002) Sampling technique to discriminate the different tissue layers of growing antler tips for gene discovery. Anat Rec 268: 125-130.
46. Lord EA, Martin SK, Gray JP, Li C, Clark DE, (2007) Cell cycle genes PEDF and CDKN1C in growing deer antlers. Anat Rec (Hoboken) 290: 994-1004.
47. Stockwell R (1979) Biology of Cartilage Cells. London: Cambridge University Press.
48. Li C, Littlejohn RP, Corson ID, Suttie JM, (2003) Effects of testosterone on pedicle formation and its transformation to antler in castrated male, freemartin and normal female red deer (Cervus elaphus). Gen Comp Endocrinol 131: 21-31.
49. Suttie JM, Fennessy PF, Crosbie SF, Corson ID, Laas FJ, et al. (1991) Temporal changes in LH and testosterone and their relationship with the first antler in red deer (Cervus elaphus) stags from 3 to 15 months of age. J Endocrinol 131: 467-474.
50. Suttie JM, Gluckman PD, Butler JH, Fennessy PF, Corson ID, et al. (1985) Insulin-like growth factor 1 (IGF-1) antler-stimulating hormone? Endocrinology 116: 846-848.
51. Kirsch T, von der Mark K, (1990) Isolation of bovine type X collagen and immunolocalization in growth-plate cartilage. Biochem J 265: 453-459.
52. Marriott A, Ayad S, Grant ME, (1991) The synthesis of type X collagen by bovine and human growth-plate chondrocytes. J Cell Sci 99 (Pt 3): 641-649.
53. Price JS, Oyajobi BO, Nalin AM, Frazer A, Russell RG, et al. (1996) Chondrogenesis in the regenerating antler tip in red deer: expression of collagen types I, IIA, IIB, and X demonstrated by in situ nucleic acid hybridization and immunocytochemistry. Dev Dyn 205: 332-347.
54. Iyama K, Ninomiya Y, Olsen BR, Linsenmayer TF, Trelstad RL, et al. (1991) Spatiotemporal pattern of type X collagen gene expression and collagen deposition in embryonic chick vertebrae undergoing endochondral ossification. Anat Rec 229: 462-472.
55. Inada M, Yasui T, Nomura S, Miyake S, Deguchi K, et al. (1999) Maturational disturbance of chondrocytes in Cbfa1-deficient mice. Dev Dyn 214: 279-290.
56. Stricker S, Fundele R, Vortkamp A, Mundlos S, (2002) Role of Runx genes in chondrocyte differentiation. Dev Biol 245: 95-108.