Novel polyoxins generated by heterologously expressing polyoxin biosynthetic gene cluster in the sanN inactivated mutant of Streptomyces ansochromogenes

Microbial Cell Factories

Volumn 11, Issue , 2012, Pages

  Li, Jine ^a   Li, Lei ^a   Feng, Chi ^a,b   Chen, Yihua ^a   Tan, Huarong ^a  

^a INSTITUTE OF MICROBIOLOGY   (China)

^b UNIVERSITY OF CHINESE ACADEMY OF SCIENCES   (China)

Author keywords

Gene cluster; Heterologous expression; Polyoxin; S. ansochromogenes

Indexed keywords

ANTIFUNGAL AGENT; NIKKOMYCIN; POLYNIK A; POLYOXIN N; POLYOXIN O; POLYOXIN P; THYMINE; UNCLASSIFIED DRUG;

ANTIFUNGAL ACTIVITY; ARTICLE; BIOASSAY; BIOSYNTHESIS; FUNGAL STRAIN; GENE CLUSTER; GENE EXPRESSION; GENE SEQUENCE; MOLECULAR CLONING; NONHUMAN; STREPTOMYCES; STREPTOMYCES ANSOCHROMOGENES;

AMINOGLYCOSIDES; ANTIFUNGAL AGENTS; FUNGI; MAGNETIC RESONANCE SPECTROSCOPY; MOLECULAR CONFORMATION; MULTIGENE FAMILY; PLASMIDS; PYRIMIDINE NUCLEOSIDES; STREPTOMYCES; TANDEM MASS SPECTROMETRY;

FUNGI; HEXAPODA; STREPTOMYCES ANSOCHROMOGENES; STREPTOMYCES CACAOI;

EID: 84867036786     PISSN: None     EISSN: 14752859     Source Type: Journal    
DOI: 10.1186/1475-2859-11-135     Document Type: Article

References (24)

1. Isono K, Asahi K, Suzuki S. Studies on polyoxins, antifungal antibiotics. 13. The structure of polyoxins. J Am Chem Soc 1969, 91(26):7490-7505.
2. Hori M, Eguchi J, Kakiki K, Misato T. Studies on the mode of action of polyoxins. VI. Effect of polyoxin B on chitin synthesis in polyoxin-sensitive and resistant strains of Alternaria kikuchiana. J Antibiot 1974, 27(4):260-266.
3. Hori M, Kakiki K, Suzuki S, Misato T. Studies on the mode of action of polyoxins. Part III. Relation of polyoxin structure to chitin synthetase inhibition. Agr Biol Chem 1971, 35(8):1280-1291.
4. Endo A, Misato T. Polyoxin D, a competitive inhibitor of UDP-N-acetylglucosamine: chitin N-acetylglucosaminyltransferase in Neurospora crassa. Biochem Biophys Res Commun 1969, 37(4):718-722.
5. Isono K, Suzuki S. The structures of polyoxins A and B. Tetrahedron Lett 1968, 9:1133-1137.
6. Suzuki S, Isono K, Nagatsu J, Mizutani T, Kawashima Y, Mizuno T. Isolation and characterizaion of polyoxin J, K and L, new components of polyoxin complex. Agr Biol Chem 1968, 32(6):792-793.
7. Isono K, Nagatsu J, Kobinata K, Sasaki K, Suzuki S. Studies on polyoxins, antifungal antibiotics. Part V. Isolation and characterization of polyoxins C, D, E, F, G, H and I. Agr Biol Chem 1967, 31(2):190-199.
8. Suzuki S, Isono K, Nagatsu J, Mizutani T, Kawashima Y, Mizuno T. A new antibiotic, polyoxin A. J Antibiot 1965, 18:131.
9. Chen W, Huang T, He X, Meng Q, You D, Bai L, Li J, Wu M, Li R, Xie Z, Zhou H, Zhou X, Tan H, Deng Z. Characterization of the polyoxin biosynthetic gene cluster from Streptomyces cacaoi and engineered production of polyoxin H. J Biol Chem 2009, 284(16):10627-10638.
10. Chen W, Zeng H, Tan H. Cloning, sequencing, and function of sanF: a gene involved in nikkomycin biosynthesis of Streptomyces ansochromogenes. Curr Microbiol 2000, 41(5):312-316.
11. Delzer J, Fiedler HP, Muller H, Zahner H, Rathmann R, Ernst K, Konig WA. New nikkomycins by mutasynthesis and directed fermentation. J Antibiot 1984, 37(1):80-82.
12. Liao G, Li J, Li L, Yang H, Tian Y, Tan H. Cloning, reassembling and integration of the entire nikkomycin biosynthetic gene cluster into Streptomyces ansochromogenes lead to an improved nikkomycin production. Microb Cell Fact 2010, 9:6.
13. Li J, Li L, Tian Y, Niu G, Tan H. Hybrid antibiotics with the nikkomycin nucleoside and polyoxin peptidyl moieties. Metab Eng 2011, 13(3):336-344.
14. Ling H, Wang G, Li J, Tan H. sanN encoding a dehydrogenase is essential for nikkomycin biosynthesis in Streptomyces ansochromogenes. J Microbiol Biotechnol 2008, 18(3):397-403.
15. Ding W, Lei C, He Q, Zhang Q, Bi Y, Liu W. Insights into bacterial 6-methylsalicylic acid synthase and its engineering to orsellinic acid synthase for spirotetronate generation. Chem Biol 2010, 17(5):495-503.
16. Salas JA, Mendez C. Indolocarbazole antitumour compounds by combinatorial biosynthesis. Curr Opin Chem Biol 2009, 13(2):152-160.
17. Winter JM, Tang Y. Synthetic biological approaches to natural product biosynthesis. Curr Opin Biotechnol 2011, 23:1-8.
18. Kieser T, Bibb MJ, Buttner MJ, Chater KF, Hopwood DA. Practical Streptomyces Genetics 2000, Norwich: John Innes Foundation.
19. Sambrook J, Fritsch T, Maniatis EF. Molecular cloning: A laboratory Manual 2001, Cold Spring Harbor NY: Cold Spring Harbor Laboratory Press.
20. Gust B, Challis GL, Fowler K, Kieser T, Chater KF. PCR-targeted Streptomyces gene replacement identifies a protein domain needed for biosynthesis of the sesquiterpene soil odor geosmin. Proc Natl Acad Sci USA 2000, 100(4):1541-1546.
21. Li R, Xie Z, Tian Y, Yang H, Chen W, You D, Liu G, Deng Z, Tan H. polR, a pathway-specific transcriptional regulatory gene, positively controls polyoxin biosynthesis in Streptomyces cacaoi subsp. asoensis. Microbiology 2009, 155(6):1819-1831.
22. Li R, Liu G, Xie Z, He X, Chen W, Deng Z, Tan H. PolY, a transcriptional regulator with ATPase activity, directly activates transcription of polR in polyoxin biosynthesis in Streptomyces cacaoi. Mol Microbiol 2010, 75(2):349-364.
23. Schuz TC, Fiedler HP, Zahner H. Metabolic products of microorganisms. 263 Nikkomycin Sz, Sx, Soz and Sox, new intermediates associated to the nikkomycin biosynthesis of Streptomyces tendae. J Antibiot 1992, 45(2):199-206.
24. Liu G, Tian Y, Yang H, Tan H. A pathway-specific transcriptional regulatory gene for nikkomycin biosynthesis in Streptomyces ansochromogenes that also influences colony development. Mol Microbiol 2005, 55(6):1855-1866.