Endogenous Wnt signalling in human embryonic stem cells generates an equilibrium of distinct lineage-specified progenitors

Nature Communications

Volumn 3, Issue , 2012, Pages

  Blauwkamp, Timothy A ^a   Nigam, Shelly ^a   Ardehali, Reza ^b   Weissman, Irving L ^b   Nusse, Roel ^a  

^a STANFORD UNIVERSITY   (United States)

^b STANFORD UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

HEPATOCYTE NUCLEAR FACTOR 3BETA; LYSOPHOSPHATIDIC ACID; OCTAMER TRANSCRIPTION FACTOR 4; TRANSCRIPTION FACTOR GATA 4; WNT3A PROTEIN;

APOPTOSIS; ARTICLE; CELL DIFFERENTIATION; CELL EXPANSION; CELL LINEAGE; CELL PROLIFERATION; CELL REGENERATION; CELL RENEWAL; CELL SURVIVAL; CELL VIABILITY; CONTROLLED STUDY; EMBRYONIC STEM CELL; EPIGENETICS; GENE EXPRESSION; HEART MUSCLE CELL; HUMAN; HUMAN CELL; IN VITRO STUDY; MESODERM; NEUROECTODERM; PROTEIN EXPRESSION; SKIN CELL; WNT SIGNALING PATHWAY;

ANIMALS; CELL DIFFERENTIATION; CELL LINE; CELLS, CULTURED; EMBRYONIC STEM CELLS; FLOW CYTOMETRY; HUMANS; IMMUNOHISTOCHEMISTRY; MICE; SIGNAL TRANSDUCTION; WNT PROTEINS;

EID: 84867028809     PISSN: None     EISSN: 20411723     Source Type: Journal    
DOI: 10.1038/ncomms2064     Document Type: Article

References (34)

1. Stewart, M. H. et al. Clonal isolation of hESCs reveals heterogeneity within the pluripotent stem cell compartment. Nat. Methods 3, 807-815 (2006).
2. Hong, S. H. et al. Cell fate potential of human pluripotent stem cells is encoded by histone modifications. Cell Stem Cell 9, 24-36 (2011).
3. Hayashi, K., Lopes, S. M., Tang, F. & Surani, M. A. Dynamic equilibrium and heterogeneity of mouse pluripotent stem cells with distinct functional and epigenetic states. Cell Stem Cell 3, 391-401 (2008).
4. Han, D. W. et al. Epiblast stem cell subpopulations represent mouse embryos of distinct pregastrulation stages. Cell 143, 617-627 (2010).
5. D'Amour, K. A. et al. Production of pancreatic hormone-expressing endocrine cells from human embryonic stem cells. Nat. Biotechnol. 24, 1392-1401 (2006). (Pubitemid 44722103)
6. Lee, G. et al. Isolation and directed differentiation of neural crest stem cells derived from human embryonic stem cells. Nat. Biotechnol. 25, 1468-1475 (2007). (Pubitemid 350233140)
7. Cai, L. et al. Promoting human embryonic stem cell renewal or differentiation by modulating Wnt signal and culture conditions. Cell Res. 17, 62-72 (2007). (Pubitemid 46096426)
8. Dravid, G. et al. Defining the role of Wnt/beta-catenin signaling in the survival, proliferation, and self-renewal of human embryonic stem cells. Stem Cells (Dayton, Ohio) 23, 1489-1501 (2005). (Pubitemid 41740998)
9. Sato, N., Meijer, L., Skaltsounis, L., Greengard, P. & Brivanlou, A. H. Maintenance of pluripotency in human and mouse embryonic stem cells through activation of Wnt signaling by a pharmacological GSK-3-specific inhibitor. Nat. Med. 10, 55-63 (2004). (Pubitemid 38524699)
10. Sumi, T., Tsuneyoshi, N., Nakatsuji, N. & Suemori, H. Defining early lineage specification of human embryonic stem cells by the orchestrated balance of canonical Wnt/beta-catenin, Activin/Nodal and BMP signaling. Development (Cambridge, England) 135, 2969-2979 (2008).
11. Ullmann, U. et al. GSK-3-specific inhibitor-supplemented hESC medium prevents the epithelial-mesenchymal transition process and the up-regulation of matrix metalloproteinases in hESCs cultured in feeder-free conditions. Mol. Hum. Reprod. 14, 169-179 (2008). (Pubitemid 351404890)
12. Villa-Diaz, L. G. et al. Analysis of the factors that limit the ability of feeder-cells to maintain the undifferentiated state of human embryonic stem cells. Stem Cells Dev. 18, 641-651 (2009).
13. Davidson, K. C. et al. Wnt/β-catenin signaling promotes differentiation, not self-renewal, of human embryonic stem cells and is repressed by Oct4. Proc. Natl Acad. Sci. USA 109, 4485-4490 (2012).
14. ten Berge, D. et al. Embryonic stem cells require Wnt proteins to prevent differentiation to epiblast stem cells. Nat. Cell Biol. 13, 1070-1075 (2011).
15. Akopian, V. et al. Comparison of defined culture systems for feeder cell free propagation of human embryonic stem cells. In Vitro Cell Dev. Biol. Anim. 46, 247-258 (2010).
16. Yao, S. et al. Long-term self-renewal and directed differentiation of human embryonic stem cells in chemically defined conditions. Proc. Natl Acad. Sci. USA 103, 6907-6912 (2006).
17. Garcia-Gonzalo, F. R. & Izpisua Belmonte, J. C. Albumin-associated lipids regulate human embryonic stem cell self-renewal. PLoS One 3, e1384 (2008).
18. Berdichevets, I. N., Tyazhelova, T. V., Shimshilashvili Kh, R. & Rogaev, E. I. Lysophosphatidic acid is a lipid mediator with wide range of biological activities. Biosynthetic pathways and mechanism of action. Biochemistry (Mosc) 75, 1088-1097 (2010).
19. Kobayashi, T. et al. Effect of lysophosphatidic acid on the preimplantation development of mouse embryos. FEBS Lett. 351, 38-40 (1994). (Pubitemid 24268061)
20. Liu, Z. & Armant, D. R. Lysophosphatidic acid regulates murine blastocyst development by transactivation of receptors for heparin-binding EGF-like growth factor. Exp. Cell Res. 296, 317-326 (2004). (Pubitemid 38624652)
21. Ohta, H. et al. Ki16425, a subtype-selective antagonist for EDG-family lysophosphatidic acid receptors. Mol. Pharmacol. 64, 994-1005 (2003). (Pubitemid 37153068)
22. Arnold, S. J. & Robertson, E. J. Making a commitment: cell lineage allocation and axis patterning in the early mouse embryo. Nat. Rev. Mol. Cell Biol. 10, 91-103 (2009).
23. Currier, N. et al. Dynamic expression of a LEF-EGFP Wnt reporter in mouse development and cancer. Genesis 48, 183-194 (2010).
24. ten Berge, D. et al. Wnt signaling mediates self-organization and axis formation in embryoid bodies. Cell Stem Cell 3, 508-518 (2008).
25. Yamaguchi, T. P., Takada, S., Yoshikawa, Y., Wu, N. & McMahon, A. P. T (Brachyury) is a direct target of Wnt3a during paraxial mesoderm specification. Genes Dev. 13, 3185-3190 (1999). (Pubitemid 30030440)
26. Chambers, S. M. et al. Highly efficient neural conversion of human ES and iPS cells by dual inhibition of SMAD signaling. Nat. Biotechnol. 27, 275-280 (2009).
27. Yang, L. et al. Human cardiovascular progenitor cells develop from a KDR+ embryonic-stem-cell-derived population. Nature 453, 524-528 (2008). (Pubitemid 351733312)
28. Ardehali, R. et al. Prospective isolation of human embryonic stem cell-derived cardiovascular progenitors that integrate into the human fetal heart. Proc. Natl Acad. Sci. USA (in press) (2012).
29. Lyashenko, N. et al. Differential requirement for the dual functions of beta-catenin in embryonic stem cell self-renewal and germ layer formation. Nat. Cell Biol. 13, 753-761 (2011).
30. Yi, F. et al. Opposing effects of Tcf3 and Tcf1 control Wnt stimulation of embryonic stem cell self-renewal. Nat. Cell. Biol. 13, 762-770 (2011).
31. Wray, J. et al. Inhibition of glycogen synthase kinase-3 alleviates Tcf3 repression of the pluripotency network and increases embryonic stem cell resistance to differentiation. Nat. Cell Biol. 13, 838-845 (2011).
32. Willert, K. et al. Wnt proteins are lipid-modified and can act as stem cell growth factors. Nature 423, 448-452 (2003). (Pubitemid 36626997)
33. Watanabe, K. et al. A ROCK inhibitor permits survival of dissociated human embryonic stem cells. Nat. Biotechnol. 25, 681-686 (2007). (Pubitemid 46897207)
34. Hsieh J.-C., Rattner A., Smallwood P.M., Nathans J. Biochemical characterization of Wnt-frizzled interactions using a soluble, biologically active vertebrate Wnt protein. Proceedings of the National Academy of Sciences of the United States of America. 1999, 96(7), 3546-3551 DOI 10.1073/pnas.96.7. 3546.