Determining Biophysical Protein Stability in Lysates by a Fast Proteolysis Assay, FASTpp

PLoS ONE

Volumn 7, Issue 10, 2012, Pages

  Minde, David P ^a   Maurice, Madelon M ^b   Rüdiger, Stefan G D ^a  

^a UTRECHT UNIVERSITY   (Netherlands)

^b UNIVERSITY MEDICAL CENTER UTRECHT   (Netherlands)

Author keywords

[No Author keywords available]

Indexed keywords

LIGAND; PROTEIN;

ARTICLE; ASSAY; BIOMEDICINE; BIOPHYSICS; CELL LYSATE; FAST PARALLEL PROTEOLYSIS ASSAY; FLUORESCENCE ANALYSIS; POINT MUTATION; PROTEIN CLEAVAGE; PROTEIN DEGRADATION; PROTEIN DOMAIN; PROTEIN ENGINEERING; PROTEIN FOLDING; PROTEIN INTERACTION; PROTEIN STABILITY; PROTEIN UNFOLDING; REACTION TIME; TEMPERATURE; VALIDATION PROCESS;

AMINOACYLTRANSFERASES; BACTERIAL PROTEINS; BIOPHYSICAL PHENOMENA; CHEMISTRY TECHNIQUES, ANALYTICAL; CYSTEINE ENDOPEPTIDASES; CYTOCHROMES C; ELECTROPHORESIS, POLYACRYLAMIDE GEL; HEME; KINETICS; MALTOSE; MALTOSE-BINDING PROTEINS; POINT MUTATION; PROTEIN BINDING; PROTEIN DENATURATION; PROTEIN FOLDING; PROTEIN STABILITY; PROTEIN STRUCTURE, TERTIARY; PROTEIN UNFOLDING; PROTEINS; PROTEOLYSIS; REPRODUCIBILITY OF RESULTS; TEMPERATURE; THERMOLYSIN;

EID: 84867090696     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0046147     Document Type: Article

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